First detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana.

<h4>Background</h4>Leishmania major and an uncharacterized species have been reported from human patients in a cutaneous leishmaniasis (CL) outbreak area in Ghana. Reports from the area indicate the presence of anthropophilic Sergentomyia species that were found with Leishmania DNA.<h...

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Autores principales: Chukwunonso O Nzelu, Hirotomo Kato, Naiki Puplampu, Kwame Desewu, Shirley Odoom, Michael D Wilson, Tatsuya Sakurai, Ken Katakura, Daniel A Boakye
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Publicado: Public Library of Science (PLoS) 2014
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spelling oai:doaj.org-article:7a40c7cba89744d9bef8998e8b5da2392021-11-18T09:16:26ZFirst detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana.1935-27271935-273510.1371/journal.pntd.0002630https://doaj.org/article/7a40c7cba89744d9bef8998e8b5da2392014-02-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24516676/?tool=EBIhttps://doaj.org/toc/1935-2727https://doaj.org/toc/1935-2735<h4>Background</h4>Leishmania major and an uncharacterized species have been reported from human patients in a cutaneous leishmaniasis (CL) outbreak area in Ghana. Reports from the area indicate the presence of anthropophilic Sergentomyia species that were found with Leishmania DNA.<h4>Methodology/principal findings</h4>In this study, we analyzed the Leishmania DNA positive sand fly pools by PCR-RFLP and ITS1 gene sequencing. The trypanosome was determined using the SSU rRNA gene sequence. We observed DNA of L. major, L. tropica and Trypanosoma species to be associated with the sand fly infections. This study provides the first detection of L. tropica DNA and Trypanosoma species as well as the confirmation of L. major DNA within Sergentomyia sand flies in Ghana and suggests that S. ingrami and S. hamoni are possible vectors of CL in the study area.<h4>Conclusions/significance</h4>The detection of L. tropica DNA in this CL focus is a novel finding in Ghana as well as West Africa. In addition, the unexpected infection of Trypanosoma DNA within S. africana africana indicates that more attention is necessary when identifying parasitic organisms by PCR within sand fly vectors in Ghana and other areas where leishmaniasis is endemic.Chukwunonso O NzeluHirotomo KatoNaiki PuplampuKwame DesewuShirley OdoomMichael D WilsonTatsuya SakuraiKen KatakuraDaniel A BoakyePublic Library of Science (PLoS)articleArctic medicine. Tropical medicineRC955-962Public aspects of medicineRA1-1270ENPLoS Neglected Tropical Diseases, Vol 8, Iss 2, p e2630 (2014)
institution DOAJ
collection DOAJ
language EN
topic Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Chukwunonso O Nzelu
Hirotomo Kato
Naiki Puplampu
Kwame Desewu
Shirley Odoom
Michael D Wilson
Tatsuya Sakurai
Ken Katakura
Daniel A Boakye
First detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana.
description <h4>Background</h4>Leishmania major and an uncharacterized species have been reported from human patients in a cutaneous leishmaniasis (CL) outbreak area in Ghana. Reports from the area indicate the presence of anthropophilic Sergentomyia species that were found with Leishmania DNA.<h4>Methodology/principal findings</h4>In this study, we analyzed the Leishmania DNA positive sand fly pools by PCR-RFLP and ITS1 gene sequencing. The trypanosome was determined using the SSU rRNA gene sequence. We observed DNA of L. major, L. tropica and Trypanosoma species to be associated with the sand fly infections. This study provides the first detection of L. tropica DNA and Trypanosoma species as well as the confirmation of L. major DNA within Sergentomyia sand flies in Ghana and suggests that S. ingrami and S. hamoni are possible vectors of CL in the study area.<h4>Conclusions/significance</h4>The detection of L. tropica DNA in this CL focus is a novel finding in Ghana as well as West Africa. In addition, the unexpected infection of Trypanosoma DNA within S. africana africana indicates that more attention is necessary when identifying parasitic organisms by PCR within sand fly vectors in Ghana and other areas where leishmaniasis is endemic.
format article
author Chukwunonso O Nzelu
Hirotomo Kato
Naiki Puplampu
Kwame Desewu
Shirley Odoom
Michael D Wilson
Tatsuya Sakurai
Ken Katakura
Daniel A Boakye
author_facet Chukwunonso O Nzelu
Hirotomo Kato
Naiki Puplampu
Kwame Desewu
Shirley Odoom
Michael D Wilson
Tatsuya Sakurai
Ken Katakura
Daniel A Boakye
author_sort Chukwunonso O Nzelu
title First detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana.
title_short First detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana.
title_full First detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana.
title_fullStr First detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana.
title_full_unstemmed First detection of Leishmania tropica DNA and Trypanosoma species in Sergentomyia sand flies (Diptera: Psychodidae) from an outbreak area of cutaneous leishmaniasis in Ghana.
title_sort first detection of leishmania tropica dna and trypanosoma species in sergentomyia sand flies (diptera: psychodidae) from an outbreak area of cutaneous leishmaniasis in ghana.
publisher Public Library of Science (PLoS)
publishDate 2014
url https://doaj.org/article/7a40c7cba89744d9bef8998e8b5da239
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