Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.

In this work highly localized femtosecond laser ablation is used to dissect single axons within a living Caenorhabditis elegans (C. elegans). We present a multimodal imaging methodology for the assessment of the collateral damage induced by the laser. This relies on the observation of the tissues su...

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Autores principales:	Susana I C O Santos, Manoj Mathew, Omar E Olarte, Sotiris Psilodimitrakopoulos, Pablo Loza-Alvarez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a373b4d38574e6fa848d2721d765dc5
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spelling	oai:doaj.org-article:7a373b4d38574e6fa848d2721d765dc52021-11-18T07:54:31ZFemtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.1932-620310.1371/journal.pone.0058600https://doaj.org/article/7a373b4d38574e6fa848d2721d765dc52013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23484040/?tool=EBIhttps://doaj.org/toc/1932-6203In this work highly localized femtosecond laser ablation is used to dissect single axons within a living Caenorhabditis elegans (C. elegans). We present a multimodal imaging methodology for the assessment of the collateral damage induced by the laser. This relies on the observation of the tissues surrounding the targeted region using a combination of different high resolution microscopy modalities. We present the use of Second Harmonic Generation (SHG) and Polarization Sensitive SHG (PSHG) to determine damage in the neighbor muscle cells. All the above is done using a single instrument: multimodal microscopy setup that allows simultaneous imaging in the linear and non-linear regimes and femtosecond-laser ablation.Susana I C O SantosManoj MathewOmar E OlarteSotiris PsilodimitrakopoulosPablo Loza-AlvarezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e58600 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Susana I C O Santos Manoj Mathew Omar E Olarte Sotiris Psilodimitrakopoulos Pablo Loza-Alvarez Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.
description	In this work highly localized femtosecond laser ablation is used to dissect single axons within a living Caenorhabditis elegans (C. elegans). We present a multimodal imaging methodology for the assessment of the collateral damage induced by the laser. This relies on the observation of the tissues surrounding the targeted region using a combination of different high resolution microscopy modalities. We present the use of Second Harmonic Generation (SHG) and Polarization Sensitive SHG (PSHG) to determine damage in the neighbor muscle cells. All the above is done using a single instrument: multimodal microscopy setup that allows simultaneous imaging in the linear and non-linear regimes and femtosecond-laser ablation.
format	article
author	Susana I C O Santos Manoj Mathew Omar E Olarte Sotiris Psilodimitrakopoulos Pablo Loza-Alvarez
author_facet	Susana I C O Santos Manoj Mathew Omar E Olarte Sotiris Psilodimitrakopoulos Pablo Loza-Alvarez
author_sort	Susana I C O Santos
title	Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.
title_short	Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.
title_full	Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.
title_fullStr	Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.
title_full_unstemmed	Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.
title_sort	femtosecond laser axotomy in caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7a373b4d38574e6fa848d2721d765dc5
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Femtosecond laser axotomy in Caenorhabditis elegans and collateral damage assessment using a combination of linear and nonlinear imaging techniques.

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