Characterization of Prdm9 in equids and sterility in mules.

Prdm9 (Meisetz) is the first speciation gene discovered in vertebrates conferring reproductive isolation. This locus encodes a meiosis-specific histone H3 methyltransferase that specifies meiotic recombination hotspots during gametogenesis. Allelic differences in Prdm9, characterized for a variable...

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Autores principales:	Cynthia C Steiner, Oliver A Ryder
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a3dc81b34f24822bf00687ae5583b57
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spelling	oai:doaj.org-article:7a3dc81b34f24822bf00687ae5583b572021-11-18T07:48:37ZCharacterization of Prdm9 in equids and sterility in mules.1932-620310.1371/journal.pone.0061746https://doaj.org/article/7a3dc81b34f24822bf00687ae5583b572013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23613924/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Prdm9 (Meisetz) is the first speciation gene discovered in vertebrates conferring reproductive isolation. This locus encodes a meiosis-specific histone H3 methyltransferase that specifies meiotic recombination hotspots during gametogenesis. Allelic differences in Prdm9, characterized for a variable number of zinc finger (ZF) domains, have been associated with hybrid sterility in male house mice via spermatogenic failure at the pachytene stage. The mule, a classic example of hybrid sterility in mammals also exhibits a similar spermatogenesis breakdown, making Prdm9 an interesting candidate to evaluate in equine hybrids. In this study, we characterized the Prdm9 gene in all species of equids by analyzing sequence variation of the ZF domains and estimating positive selection. We also evaluated the role of Prdm9 in hybrid sterility by assessing allelic differences of ZF domains in equine hybrids. We found remarkable variation in the sequence and number of ZF domains among equid species, ranging from five domains in the Tibetan kiang and Asiatic wild ass, to 14 in the Grevy's zebra. Positive selection was detected in all species at amino acid sites known to be associated with DNA-binding specificity of ZF domains in mice and humans. Equine hybrids, in particular a quartet pedigree composed of a fertile mule showed a mosaic of sequences and number of ZF domains suggesting that Prdm9 variation does not seem by itself to contribute to equine hybrid sterility.Cynthia C SteinerOliver A RyderPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 4, p e61746 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cynthia C Steiner Oliver A Ryder Characterization of Prdm9 in equids and sterility in mules.
description	Prdm9 (Meisetz) is the first speciation gene discovered in vertebrates conferring reproductive isolation. This locus encodes a meiosis-specific histone H3 methyltransferase that specifies meiotic recombination hotspots during gametogenesis. Allelic differences in Prdm9, characterized for a variable number of zinc finger (ZF) domains, have been associated with hybrid sterility in male house mice via spermatogenic failure at the pachytene stage. The mule, a classic example of hybrid sterility in mammals also exhibits a similar spermatogenesis breakdown, making Prdm9 an interesting candidate to evaluate in equine hybrids. In this study, we characterized the Prdm9 gene in all species of equids by analyzing sequence variation of the ZF domains and estimating positive selection. We also evaluated the role of Prdm9 in hybrid sterility by assessing allelic differences of ZF domains in equine hybrids. We found remarkable variation in the sequence and number of ZF domains among equid species, ranging from five domains in the Tibetan kiang and Asiatic wild ass, to 14 in the Grevy's zebra. Positive selection was detected in all species at amino acid sites known to be associated with DNA-binding specificity of ZF domains in mice and humans. Equine hybrids, in particular a quartet pedigree composed of a fertile mule showed a mosaic of sequences and number of ZF domains suggesting that Prdm9 variation does not seem by itself to contribute to equine hybrid sterility.
format	article
author	Cynthia C Steiner Oliver A Ryder
author_facet	Cynthia C Steiner Oliver A Ryder
author_sort	Cynthia C Steiner
title	Characterization of Prdm9 in equids and sterility in mules.
title_short	Characterization of Prdm9 in equids and sterility in mules.
title_full	Characterization of Prdm9 in equids and sterility in mules.
title_fullStr	Characterization of Prdm9 in equids and sterility in mules.
title_full_unstemmed	Characterization of Prdm9 in equids and sterility in mules.
title_sort	characterization of prdm9 in equids and sterility in mules.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7a3dc81b34f24822bf00687ae5583b57
work_keys_str_mv	AT cynthiacsteiner characterizationofprdm9inequidsandsterilityinmules AT oliveraryder characterizationofprdm9inequidsandsterilityinmules
_version_	1718422919642611712

Characterization of Prdm9 in equids and sterility in mules.

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