Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA

Abstract Cell therapy limits ischemic injury following myocardial infarction (MI) by preventing cell death, modulating the immune response, and promoting tissue regeneration. The therapeutic efficacy of cardiosphere-derived cells (CDCs) and mesenchymal stem cells (MSCs) is associated with extracellu...

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Autores principales:	Ann-Sophie Walravens, Sasha Smolgovsky, Liang Li, Lauren Kelly, Travis Antes, Kiel Peck, Tanner Quon, Ahmed Ibrahim, Eduardo Marbán, Benjamin Berman, Linda Marbán, Luis R.-Borlado, Geoffrey de Couto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a83485ee04f42dbafb63c973bb42fd4
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spelling	oai:doaj.org-article:7a83485ee04f42dbafb63c973bb42fd42021-12-02T16:45:06ZMechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA10.1038/s41598-021-87939-92045-2322https://doaj.org/article/7a83485ee04f42dbafb63c973bb42fd42021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87939-9https://doaj.org/toc/2045-2322Abstract Cell therapy limits ischemic injury following myocardial infarction (MI) by preventing cell death, modulating the immune response, and promoting tissue regeneration. The therapeutic efficacy of cardiosphere-derived cells (CDCs) and mesenchymal stem cells (MSCs) is associated with extracellular vesicle (EV) release. Prior head-to-head comparisons have shown CDCs to be more effective than MSCs in MI models. Despite differences in cell origin, it is unclear why EVs from different adult stem cell populations elicit differences in therapeutic efficacy. Here, we compare EVs derived from multiple human MSC and CDC donors using diverse in vitro and in vivo assays. EV membrane protein and non-coding RNA composition are highly specific to the parent cell type; for example, miR-10b is enriched in MSC-EVs relative to CDC-EVs, while Y RNA fragments follow the opposite pattern. CDC-EVs enhance the Arg1/Nos2 ratio in macrophages in vitro and reduce MI size more than MSC-EVs and suppress inflammation during acute peritonitis in vivo. Thus, CDC-EVs are distinct from MSC-EVs, confer immunomodulation, and protect the host against ischemic myocardial injury and acute inflammation.Ann-Sophie WalravensSasha SmolgovskyLiang LiLauren KellyTravis AntesKiel PeckTanner QuonAhmed IbrahimEduardo MarbánBenjamin BermanLinda MarbánLuis R.-BorladoGeoffrey de CoutoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ann-Sophie Walravens Sasha Smolgovsky Liang Li Lauren Kelly Travis Antes Kiel Peck Tanner Quon Ahmed Ibrahim Eduardo Marbán Benjamin Berman Linda Marbán Luis R.-Borlado Geoffrey de Couto Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA
description	Abstract Cell therapy limits ischemic injury following myocardial infarction (MI) by preventing cell death, modulating the immune response, and promoting tissue regeneration. The therapeutic efficacy of cardiosphere-derived cells (CDCs) and mesenchymal stem cells (MSCs) is associated with extracellular vesicle (EV) release. Prior head-to-head comparisons have shown CDCs to be more effective than MSCs in MI models. Despite differences in cell origin, it is unclear why EVs from different adult stem cell populations elicit differences in therapeutic efficacy. Here, we compare EVs derived from multiple human MSC and CDC donors using diverse in vitro and in vivo assays. EV membrane protein and non-coding RNA composition are highly specific to the parent cell type; for example, miR-10b is enriched in MSC-EVs relative to CDC-EVs, while Y RNA fragments follow the opposite pattern. CDC-EVs enhance the Arg1/Nos2 ratio in macrophages in vitro and reduce MI size more than MSC-EVs and suppress inflammation during acute peritonitis in vivo. Thus, CDC-EVs are distinct from MSC-EVs, confer immunomodulation, and protect the host against ischemic myocardial injury and acute inflammation.
format	article
author	Ann-Sophie Walravens Sasha Smolgovsky Liang Li Lauren Kelly Travis Antes Kiel Peck Tanner Quon Ahmed Ibrahim Eduardo Marbán Benjamin Berman Linda Marbán Luis R.-Borlado Geoffrey de Couto
author_facet	Ann-Sophie Walravens Sasha Smolgovsky Liang Li Lauren Kelly Travis Antes Kiel Peck Tanner Quon Ahmed Ibrahim Eduardo Marbán Benjamin Berman Linda Marbán Luis R.-Borlado Geoffrey de Couto
author_sort	Ann-Sophie Walravens
title	Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA
title_short	Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA
title_full	Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA
title_fullStr	Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA
title_full_unstemmed	Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA
title_sort	mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding rna
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7a83485ee04f42dbafb63c973bb42fd4
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Mechanistic and therapeutic distinctions between cardiosphere-derived cell and mesenchymal stem cell extracellular vesicle non-coding RNA

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