Drug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin

BACKGROUND AND OBJECTIVE: Tuberculosis is the most dangerous infectious disease in the world today. Rifampicin (RMP) and Isoniazid (INH) are the two most important first-line anti-tuberculosis drugs for the treatment of tuberculosis. Resistance to these drugs has been increased in most of countries....

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Autores principales:	M Pourhajibagher, M Nasrollahi, SR Musavi, B Rahimi-Esboei, A Ghorbani Pashakolaei
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2012
Materias:	rifampin isoniazid mycobacterium tuberculosis drug resistance Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7a3acb9c5a264b3f83edfd7ddaab2e28
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spelling	oai:doaj.org-article:7a3acb9c5a264b3f83edfd7ddaab2e282021-11-10T08:53:11ZDrug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin1561-41072251-7170https://doaj.org/article/7a3acb9c5a264b3f83edfd7ddaab2e282012-05-01T00:00:00Zhttp://jbums.org/article-1-4092-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Tuberculosis is the most dangerous infectious disease in the world today. Rifampicin (RMP) and Isoniazid (INH) are the two most important first-line anti-tuberculosis drugs for the treatment of tuberculosis. Resistance to these drugs has been increased in most of countries. The aim of this study was evaluation of drug resistant in mycobacterium tuberculosis isolates to Isoniazid and Rifampin in patients that referred to health care center of Mazandaran province, Iran.METHODS: In this cross-sectional study, 1345 samples were collected of patients with clinical suspicions of tuberculosis that referred to health care centre of Mazandaran from July 2010 to June 2011. The specimens were cultured on Lowenstein-Jensen medium to detect the mycobacteria. DNA extraction of colonies and resistance to Isoniazid and Rifampin were evaluated by using the primers of inhA, KatG and rpoB genes.FINDINGS: Of 1345 specimens, only 65 isolates were positive culture. Out of 65, 59 were MTBC by using the primers of MUTB-gyrB gene. Among this isolates, 56 (94.92%), 55 (93.23%) and 58 (98.3%) were susceptible and 3 (5.08%), 4 (6.77%) and 1 (1.7%) were resistant to inhA, KatG and rpoB, respectively. CONCLUSION: The most resistance has been associated to Isoniazid. PCR can be a good method for identifying of the resistant strains of mycobacterium.M PourhajibagherM NasrollahiSR MusaviB Rahimi-EsboeiA Ghorbani PashakolaeiBabol University of Medical Sciencesarticlerifampinisoniazidmycobacterium tuberculosisdrug resistanceMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 14, Iss 3, Pp 66-72 (2012)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	rifampin isoniazid mycobacterium tuberculosis drug resistance Medicine R Medicine (General) R5-920
spellingShingle	rifampin isoniazid mycobacterium tuberculosis drug resistance Medicine R Medicine (General) R5-920 M Pourhajibagher M Nasrollahi SR Musavi B Rahimi-Esboei A Ghorbani Pashakolaei Drug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin
description	BACKGROUND AND OBJECTIVE: Tuberculosis is the most dangerous infectious disease in the world today. Rifampicin (RMP) and Isoniazid (INH) are the two most important first-line anti-tuberculosis drugs for the treatment of tuberculosis. Resistance to these drugs has been increased in most of countries. The aim of this study was evaluation of drug resistant in mycobacterium tuberculosis isolates to Isoniazid and Rifampin in patients that referred to health care center of Mazandaran province, Iran.METHODS: In this cross-sectional study, 1345 samples were collected of patients with clinical suspicions of tuberculosis that referred to health care centre of Mazandaran from July 2010 to June 2011. The specimens were cultured on Lowenstein-Jensen medium to detect the mycobacteria. DNA extraction of colonies and resistance to Isoniazid and Rifampin were evaluated by using the primers of inhA, KatG and rpoB genes.FINDINGS: Of 1345 specimens, only 65 isolates were positive culture. Out of 65, 59 were MTBC by using the primers of MUTB-gyrB gene. Among this isolates, 56 (94.92%), 55 (93.23%) and 58 (98.3%) were susceptible and 3 (5.08%), 4 (6.77%) and 1 (1.7%) were resistant to inhA, KatG and rpoB, respectively. CONCLUSION: The most resistance has been associated to Isoniazid. PCR can be a good method for identifying of the resistant strains of mycobacterium.
format	article
author	M Pourhajibagher M Nasrollahi SR Musavi B Rahimi-Esboei A Ghorbani Pashakolaei
author_facet	M Pourhajibagher M Nasrollahi SR Musavi B Rahimi-Esboei A Ghorbani Pashakolaei
author_sort	M Pourhajibagher
title	Drug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin
title_short	Drug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin
title_full	Drug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin
title_fullStr	Drug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin
title_full_unstemmed	Drug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin
title_sort	drug resistance in mycobacterium tuberculosis isolates to isoniazid and rifampin
publisher	Babol University of Medical Sciences
publishDate	2012
url	https://doaj.org/article/7a3acb9c5a264b3f83edfd7ddaab2e28
work_keys_str_mv	AT mpourhajibagher drugresistanceinmycobacteriumtuberculosisisolatestoisoniazidandrifampin AT mnasrollahi drugresistanceinmycobacteriumtuberculosisisolatestoisoniazidandrifampin AT srmusavi drugresistanceinmycobacteriumtuberculosisisolatestoisoniazidandrifampin AT brahimiesboei drugresistanceinmycobacteriumtuberculosisisolatestoisoniazidandrifampin AT aghorbanipashakolaei drugresistanceinmycobacteriumtuberculosisisolatestoisoniazidandrifampin
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Drug Resistance in Mycobacterium Tuberculosis Isolates to Isoniazid and Rifampin

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