Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi

Abstract Fungal-fungal interaction often leads to the change in metabolite profile of both the interacting fungus which may have potential implication in industry or agriculture. In the present study, we performed two sets of fungal-fungal interaction—Trametes coccinea (F3) with Leiotrametes lactine...

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Autores principales:	Samim Dullah, Dibya Jyoti Hazarika, Gunajit Goswami, Tanushree Borgohain, Alokesh Ghosh, Madhumita Barooah, Ashok Bhattacharyya, Robin Chandra Boro
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Hyphal interactions Laccase Melanin NMR spectroscopy Superoxide dismutase ROS Botany QK1-989
Acceso en línea:	https://doaj.org/article/7a772e9470ef432b9f79c67e721e3953
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spelling	oai:doaj.org-article:7a772e9470ef432b9f79c67e721e39532021-11-14T12:15:55ZMelanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi10.1186/s43008-021-00082-y2210-6359https://doaj.org/article/7a772e9470ef432b9f79c67e721e39532021-11-01T00:00:00Zhttps://doi.org/10.1186/s43008-021-00082-yhttps://doaj.org/toc/2210-6359Abstract Fungal-fungal interaction often leads to the change in metabolite profile of both the interacting fungus which may have potential implication in industry or agriculture. In the present study, we performed two sets of fungal-fungal interaction—Trametes coccinea (F3) with Leiotrametes lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) to understand the changes in the metabolite profile during the interaction process and how this process impacts the hyphal/mycelial morphology of the participating fungi. The metabolites produced during interaction of T. coccinea (F3) with L. lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) was analysed through liquid chromatography coupled to mass spectroscopy (LC-MS). Most of the metabolites secreted or produced during interaction are associated with defensive response. Further, visualization with scanning electron microscopy revealed that interaction between the tested fungi led to the changes in the hyphal morphology. The bipartite fungal interaction resulted in the production of a dark brown colour pigment—melanin as confirmed by the LC-MS, FTIR and NMR analysis. Moreover, the fungal–fungal interaction also led to increase in the production of laccase, a group of multicopper oxidases involved in detoxification of toxic compounds. Further, increased activity of superoxide dismutase, an enzyme that catalyzes the dismutation of the superoxide anion to hydrogen peroxide was also recorded during fungal–fungal interaction. Quantitative real-time PCR revealed upregulation of lcc1 (encoding a laccase enzyme) and few other stress related genes of T. versicolor during its hyphal interaction with T. coccinea, suggesting a direct correlation between laccase production and melanin production.Samim DullahDibya Jyoti HazarikaGunajit GoswamiTanushree BorgohainAlokesh GhoshMadhumita BarooahAshok BhattacharyyaRobin Chandra BoroBMCarticleHyphal interactionsLaccaseMelaninNMR spectroscopySuperoxide dismutaseROSBotanyQK1-989ENIMA Fungus, Vol 12, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Hyphal interactions Laccase Melanin NMR spectroscopy Superoxide dismutase ROS Botany QK1-989
spellingShingle	Hyphal interactions Laccase Melanin NMR spectroscopy Superoxide dismutase ROS Botany QK1-989 Samim Dullah Dibya Jyoti Hazarika Gunajit Goswami Tanushree Borgohain Alokesh Ghosh Madhumita Barooah Ashok Bhattacharyya Robin Chandra Boro Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
description	Abstract Fungal-fungal interaction often leads to the change in metabolite profile of both the interacting fungus which may have potential implication in industry or agriculture. In the present study, we performed two sets of fungal-fungal interaction—Trametes coccinea (F3) with Leiotrametes lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) to understand the changes in the metabolite profile during the interaction process and how this process impacts the hyphal/mycelial morphology of the participating fungi. The metabolites produced during interaction of T. coccinea (F3) with L. lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) was analysed through liquid chromatography coupled to mass spectroscopy (LC-MS). Most of the metabolites secreted or produced during interaction are associated with defensive response. Further, visualization with scanning electron microscopy revealed that interaction between the tested fungi led to the changes in the hyphal morphology. The bipartite fungal interaction resulted in the production of a dark brown colour pigment—melanin as confirmed by the LC-MS, FTIR and NMR analysis. Moreover, the fungal–fungal interaction also led to increase in the production of laccase, a group of multicopper oxidases involved in detoxification of toxic compounds. Further, increased activity of superoxide dismutase, an enzyme that catalyzes the dismutation of the superoxide anion to hydrogen peroxide was also recorded during fungal–fungal interaction. Quantitative real-time PCR revealed upregulation of lcc1 (encoding a laccase enzyme) and few other stress related genes of T. versicolor during its hyphal interaction with T. coccinea, suggesting a direct correlation between laccase production and melanin production.
format	article
author	Samim Dullah Dibya Jyoti Hazarika Gunajit Goswami Tanushree Borgohain Alokesh Ghosh Madhumita Barooah Ashok Bhattacharyya Robin Chandra Boro
author_facet	Samim Dullah Dibya Jyoti Hazarika Gunajit Goswami Tanushree Borgohain Alokesh Ghosh Madhumita Barooah Ashok Bhattacharyya Robin Chandra Boro
author_sort	Samim Dullah
title	Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
title_short	Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
title_full	Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
title_fullStr	Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
title_full_unstemmed	Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
title_sort	melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi
publisher	BMC
publishDate	2021
url	https://doaj.org/article/7a772e9470ef432b9f79c67e721e3953
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Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi

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