Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate

With the current study, we aimed to determine the characteristics and calcium absorption capacity of egg white peptide–calcium complex (EWP-Ca) and determine the effect of sterilization on EWP-Ca to study the possibility of EWP-Ca as a new potential calcium supplement. The results of SEM and EDS sho...

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Autores principales:	Zhijie Bao, Penglin Zhang, Na Sun, Songyi Lin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	egg white peptide–calcium chelate characterization thermal stability calcium supplement Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/7a62bc28d8f24f4281f5fa5bcc865cb2
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spelling	oai:doaj.org-article:7a62bc28d8f24f4281f5fa5bcc865cb22021-11-25T17:32:42ZElucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate10.3390/foods101125652304-8158https://doaj.org/article/7a62bc28d8f24f4281f5fa5bcc865cb22021-10-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2565https://doaj.org/toc/2304-8158With the current study, we aimed to determine the characteristics and calcium absorption capacity of egg white peptide–calcium complex (EWP-Ca) and determine the effect of sterilization on EWP-Ca to study the possibility of EWP-Ca as a new potential calcium supplement. The results of SEM and EDS showed a high calcium chelating ability between EWP and calcium, and the structure of EWP-Ca was clustered spherical particles due its combination with calcium. The FTIR and Raman spectrum results showed that EWP could chelate with calcium by carboxyl, phosphate, and amino groups, and peptide bonds may also participate in peptide–calcium binding. Moreover, the calcium absorption of EWP-Ca measured by the intestinal everted sac model in rats was 32.38 ± 6.83 μg/mL, significantly higher than the sample with CaCl<sub>2,</sub> and the mixture of EWP and Ca (<i>p <</i> 0.05) revealed appropriate calcium absorption capacity. The fluorescence spectra and CD spectra showed that sterilization caused a decrease in the content of α-helix and β-sheet and a significant increase in β-turn (<i>p <</i> 0.05). Sterilization changed the EWP-Ca structure and decreased its stability; the calcium-binding capacity of EWP-Ca after sterilization was decreased to 41.19% (<i>p <</i> 0.05). Overall, these findings showed that EWP could bind with calcium, form a peptide–calcium chelate, and serve as novel carriers for calcium supplements.Zhijie BaoPenglin ZhangNa SunSongyi LinMDPI AGarticleegg whitepeptide–calcium chelatecharacterizationthermal stabilitycalcium supplementChemical technologyTP1-1185ENFoods, Vol 10, Iss 2565, p 2565 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	egg white peptide–calcium chelate characterization thermal stability calcium supplement Chemical technology TP1-1185
spellingShingle	egg white peptide–calcium chelate characterization thermal stability calcium supplement Chemical technology TP1-1185 Zhijie Bao Penglin Zhang Na Sun Songyi Lin Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate
description	With the current study, we aimed to determine the characteristics and calcium absorption capacity of egg white peptide–calcium complex (EWP-Ca) and determine the effect of sterilization on EWP-Ca to study the possibility of EWP-Ca as a new potential calcium supplement. The results of SEM and EDS showed a high calcium chelating ability between EWP and calcium, and the structure of EWP-Ca was clustered spherical particles due its combination with calcium. The FTIR and Raman spectrum results showed that EWP could chelate with calcium by carboxyl, phosphate, and amino groups, and peptide bonds may also participate in peptide–calcium binding. Moreover, the calcium absorption of EWP-Ca measured by the intestinal everted sac model in rats was 32.38 ± 6.83 μg/mL, significantly higher than the sample with CaCl<sub>2,</sub> and the mixture of EWP and Ca (<i>p <</i> 0.05) revealed appropriate calcium absorption capacity. The fluorescence spectra and CD spectra showed that sterilization caused a decrease in the content of α-helix and β-sheet and a significant increase in β-turn (<i>p <</i> 0.05). Sterilization changed the EWP-Ca structure and decreased its stability; the calcium-binding capacity of EWP-Ca after sterilization was decreased to 41.19% (<i>p <</i> 0.05). Overall, these findings showed that EWP could bind with calcium, form a peptide–calcium chelate, and serve as novel carriers for calcium supplements.
format	article
author	Zhijie Bao Penglin Zhang Na Sun Songyi Lin
author_facet	Zhijie Bao Penglin Zhang Na Sun Songyi Lin
author_sort	Zhijie Bao
title	Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate
title_short	Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate
title_full	Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate
title_fullStr	Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate
title_full_unstemmed	Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate
title_sort	elucidating the calcium-binding site, absorption activities, and thermal stability of egg white peptide–calcium chelate
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7a62bc28d8f24f4281f5fa5bcc865cb2
work_keys_str_mv	AT zhijiebao elucidatingthecalciumbindingsiteabsorptionactivitiesandthermalstabilityofeggwhitepeptidecalciumchelate AT penglinzhang elucidatingthecalciumbindingsiteabsorptionactivitiesandthermalstabilityofeggwhitepeptidecalciumchelate AT nasun elucidatingthecalciumbindingsiteabsorptionactivitiesandthermalstabilityofeggwhitepeptidecalciumchelate AT songyilin elucidatingthecalciumbindingsiteabsorptionactivitiesandthermalstabilityofeggwhitepeptidecalciumchelate
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Elucidating the Calcium-Binding Site, Absorption Activities, and Thermal Stability of Egg White Peptide–Calcium Chelate

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