Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis

Abstract Dental plaque is a complex multispecies biofilm, and is a direct precursor of periodontal disease. The virulence of periodontal pathogens, such as Porphyromonas gingivalis, is expressed in the context of this polymicrobial community. Previously, we reported an antagonistic relationship betw...

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Autores principales:	Meng-Hsuan Ho, Richard J. Lamont, Hua Xie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a32f6d7689d46e7918bbd68124a4f51
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spelling	oai:doaj.org-article:7a32f6d7689d46e7918bbd68124a4f512021-12-02T11:52:41ZIdentification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis10.1038/s41598-017-01551-42045-2322https://doaj.org/article/7a32f6d7689d46e7918bbd68124a4f512017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01551-4https://doaj.org/toc/2045-2322Abstract Dental plaque is a complex multispecies biofilm, and is a direct precursor of periodontal disease. The virulence of periodontal pathogens, such as Porphyromonas gingivalis, is expressed in the context of this polymicrobial community. Previously, we reported an antagonistic relationship between Streptococcus cristatus and P. gingivalis, and identified arginine deiminase (ArcA) of S. cristatus as the signaling molecule to which P. gingivalis responds by repressing the expression and production of FimA protein. Here we demonstrate that direct interaction between P. gingivalis and S. cristatus is necessary for the cell-cell communication. Two surface proteins of P. gingivalis, PGN_0294 and PGN_0806, were found to interact with S. cristatus ArcA. Using a peptide array analysis, we identified several P. gingivalis-binding sites of ArcA, which led to the discovery of an 11-mer peptide with the native sequence of ArcA that repressed expression of fimbriae and of gingipains. These data indicate that a functional motif of ArcA is sufficient to selectively alter virulence gene expression in P. gingivalis, and PGN_0294 and PGN_0806 may serve as receptors for ArcA. Our findings provide a molecular basis for future rational design of agents that interfere with the initiation and formation of a P. gingivalis-induced pathogenic community.Meng-Hsuan HoRichard J. LamontHua XieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meng-Hsuan Ho Richard J. Lamont Hua Xie Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis
description	Abstract Dental plaque is a complex multispecies biofilm, and is a direct precursor of periodontal disease. The virulence of periodontal pathogens, such as Porphyromonas gingivalis, is expressed in the context of this polymicrobial community. Previously, we reported an antagonistic relationship between Streptococcus cristatus and P. gingivalis, and identified arginine deiminase (ArcA) of S. cristatus as the signaling molecule to which P. gingivalis responds by repressing the expression and production of FimA protein. Here we demonstrate that direct interaction between P. gingivalis and S. cristatus is necessary for the cell-cell communication. Two surface proteins of P. gingivalis, PGN_0294 and PGN_0806, were found to interact with S. cristatus ArcA. Using a peptide array analysis, we identified several P. gingivalis-binding sites of ArcA, which led to the discovery of an 11-mer peptide with the native sequence of ArcA that repressed expression of fimbriae and of gingipains. These data indicate that a functional motif of ArcA is sufficient to selectively alter virulence gene expression in P. gingivalis, and PGN_0294 and PGN_0806 may serve as receptors for ArcA. Our findings provide a molecular basis for future rational design of agents that interfere with the initiation and formation of a P. gingivalis-induced pathogenic community.
format	article
author	Meng-Hsuan Ho Richard J. Lamont Hua Xie
author_facet	Meng-Hsuan Ho Richard J. Lamont Hua Xie
author_sort	Meng-Hsuan Ho
title	Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis
title_short	Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis
title_full	Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis
title_fullStr	Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis
title_full_unstemmed	Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis
title_sort	identification of streptococcus cristatus peptides that repress expression of virulence genes in porphyromonas gingivalis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7a32f6d7689d46e7918bbd68124a4f51
work_keys_str_mv	AT menghsuanho identificationofstreptococcuscristatuspeptidesthatrepressexpressionofvirulencegenesinporphyromonasgingivalis AT richardjlamont identificationofstreptococcuscristatuspeptidesthatrepressexpressionofvirulencegenesinporphyromonasgingivalis AT huaxie identificationofstreptococcuscristatuspeptidesthatrepressexpressionofvirulencegenesinporphyromonasgingivalis
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Identification of Streptococcus cristatus peptides that repress expression of virulence genes in Porphyromonas gingivalis

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