Benchtop micro-mashing: high-throughput, robust, experimental beer brewing

Abstract Brewing science is undergoing a renaissance with the use of modern analytical chemistry and microbiology techniques. However, these modern analytical tools and techniques are not necessarily aligned with the scale and scope of brewing science. In particular, brewing processes can be time co...

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Autores principales:	Edward D. Kerr, Christopher H. Caboche, Peter Josh, Benjamin L. Schulz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a44003eb3d74f3bb6ddf302bd25d2a9
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id	oai:doaj.org-article:7a44003eb3d74f3bb6ddf302bd25d2a9
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spelling	oai:doaj.org-article:7a44003eb3d74f3bb6ddf302bd25d2a92021-12-02T15:23:11ZBenchtop micro-mashing: high-throughput, robust, experimental beer brewing10.1038/s41598-020-80442-72045-2322https://doaj.org/article/7a44003eb3d74f3bb6ddf302bd25d2a92021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80442-7https://doaj.org/toc/2045-2322Abstract Brewing science is undergoing a renaissance with the use of modern analytical chemistry and microbiology techniques. However, these modern analytical tools and techniques are not necessarily aligned with the scale and scope of brewing science. In particular, brewing processes can be time consuming, ingredient intensive, and require specialised technical equipment. These drawbacks compound with the need for appropriate numbers of replicates for adequately powered experimental design. Here, we describe a micro-scale mash method that can be performed using a common laboratory benchtop shaker/incubator, allowing for high throughput mashing and easy sample replication for statistical analysis. Proteomic profiles at both the protein and peptide levels were consistent between the 1 mL micro-mash and a 23 L Braumeister mash, and both mash scales produced wort with equivalent fermentable sugar and free amino acid profiles. The experimental flexibility offered by our micro-mash method allowed us to investigate the effects of altered mash parameters on the beer brewing proteome.Edward D. KerrChristopher H. CabochePeter JoshBenjamin L. SchulzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Edward D. Kerr Christopher H. Caboche Peter Josh Benjamin L. Schulz Benchtop micro-mashing: high-throughput, robust, experimental beer brewing
description	Abstract Brewing science is undergoing a renaissance with the use of modern analytical chemistry and microbiology techniques. However, these modern analytical tools and techniques are not necessarily aligned with the scale and scope of brewing science. In particular, brewing processes can be time consuming, ingredient intensive, and require specialised technical equipment. These drawbacks compound with the need for appropriate numbers of replicates for adequately powered experimental design. Here, we describe a micro-scale mash method that can be performed using a common laboratory benchtop shaker/incubator, allowing for high throughput mashing and easy sample replication for statistical analysis. Proteomic profiles at both the protein and peptide levels were consistent between the 1 mL micro-mash and a 23 L Braumeister mash, and both mash scales produced wort with equivalent fermentable sugar and free amino acid profiles. The experimental flexibility offered by our micro-mash method allowed us to investigate the effects of altered mash parameters on the beer brewing proteome.
format	article
author	Edward D. Kerr Christopher H. Caboche Peter Josh Benjamin L. Schulz
author_facet	Edward D. Kerr Christopher H. Caboche Peter Josh Benjamin L. Schulz
author_sort	Edward D. Kerr
title	Benchtop micro-mashing: high-throughput, robust, experimental beer brewing
title_short	Benchtop micro-mashing: high-throughput, robust, experimental beer brewing
title_full	Benchtop micro-mashing: high-throughput, robust, experimental beer brewing
title_fullStr	Benchtop micro-mashing: high-throughput, robust, experimental beer brewing
title_full_unstemmed	Benchtop micro-mashing: high-throughput, robust, experimental beer brewing
title_sort	benchtop micro-mashing: high-throughput, robust, experimental beer brewing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7a44003eb3d74f3bb6ddf302bd25d2a9
work_keys_str_mv	AT edwarddkerr benchtopmicromashinghighthroughputrobustexperimentalbeerbrewing AT christopherhcaboche benchtopmicromashinghighthroughputrobustexperimentalbeerbrewing AT peterjosh benchtopmicromashinghighthroughputrobustexperimentalbeerbrewing AT benjaminlschulz benchtopmicromashinghighthroughputrobustexperimentalbeerbrewing
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Benchtop micro-mashing: high-throughput, robust, experimental beer brewing

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