Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.

Autoinducer-2 (AI-2) molecules are one class of signalling molecules involved in gene regulation dependent on population density in a mechanism commonly referred to as quorum sensing (QS). AI-2 is produced by the methylthioadenosine/S-adenosyl-homocysteine nucleosidase LuxS. In the present study, we...

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Autores principales:	Zhongke Sun, Xiang He, Vincenzo F Brancaccio, Jing Yuan, Christian U Riedel
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a6425b034b14f69b39818cf0ad9d853
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spelling	oai:doaj.org-article:7a6425b034b14f69b39818cf0ad9d8532021-11-18T08:33:35ZBifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.1932-620310.1371/journal.pone.0088260https://doaj.org/article/7a6425b034b14f69b39818cf0ad9d8532014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24505453/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Autoinducer-2 (AI-2) molecules are one class of signalling molecules involved in gene regulation dependent on population density in a mechanism commonly referred to as quorum sensing (QS). AI-2 is produced by the methylthioadenosine/S-adenosyl-homocysteine nucleosidase LuxS. In the present study, we characterise the function of bifidobacterial LuxS proteins to address the question whether these economically important bacteria are able to perform QS communication. All publically available genome sequences of bifidobacteria harbour putative luxS genes. The deduced amino acid sequences are well conserved in the genus and show good homology to the LuxS protein of the prototypical AI-2 producer Vibrio harveyi. The luxS genes of three bifidobacterial strains were successfully expressed in AI-2-negative Escherichia coli DH5α. Supernatants of these recombinant E. coli strains contained significant AI-2 activity. In initial experiments, we failed to detect AI-2 activity in supernatants of bifidobacteria grown in MRSc. High concentration of glucose as well as acidic pH had strong inhibitory effects on AI-2 activity. AI-2 activity could be detected when lower volumes of supernatants were used in the assay. Homologous overexpression of luxS in Bifidobacterium longum NCC2705 increased AI-2 levels in the supernatant. Furthermore, over-expression of luxS or supplementation with AI-2-containing supernatants enhanced biofilm formation of B. longum NCC2705. Collectively, these results suggest that bifidobacteria indeed harbour functional luxS genes that are involved in the production of AI-2-like molecules. To the best of our knowledge, this represents the first report on AI-2 activity produced by bifidobacteria. Self-produced AI-2 activity as well as AI-2-like molecules of other bacteria of the intestinal tract may have a regulatory function in biofilm formation and host colonization by bifidobacteria.Zhongke SunXiang HeVincenzo F BrancaccioJing YuanChristian U RiedelPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88260 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhongke Sun Xiang He Vincenzo F Brancaccio Jing Yuan Christian U Riedel Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.
description	Autoinducer-2 (AI-2) molecules are one class of signalling molecules involved in gene regulation dependent on population density in a mechanism commonly referred to as quorum sensing (QS). AI-2 is produced by the methylthioadenosine/S-adenosyl-homocysteine nucleosidase LuxS. In the present study, we characterise the function of bifidobacterial LuxS proteins to address the question whether these economically important bacteria are able to perform QS communication. All publically available genome sequences of bifidobacteria harbour putative luxS genes. The deduced amino acid sequences are well conserved in the genus and show good homology to the LuxS protein of the prototypical AI-2 producer Vibrio harveyi. The luxS genes of three bifidobacterial strains were successfully expressed in AI-2-negative Escherichia coli DH5α. Supernatants of these recombinant E. coli strains contained significant AI-2 activity. In initial experiments, we failed to detect AI-2 activity in supernatants of bifidobacteria grown in MRSc. High concentration of glucose as well as acidic pH had strong inhibitory effects on AI-2 activity. AI-2 activity could be detected when lower volumes of supernatants were used in the assay. Homologous overexpression of luxS in Bifidobacterium longum NCC2705 increased AI-2 levels in the supernatant. Furthermore, over-expression of luxS or supplementation with AI-2-containing supernatants enhanced biofilm formation of B. longum NCC2705. Collectively, these results suggest that bifidobacteria indeed harbour functional luxS genes that are involved in the production of AI-2-like molecules. To the best of our knowledge, this represents the first report on AI-2 activity produced by bifidobacteria. Self-produced AI-2 activity as well as AI-2-like molecules of other bacteria of the intestinal tract may have a regulatory function in biofilm formation and host colonization by bifidobacteria.
format	article
author	Zhongke Sun Xiang He Vincenzo F Brancaccio Jing Yuan Christian U Riedel
author_facet	Zhongke Sun Xiang He Vincenzo F Brancaccio Jing Yuan Christian U Riedel
author_sort	Zhongke Sun
title	Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.
title_short	Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.
title_full	Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.
title_fullStr	Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.
title_full_unstemmed	Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.
title_sort	bifidobacteria exhibit luxs-dependent autoinducer 2 activity and biofilm formation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7a6425b034b14f69b39818cf0ad9d853
work_keys_str_mv	AT zhongkesun bifidobacteriaexhibitluxsdependentautoinducer2activityandbiofilmformation AT xianghe bifidobacteriaexhibitluxsdependentautoinducer2activityandbiofilmformation AT vincenzofbrancaccio bifidobacteriaexhibitluxsdependentautoinducer2activityandbiofilmformation AT jingyuan bifidobacteriaexhibitluxsdependentautoinducer2activityandbiofilmformation AT christianuriedel bifidobacteriaexhibitluxsdependentautoinducer2activityandbiofilmformation
_version_	1718421630101749760

Bifidobacteria exhibit LuxS-dependent autoinducer 2 activity and biofilm formation.

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