Multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy

To date, the use of nanocarriers has been developed in various fields, especially in cancer treatment. Graphene oxide (GO) is a novel drug delivery system that eagerly attracts the attention of many researchers due to its unique features. For the first time, a biocompatible AS1411 APT-GO-COOH was sy...

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Autores principales:	Yaghoubi Fatemeh, Naghib Seyed Morteza, Motlagh Najmeh Sadat Hosseini, Haghiralsadat Fateme, Jaliani Hossein Zarei, Tofighi Davood, Moradi Ali
Formato:	article
Lenguaje:	EN
Publicado:	De Gruyter 2021
Materias:	graphene oxide curcumin doxorubicin aptamer cell culture drug delivery nanoparticle Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801
Acceso en línea:	https://doaj.org/article/7a46c9901e404d679b9184b028c319df
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spelling	oai:doaj.org-article:7a46c9901e404d679b9184b028c319df2021-12-05T14:10:58ZMultiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy2191-909710.1515/ntrev-2021-0110https://doaj.org/article/7a46c9901e404d679b9184b028c319df2021-11-01T00:00:00Zhttps://doi.org/10.1515/ntrev-2021-0110https://doaj.org/toc/2191-9097To date, the use of nanocarriers has been developed in various fields, especially in cancer treatment. Graphene oxide (GO) is a novel drug delivery system that eagerly attracts the attention of many researchers due to its unique features. For the first time, a biocompatible AS1411 APT-GO-COOH was synthesized for the co-delivery of chemotherapeutics and herbal drugs. Here, a human gastric adenocarcinoma cell line (AGS) was targeted with aptamer-carboxylated graphene oxide (APT-CGO) containing anticancer drugs (curcumin (CUR) and doxorubicin (DOX)). The current study aimed to assess the anti-cancer effect of combination therapy, as well as target genes and proteins interfering in the development of gastric cancer. After attachment of APT to CGO, the drugs (CUR and DOX) were loaded on the carrier, establishing a co-delivery system. Then, physical characteristics, release profile, cytotoxicity assay, cellular uptake, expression rates of the genes (RB1, CDK2, AKT, and NF-KB) and proteins (RB1, CDK2), and the apoptosis rate were determined. The designed co-delivery system for the drugs (CUR and DOX) and APT showed a thermo- and pH-sensitive drug release behavior that successfully reduced the expression of CDK2, AKT, and NF-KB while it enhanced RB1 expression at the gene and protein levels. Also, APT-CGO-drugs were successfully targeted to the AGS cell line, leading to a highly inhibitory property against this cell line compared to CGO-drugs. It seems that the co-delivery of CUR and DOX along with APT as a targeting agent was more effective than CGO-drugs, suggesting a promising candidate for the treatment of gastric cancer. The results showed that this biofunctionalized nanocarrier could reduce the cytotoxicity of the drugs in normal cells and could increase efficiency.Yaghoubi FatemehNaghib Seyed MortezaMotlagh Najmeh Sadat HosseiniHaghiralsadat FatemeJaliani Hossein ZareiTofighi DavoodMoradi AliDe Gruyterarticlegraphene oxidecurcumindoxorubicinaptamercell culturedrug deliverynanoparticleTechnologyTChemical technologyTP1-1185Physical and theoretical chemistryQD450-801ENNanotechnology Reviews, Vol 10, Iss 1, Pp 1838-1852 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	graphene oxide curcumin doxorubicin aptamer cell culture drug delivery nanoparticle Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801
spellingShingle	graphene oxide curcumin doxorubicin aptamer cell culture drug delivery nanoparticle Technology T Chemical technology TP1-1185 Physical and theoretical chemistry QD450-801 Yaghoubi Fatemeh Naghib Seyed Morteza Motlagh Najmeh Sadat Hosseini Haghiralsadat Fateme Jaliani Hossein Zarei Tofighi Davood Moradi Ali Multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy
description	To date, the use of nanocarriers has been developed in various fields, especially in cancer treatment. Graphene oxide (GO) is a novel drug delivery system that eagerly attracts the attention of many researchers due to its unique features. For the first time, a biocompatible AS1411 APT-GO-COOH was synthesized for the co-delivery of chemotherapeutics and herbal drugs. Here, a human gastric adenocarcinoma cell line (AGS) was targeted with aptamer-carboxylated graphene oxide (APT-CGO) containing anticancer drugs (curcumin (CUR) and doxorubicin (DOX)). The current study aimed to assess the anti-cancer effect of combination therapy, as well as target genes and proteins interfering in the development of gastric cancer. After attachment of APT to CGO, the drugs (CUR and DOX) were loaded on the carrier, establishing a co-delivery system. Then, physical characteristics, release profile, cytotoxicity assay, cellular uptake, expression rates of the genes (RB1, CDK2, AKT, and NF-KB) and proteins (RB1, CDK2), and the apoptosis rate were determined. The designed co-delivery system for the drugs (CUR and DOX) and APT showed a thermo- and pH-sensitive drug release behavior that successfully reduced the expression of CDK2, AKT, and NF-KB while it enhanced RB1 expression at the gene and protein levels. Also, APT-CGO-drugs were successfully targeted to the AGS cell line, leading to a highly inhibitory property against this cell line compared to CGO-drugs. It seems that the co-delivery of CUR and DOX along with APT as a targeting agent was more effective than CGO-drugs, suggesting a promising candidate for the treatment of gastric cancer. The results showed that this biofunctionalized nanocarrier could reduce the cytotoxicity of the drugs in normal cells and could increase efficiency.
format	article
author	Yaghoubi Fatemeh Naghib Seyed Morteza Motlagh Najmeh Sadat Hosseini Haghiralsadat Fateme Jaliani Hossein Zarei Tofighi Davood Moradi Ali
author_facet	Yaghoubi Fatemeh Naghib Seyed Morteza Motlagh Najmeh Sadat Hosseini Haghiralsadat Fateme Jaliani Hossein Zarei Tofighi Davood Moradi Ali
author_sort	Yaghoubi Fatemeh
title	Multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy
title_short	Multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy
title_full	Multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy
title_fullStr	Multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy
title_full_unstemmed	Multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy
title_sort	multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy
publisher	De Gruyter
publishDate	2021
url	https://doaj.org/article/7a46c9901e404d679b9184b028c319df
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Multiresponsive carboxylated graphene oxide-grafted aptamer as a multifunctional nanocarrier for targeted delivery of chemotherapeutics and bioactive compounds in cancer therapy

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