Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species

Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species

This study aimed to establish the essential oil (EO) composition from young shoots of <i>Picea abies</i>, <i>Larix decidua</i>, <i>Pseudotsuga menziesii</i>, and <i>Pinus nigra</i> harvested from Romania and evaluate their antimicrobial and anti-virule...

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Autores principales: Diana-Carolina Visan, Eliza Oprea, Valeria Radulescu, Ion Voiculescu, Iovu-Adrian Biris, Ani Ioana Cotar, Crina Saviuc, Mariana Carmen Chifiriuc, Ioana Cristina Marinas
Formato: article
Lenguaje:EN
Publicado: MDPI AG 2021
Materias:
essential oil
antimicrobial activity
<i>Picea abies</i>
<i>Larix decidua</i>
<i>Pseudotsuga menziesii</i>
<i>Pinus nigra</i>
Medicine
R
Pharmacy and materia medica
RS1-441
Acceso en línea:https://doaj.org/article/7a27dc2133504c50829d45a014a7d1e2
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spelling oai:doaj.org-article:7a27dc2133504c50829d45a014a7d1e22021-11-25T18:39:49ZOriginal Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species10.3390/ph141111591424-8247https://doaj.org/article/7a27dc2133504c50829d45a014a7d1e22021-11-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1159https://doaj.org/toc/1424-8247This study aimed to establish the essential oil (EO) composition from young shoots of <i>Picea abies</i>, <i>Larix decidua</i>, <i>Pseudotsuga menziesii</i>, and <i>Pinus nigra</i> harvested from Romania and evaluate their antimicrobial and anti-virulence activity, as well as potential synergies with currently used antibiotics. The samples’ EO average content varied between 0.62% and 1.02% (mL/100 g plant). The mono- and sesquiterpene hydrocarbons were dominant in the composition of the studied EOs. The antimicrobial activity revealed that the minimum inhibitory concentration (MIC) values for the tested EOs and some pure compounds known for their antimicrobial activity ranged from 6.25 to 100 µL/mL. The most intensive antimicrobial effect was obtained for the <i>Pinus nigra</i> EO, which exhibited the best synergistic effect with some antibiotics against <i>Staphylococcus aureus</i> strains (i.e., oxacillin, tetracycline, erythromycin and gentamycin). The subinhibitory concentrations (sMIC) of the coniferous EOs inhibited the expression of soluble virulence factors (DN-ase, lipase, lecithinase, hemolysins, caseinase and siderophore-like), their efficiency being similar to that of the tested pure compounds, and inhibited the rhl gene expression in <i>Pseudomonas aeruginosa</i>, suggesting their virulence-arresting drug potential.Diana-Carolina VisanEliza OpreaValeria RadulescuIon VoiculescuIovu-Adrian BirisAni Ioana CotarCrina SaviucMariana Carmen ChifiriucIoana Cristina MarinasMDPI AGarticleessential oilantimicrobial activity<i>Picea abies</i><i>Larix decidua</i><i>Pseudotsuga menziesii</i><i>Pinus nigra</i>MedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1159, p 1159 (2021)
institution DOAJ
collection DOAJ
language EN
topic essential oil
antimicrobial activity
<i>Picea abies</i>
<i>Larix decidua</i>
<i>Pseudotsuga menziesii</i>
<i>Pinus nigra</i>
Medicine
R
Pharmacy and materia medica
RS1-441
spellingShingle essential oil
antimicrobial activity
<i>Picea abies</i>
<i>Larix decidua</i>
<i>Pseudotsuga menziesii</i>
<i>Pinus nigra</i>
Medicine
R
Pharmacy and materia medica
RS1-441
Diana-Carolina Visan
Eliza Oprea
Valeria Radulescu
Ion Voiculescu
Iovu-Adrian Biris
Ani Ioana Cotar
Crina Saviuc
Mariana Carmen Chifiriuc
Ioana Cristina Marinas
Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species
description This study aimed to establish the essential oil (EO) composition from young shoots of <i>Picea abies</i>, <i>Larix decidua</i>, <i>Pseudotsuga menziesii</i>, and <i>Pinus nigra</i> harvested from Romania and evaluate their antimicrobial and anti-virulence activity, as well as potential synergies with currently used antibiotics. The samples’ EO average content varied between 0.62% and 1.02% (mL/100 g plant). The mono- and sesquiterpene hydrocarbons were dominant in the composition of the studied EOs. The antimicrobial activity revealed that the minimum inhibitory concentration (MIC) values for the tested EOs and some pure compounds known for their antimicrobial activity ranged from 6.25 to 100 µL/mL. The most intensive antimicrobial effect was obtained for the <i>Pinus nigra</i> EO, which exhibited the best synergistic effect with some antibiotics against <i>Staphylococcus aureus</i> strains (i.e., oxacillin, tetracycline, erythromycin and gentamycin). The subinhibitory concentrations (sMIC) of the coniferous EOs inhibited the expression of soluble virulence factors (DN-ase, lipase, lecithinase, hemolysins, caseinase and siderophore-like), their efficiency being similar to that of the tested pure compounds, and inhibited the rhl gene expression in <i>Pseudomonas aeruginosa</i>, suggesting their virulence-arresting drug potential.
format article
author Diana-Carolina Visan
Eliza Oprea
Valeria Radulescu
Ion Voiculescu
Iovu-Adrian Biris
Ani Ioana Cotar
Crina Saviuc
Mariana Carmen Chifiriuc
Ioana Cristina Marinas
author_facet Diana-Carolina Visan
Eliza Oprea
Valeria Radulescu
Ion Voiculescu
Iovu-Adrian Biris
Ani Ioana Cotar
Crina Saviuc
Mariana Carmen Chifiriuc
Ioana Cristina Marinas
author_sort Diana-Carolina Visan
title Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species
title_short Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species
title_full Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species
title_fullStr Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species
title_full_unstemmed Original Contributions to the Chemical Composition, Microbicidal, Virulence-Arresting and Antibiotic-Enhancing Activity of Essential Oils from Four Coniferous Species
title_sort original contributions to the chemical composition, microbicidal, virulence-arresting and antibiotic-enhancing activity of essential oils from four coniferous species
publisher MDPI AG
publishDate 2021
url https://doaj.org/article/7a27dc2133504c50829d45a014a7d1e2
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