Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif

The DIR2s RNA aptamer activates the fluorescence of cyanine dyes allowing detection of two well-resolved emission colors. Here authors solve the crystal structures of the apo and OTB-SO3 fluorophore-bound DIR2s and show how the fluorophore ligand is bound.

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Autores principales: Sandip A. Shelke, Yaming Shao, Artur Laski, Deepak Koirala, Benjamin P. Weissman, James R. Fuller, Xiaohong Tan, Tudor P. Constantin, Alan S. Waggoner, Marcel P. Bruchez, Bruce A. Armitage, Joseph A. Piccirilli
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Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/7a5169ba2e1440eb811c773399e43486
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spelling oai:doaj.org-article:7a5169ba2e1440eb811c773399e434862021-12-02T17:32:29ZStructural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif10.1038/s41467-018-06942-32041-1723https://doaj.org/article/7a5169ba2e1440eb811c773399e434862018-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-06942-3https://doaj.org/toc/2041-1723The DIR2s RNA aptamer activates the fluorescence of cyanine dyes allowing detection of two well-resolved emission colors. Here authors solve the crystal structures of the apo and OTB-SO3 fluorophore-bound DIR2s and show how the fluorophore ligand is bound.Sandip A. ShelkeYaming ShaoArtur LaskiDeepak KoiralaBenjamin P. WeissmanJames R. FullerXiaohong TanTudor P. ConstantinAlan S. WaggonerMarcel P. BruchezBruce A. ArmitageJoseph A. PiccirilliNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-10 (2018)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Sandip A. Shelke
Yaming Shao
Artur Laski
Deepak Koirala
Benjamin P. Weissman
James R. Fuller
Xiaohong Tan
Tudor P. Constantin
Alan S. Waggoner
Marcel P. Bruchez
Bruce A. Armitage
Joseph A. Piccirilli
Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
description The DIR2s RNA aptamer activates the fluorescence of cyanine dyes allowing detection of two well-resolved emission colors. Here authors solve the crystal structures of the apo and OTB-SO3 fluorophore-bound DIR2s and show how the fluorophore ligand is bound.
format article
author Sandip A. Shelke
Yaming Shao
Artur Laski
Deepak Koirala
Benjamin P. Weissman
James R. Fuller
Xiaohong Tan
Tudor P. Constantin
Alan S. Waggoner
Marcel P. Bruchez
Bruce A. Armitage
Joseph A. Piccirilli
author_facet Sandip A. Shelke
Yaming Shao
Artur Laski
Deepak Koirala
Benjamin P. Weissman
James R. Fuller
Xiaohong Tan
Tudor P. Constantin
Alan S. Waggoner
Marcel P. Bruchez
Bruce A. Armitage
Joseph A. Piccirilli
author_sort Sandip A. Shelke
title Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_short Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_full Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_fullStr Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_full_unstemmed Structural basis for activation of fluorogenic dyes by an RNA aptamer lacking a G-quadruplex motif
title_sort structural basis for activation of fluorogenic dyes by an rna aptamer lacking a g-quadruplex motif
publisher Nature Portfolio
publishDate 2018
url https://doaj.org/article/7a5169ba2e1440eb811c773399e43486
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