Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells

Abstract Calcium signalling through NMDA-type glutamate receptors (NMDARs) plays a key role in synaptic plasticity in the central nervous system (CNS). NMDAR expression has also been detected in other tissues and aberrant glutamate signalling has been linked to cancer; however, the significance of N...

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Autor principal:	Oleg O. Glebov
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a7d9933af4d4360b9576e2bc5dff1a0
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spelling	oai:doaj.org-article:7a7d9933af4d4360b9576e2bc5dff1a02021-12-02T17:52:24ZTonic NMDA receptor signalling shapes endosomal organisation in mammalian cells10.1038/s41598-020-66071-02045-2322https://doaj.org/article/7a7d9933af4d4360b9576e2bc5dff1a02020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66071-0https://doaj.org/toc/2045-2322Abstract Calcium signalling through NMDA-type glutamate receptors (NMDARs) plays a key role in synaptic plasticity in the central nervous system (CNS). NMDAR expression has also been detected in other tissues and aberrant glutamate signalling has been linked to cancer; however, the significance of NMDAR function outside of the CNS remains unclear. Here, I show that removal of extracellular calcium rapidly decreases the size of early endosomes in primary human fibroblasts. This effect can be mimicked by blockade of NMDA-type glutamate receptors but not voltage-gated calcium channels (VGCCs), and can also be observed in primary hippocampal neurons and Jurkat T cells. Conversely, in a breast cancer cell line MDA-MB-231 NMDAR blockade results in an increase in endosomal size and decrease in number. These findings reveal that calcium signalling via glutamate receptors controls the structure of the endosomal system and suggest that aberrations in NMDAR-regulated membrane trafficking may be associated with cancer.Oleg O. GlebovNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Oleg O. Glebov Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells
description	Abstract Calcium signalling through NMDA-type glutamate receptors (NMDARs) plays a key role in synaptic plasticity in the central nervous system (CNS). NMDAR expression has also been detected in other tissues and aberrant glutamate signalling has been linked to cancer; however, the significance of NMDAR function outside of the CNS remains unclear. Here, I show that removal of extracellular calcium rapidly decreases the size of early endosomes in primary human fibroblasts. This effect can be mimicked by blockade of NMDA-type glutamate receptors but not voltage-gated calcium channels (VGCCs), and can also be observed in primary hippocampal neurons and Jurkat T cells. Conversely, in a breast cancer cell line MDA-MB-231 NMDAR blockade results in an increase in endosomal size and decrease in number. These findings reveal that calcium signalling via glutamate receptors controls the structure of the endosomal system and suggest that aberrations in NMDAR-regulated membrane trafficking may be associated with cancer.
format	article
author	Oleg O. Glebov
author_facet	Oleg O. Glebov
author_sort	Oleg O. Glebov
title	Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells
title_short	Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells
title_full	Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells
title_fullStr	Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells
title_full_unstemmed	Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells
title_sort	tonic nmda receptor signalling shapes endosomal organisation in mammalian cells
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7a7d9933af4d4360b9576e2bc5dff1a0
work_keys_str_mv	AT olegoglebov tonicnmdareceptorsignallingshapesendosomalorganisationinmammaliancells
_version_	1718379234141929472

Tonic NMDA receptor signalling shapes endosomal organisation in mammalian cells

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