Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif

The DIR2s RNA aptamer activates the fluorescence of cyanine dyes allowing detection of two well-resolved emission colors. Here authors solve the crystal structures of the apo and OTB-SO3 fluorophore-bound DIR2s and show how the fluorophore ligand is bound.

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Bibliographic Details
Main Authors:	Sandip A. Shelke, Yaming Shao, Artur Laski, Deepak Koirala, Benjamin P. Weissman, James R. Fuller, Xiaohong Tan, Tudor P. Constantin, Alan S. Waggoner, Marcel P. Bruchez, Bruce A. Armitage, Joseph A. Piccirilli
Format:	article
Language:	EN
Published:	Nature Portfolio 2018
Subjects:	Science Q
Online Access:	https://doaj.org/article/7a5169ba2e1440eb811c773399e43486
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spelling	oai:doaj.org-article:7a5169ba2e1440eb811c773399e434862021-12-02T17:32:29ZStructural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif10.1038/s41467-018-06942-32041-1723https://doaj.org/article/7a5169ba2e1440eb811c773399e434862018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06942-3https://doaj.org/toc/2041-1723The DIR2s RNA aptamer activates the fluorescence of cyanine dyes allowing detection of two well-resolved emission colors. Here authors solve the crystal structures of the apo and OTB-SO3 fluorophore-bound DIR2s and show how the fluorophore ligand is bound.Sandip A. ShelkeYaming ShaoArtur LaskiDeepak KoiralaBenjamin P. WeissmanJames R. FullerXiaohong TanTudor P. ConstantinAlan S. WaggonerMarcel P. BruchezBruce A. ArmitageJoseph A. PiccirilliNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Sandip A. Shelke Yaming Shao Artur Laski Deepak Koirala Benjamin P. Weissman James R. Fuller Xiaohong Tan Tudor P. Constantin Alan S. Waggoner Marcel P. Bruchez Bruce A. Armitage Joseph A. Piccirilli Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
description	The DIR2s RNA aptamer activates the fluorescence of cyanine dyes allowing detection of two well-resolved emission colors. Here authors solve the crystal structures of the apo and OTB-SO3 fluorophore-bound DIR2s and show how the fluorophore ligand is bound.
format	article
author	Sandip A. Shelke Yaming Shao Artur Laski Deepak Koirala Benjamin P. Weissman James R. Fuller Xiaohong Tan Tudor P. Constantin Alan S. Waggoner Marcel P. Bruchez Bruce A. Armitage Joseph A. Piccirilli
author_facet	Sandip A. Shelke Yaming Shao Artur Laski Deepak Koirala Benjamin P. Weissman James R. Fuller Xiaohong Tan Tudor P. Constantin Alan S. Waggoner Marcel P. Bruchez Bruce A. Armitage Joseph A. Piccirilli
author_sort	Sandip A. Shelke
title	Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_short	Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_full	Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_fullStr	Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_full_unstemmed	Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_sort	structural basis for activation of fluorogenic dyes by an rna aptamer lacking a g-quadruplex motif
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/7a5169ba2e1440eb811c773399e43486
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Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif

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