Bacillus subtilis ANSB168 Producing d-alanyl-d-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A

Ochratoxin A (OTA) is toxic to animals and threatens food safety through residues in animal tissues. A novel degrading strain Bacillus subtilis ANSB168 was isolated and further investigated. We cloned d-alanyl-&l...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hanrui Qing, Xueting Huo, Shimeng Huang, Lihong Zhao, Jianyun Zhang, Cheng Ji, Qiugang Ma
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	ochratoxin A mycotoxin degradation carboxypeptidase poultry Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/7a3c0386d42541a590ff2be930b4f165
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7a3c0386d42541a590ff2be930b4f165
record_format	dspace
spelling	oai:doaj.org-article:7a3c0386d42541a590ff2be930b4f1652021-11-11T17:27:21Z<i>Bacillus subtilis</i> ANSB168 Producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A10.3390/ijms2221120591422-00671661-6596https://doaj.org/article/7a3c0386d42541a590ff2be930b4f1652021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/12059https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Ochratoxin A (OTA) is toxic to animals and threatens food safety through residues in animal tissues. A novel degrading strain <i>Bacillus subtilis</i> ANSB168 was isolated and further investigated. We cloned <span style="font-variant: small-caps;">d</span>-alanyl-<span style="font-variant: small-caps;">d</span>-alanine carboxypeptidase DacA and DacB from ANSB168 and over-expressed them in <i>Escherichia coli Rosetta</i> (DE3). Then, we characterized the OTA degradation mechanism of DacA and DacB, which was degrading OTA into OTα. A total of 45 laying hens were divided into three equal groups. The control group was fed basal feed, and other groups were administered with OTA (250 μg/kg of feed). A freeze-dried culture powder of ANSB168 (3 × 10<sup>7</sup> CFU/g, 2 kg/T of feed) was added to one of the OTA-fed groups for 28 days from day one of the experiment. We found that OTA significantly damaged the kidney and liver, inducing inflammation and activating the humoral immune system, causing oxidative stress in the layers. The ANSB168 bioproduct was able to alleviate OTA-induced kidney and liver damage, relieving OTA-induced inflammation and oxidative stress. Overall, DacA and DacB derived from ANSB168 degraded OTA into OTα, while the ANSB168 bioproduct was able to alleviate damages induced by OTA in laying hens.Hanrui QingXueting HuoShimeng HuangLihong ZhaoJianyun ZhangCheng JiQiugang MaMDPI AGarticleochratoxin AmycotoxindegradationcarboxypeptidasepoultryBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12059, p 12059 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ochratoxin A mycotoxin degradation carboxypeptidase poultry Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	ochratoxin A mycotoxin degradation carboxypeptidase poultry Biology (General) QH301-705.5 Chemistry QD1-999 Hanrui Qing Xueting Huo Shimeng Huang Lihong Zhao Jianyun Zhang Cheng Ji Qiugang Ma <i>Bacillus subtilis</i> ANSB168 Producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A
description	Ochratoxin A (OTA) is toxic to animals and threatens food safety through residues in animal tissues. A novel degrading strain <i>Bacillus subtilis</i> ANSB168 was isolated and further investigated. We cloned <span style="font-variant: small-caps;">d</span>-alanyl-<span style="font-variant: small-caps;">d</span>-alanine carboxypeptidase DacA and DacB from ANSB168 and over-expressed them in <i>Escherichia coli Rosetta</i> (DE3). Then, we characterized the OTA degradation mechanism of DacA and DacB, which was degrading OTA into OTα. A total of 45 laying hens were divided into three equal groups. The control group was fed basal feed, and other groups were administered with OTA (250 μg/kg of feed). A freeze-dried culture powder of ANSB168 (3 × 10<sup>7</sup> CFU/g, 2 kg/T of feed) was added to one of the OTA-fed groups for 28 days from day one of the experiment. We found that OTA significantly damaged the kidney and liver, inducing inflammation and activating the humoral immune system, causing oxidative stress in the layers. The ANSB168 bioproduct was able to alleviate OTA-induced kidney and liver damage, relieving OTA-induced inflammation and oxidative stress. Overall, DacA and DacB derived from ANSB168 degraded OTA into OTα, while the ANSB168 bioproduct was able to alleviate damages induced by OTA in laying hens.
format	article
author	Hanrui Qing Xueting Huo Shimeng Huang Lihong Zhao Jianyun Zhang Cheng Ji Qiugang Ma
author_facet	Hanrui Qing Xueting Huo Shimeng Huang Lihong Zhao Jianyun Zhang Cheng Ji Qiugang Ma
author_sort	Hanrui Qing
title	<i>Bacillus subtilis</i> ANSB168 Producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A
title_short	<i>Bacillus subtilis</i> ANSB168 Producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A
title_full	<i>Bacillus subtilis</i> ANSB168 Producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A
title_fullStr	<i>Bacillus subtilis</i> ANSB168 Producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A
title_full_unstemmed	<i>Bacillus subtilis</i> ANSB168 Producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A
title_sort	<i>bacillus subtilis</i> ansb168 producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine carboxypeptidase could alleviate the immune injury and inflammation induced by ochratoxin a
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7a3c0386d42541a590ff2be930b4f165
work_keys_str_mv	AT hanruiqing ibacillussubtilisiansb168producingspanstylefontvariantsmallcapsdspanalanylspanstylefontvariantsmallcapsdspanalaninecarboxypeptidasecouldalleviatetheimmuneinjuryandinflammationinducedbyochratoxina AT xuetinghuo ibacillussubtilisiansb168producingspanstylefontvariantsmallcapsdspanalanylspanstylefontvariantsmallcapsdspanalaninecarboxypeptidasecouldalleviatetheimmuneinjuryandinflammationinducedbyochratoxina AT shimenghuang ibacillussubtilisiansb168producingspanstylefontvariantsmallcapsdspanalanylspanstylefontvariantsmallcapsdspanalaninecarboxypeptidasecouldalleviatetheimmuneinjuryandinflammationinducedbyochratoxina AT lihongzhao ibacillussubtilisiansb168producingspanstylefontvariantsmallcapsdspanalanylspanstylefontvariantsmallcapsdspanalaninecarboxypeptidasecouldalleviatetheimmuneinjuryandinflammationinducedbyochratoxina AT jianyunzhang ibacillussubtilisiansb168producingspanstylefontvariantsmallcapsdspanalanylspanstylefontvariantsmallcapsdspanalaninecarboxypeptidasecouldalleviatetheimmuneinjuryandinflammationinducedbyochratoxina AT chengji ibacillussubtilisiansb168producingspanstylefontvariantsmallcapsdspanalanylspanstylefontvariantsmallcapsdspanalaninecarboxypeptidasecouldalleviatetheimmuneinjuryandinflammationinducedbyochratoxina AT qiugangma ibacillussubtilisiansb168producingspanstylefontvariantsmallcapsdspanalanylspanstylefontvariantsmallcapsdspanalaninecarboxypeptidasecouldalleviatetheimmuneinjuryandinflammationinducedbyochratoxina
_version_	1718432070920830976

<i>Bacillus subtilis</i> ANSB168 Producing <span style="font-variant: small-caps">d</span>-alanyl-<span style="font-variant: small-caps">d</span>-alanine Carboxypeptidase Could Alleviate the Immune Injury and Inflammation Induced by Ochratoxin A

Ejemplares similares