Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast

Abstract Tumor immune microenvironment plays a crucial role in tumor progression. We performed immune profiling to compare immune-related gene expression between ductal carcinoma in situ (DCIS) and invasive carcinoma of the breast using nCounter PanCancer immune Profiling Panel and found that CXCL10...

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Autores principales:	Milim Kim, Hye Yeon Choi, Ji Won Woo, Yul Ri Chung, So Yeon Park
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a35859373714fccb865d1efbe04a89e
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spelling	oai:doaj.org-article:7a35859373714fccb865d1efbe04a89e2021-12-02T17:41:18ZRole of CXCL10 in the progression of in situ to invasive carcinoma of the breast10.1038/s41598-021-97390-52045-2322https://doaj.org/article/7a35859373714fccb865d1efbe04a89e2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97390-5https://doaj.org/toc/2045-2322Abstract Tumor immune microenvironment plays a crucial role in tumor progression. We performed immune profiling to compare immune-related gene expression between ductal carcinoma in situ (DCIS) and invasive carcinoma of the breast using nCounter PanCancer immune Profiling Panel and found that CXCL10 was the most significant gene that had the highest difference in expression between them. Effect of CXCL10 on breast cancer cell proliferation and invasion was examined in vitro, and expression of CXCL10 and its relationship with immune cell infiltration was assessed in breast cancer samples. CXCL10 induced cell proliferation, migration and epithelial-mesenchymal transition in MCF-7 and MDA-MB-231 breast cancer cell lines. We confirmed that CXCL10 mRNA expression was significantly higher in invasive carcinoma than in DCIS, especially in hormone receptor (HR)-negative tumors using a validation set. CXCL10 mRNA expression showed a positive correlation with tumor infiltrating lymphocyte (TIL) density in both DCIS and invasive carcinoma; CXCL10-positive tumors generally showed higher infiltration of CD8+ and FOXP3+TILs as well as PD-L1+ immune cells compared to CXCL10-negative tumors, albeit with different patterns according to HR status. In conclusion, our study showed that CXCL10 promotes tumor cell proliferation, invasion, and immune cell infiltration, implying its contribution in the progression of DCIS to invasive carcinoma of the breast.Milim KimHye Yeon ChoiJi Won WooYul Ri ChungSo Yeon ParkNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Milim Kim Hye Yeon Choi Ji Won Woo Yul Ri Chung So Yeon Park Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast
description	Abstract Tumor immune microenvironment plays a crucial role in tumor progression. We performed immune profiling to compare immune-related gene expression between ductal carcinoma in situ (DCIS) and invasive carcinoma of the breast using nCounter PanCancer immune Profiling Panel and found that CXCL10 was the most significant gene that had the highest difference in expression between them. Effect of CXCL10 on breast cancer cell proliferation and invasion was examined in vitro, and expression of CXCL10 and its relationship with immune cell infiltration was assessed in breast cancer samples. CXCL10 induced cell proliferation, migration and epithelial-mesenchymal transition in MCF-7 and MDA-MB-231 breast cancer cell lines. We confirmed that CXCL10 mRNA expression was significantly higher in invasive carcinoma than in DCIS, especially in hormone receptor (HR)-negative tumors using a validation set. CXCL10 mRNA expression showed a positive correlation with tumor infiltrating lymphocyte (TIL) density in both DCIS and invasive carcinoma; CXCL10-positive tumors generally showed higher infiltration of CD8+ and FOXP3+TILs as well as PD-L1+ immune cells compared to CXCL10-negative tumors, albeit with different patterns according to HR status. In conclusion, our study showed that CXCL10 promotes tumor cell proliferation, invasion, and immune cell infiltration, implying its contribution in the progression of DCIS to invasive carcinoma of the breast.
format	article
author	Milim Kim Hye Yeon Choi Ji Won Woo Yul Ri Chung So Yeon Park
author_facet	Milim Kim Hye Yeon Choi Ji Won Woo Yul Ri Chung So Yeon Park
author_sort	Milim Kim
title	Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast
title_short	Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast
title_full	Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast
title_fullStr	Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast
title_full_unstemmed	Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast
title_sort	role of cxcl10 in the progression of in situ to invasive carcinoma of the breast
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7a35859373714fccb865d1efbe04a89e
work_keys_str_mv	AT milimkim roleofcxcl10intheprogressionofinsitutoinvasivecarcinomaofthebreast AT hyeyeonchoi roleofcxcl10intheprogressionofinsitutoinvasivecarcinomaofthebreast AT jiwonwoo roleofcxcl10intheprogressionofinsitutoinvasivecarcinomaofthebreast AT yulrichung roleofcxcl10intheprogressionofinsitutoinvasivecarcinomaofthebreast AT soyeonpark roleofcxcl10intheprogressionofinsitutoinvasivecarcinomaofthebreast
_version_	1718379713254129664

Role of CXCL10 in the progression of in situ to invasive carcinoma of the breast

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