Ananas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats

Oxidative stress is implicated in the pathophysiology of a plethora of diseases. In this research, pilot studies were carried out in the methanol extracts of the ten fruit peels to screen for antioxidant activity. Two fruit peels with the highest antioxidant activity were selected. The methanol peel...

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Autores principales:	Henrietta Chisom Unanma, Emeka Godwin Anaduaka, Nene Orizu Uchendu, Chidimma Pamela Ononiwu, Victor Nwadiogbu Ogugua
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Oxidative stress Hepatoprotective Carbon tetrachloride (CCl4) Fruit peels Histology Science Q
Acceso en línea:	https://doaj.org/article/7a88f704948444b099b64ea2f78d893a
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spelling	oai:doaj.org-article:7a88f704948444b099b64ea2f78d893a2021-11-22T04:28:07ZAnanas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats2468-227610.1016/j.sciaf.2021.e01026https://doaj.org/article/7a88f704948444b099b64ea2f78d893a2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2468227621003276https://doaj.org/toc/2468-2276Oxidative stress is implicated in the pathophysiology of a plethora of diseases. In this research, pilot studies were carried out in the methanol extracts of the ten fruit peels to screen for antioxidant activity. Two fruit peels with the highest antioxidant activity were selected. The methanol peel extracts of the selected two; Citrus sinensis (orange) and Ananas comosus (pineapple) were evaluated for their in vivo antioxidant and hepatoprotective properties. Twenty-eight adult Wistar rats of both sexes were used in this experiment and grouped into seven of four rats each. Group 1 and 2 served as normal and positive controls. Groups 3 to 7 were pre-treated with Silymarin (100 mg/kg), A. comosus methanol peel extract (ACMPE) 250 mg/kg and 500 mg/kg C. sinensis methanol peel extract (CSMPE) 250 mg/kg and 500 mg/kg respectively for seven days before intraperitoneally inducing oxidative stress with carbon tetrachloride (CCl4) on day eight. Serum analysis of biochemical parameters and histological examination of the liver was carried out using standard methods. The DPPH assay showed that the fruit peel of C. sinensis had the highest antioxidant concentration followed by that of A. comosus. Acute toxicity test (LD50) showed that ACMPE and CSMPE were tolerable at 5000 mg/kg. Phytochemical analysis of the two fruit peels extracts revealed the presence of flavonoids, alkaloids, glycosides, tannins, and acidic compounds. Administration of the extracts significantly elevated serum enzymatic levels of SOD and CAT in a dose-dependent manner. Additionally, the extracts substantially normalized altered MDA, ALT, AST, total protein, and total bilirubin levels. The lipid profile of the animals was greatly improved and histopathological studies confirmed the biochemical observations. The results of this study demonstrate that ACMPE and CSMPE possess antioxidant and hepatoprotective activity against CCl4-induced oxidative stress in experimental animals.Henrietta Chisom UnanmaEmeka Godwin AnaduakaNene Orizu UchenduChidimma Pamela OnoniwuVictor Nwadiogbu OguguaElsevierarticleOxidative stressHepatoprotectiveCarbon tetrachloride (CCl4)Fruit peelsHistologyScienceQENScientific African, Vol 14, Iss , Pp e01026- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Oxidative stress Hepatoprotective Carbon tetrachloride (CCl4) Fruit peels Histology Science Q
spellingShingle	Oxidative stress Hepatoprotective Carbon tetrachloride (CCl4) Fruit peels Histology Science Q Henrietta Chisom Unanma Emeka Godwin Anaduaka Nene Orizu Uchendu Chidimma Pamela Ononiwu Victor Nwadiogbu Ogugua Ananas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats
description	Oxidative stress is implicated in the pathophysiology of a plethora of diseases. In this research, pilot studies were carried out in the methanol extracts of the ten fruit peels to screen for antioxidant activity. Two fruit peels with the highest antioxidant activity were selected. The methanol peel extracts of the selected two; Citrus sinensis (orange) and Ananas comosus (pineapple) were evaluated for their in vivo antioxidant and hepatoprotective properties. Twenty-eight adult Wistar rats of both sexes were used in this experiment and grouped into seven of four rats each. Group 1 and 2 served as normal and positive controls. Groups 3 to 7 were pre-treated with Silymarin (100 mg/kg), A. comosus methanol peel extract (ACMPE) 250 mg/kg and 500 mg/kg C. sinensis methanol peel extract (CSMPE) 250 mg/kg and 500 mg/kg respectively for seven days before intraperitoneally inducing oxidative stress with carbon tetrachloride (CCl4) on day eight. Serum analysis of biochemical parameters and histological examination of the liver was carried out using standard methods. The DPPH assay showed that the fruit peel of C. sinensis had the highest antioxidant concentration followed by that of A. comosus. Acute toxicity test (LD50) showed that ACMPE and CSMPE were tolerable at 5000 mg/kg. Phytochemical analysis of the two fruit peels extracts revealed the presence of flavonoids, alkaloids, glycosides, tannins, and acidic compounds. Administration of the extracts significantly elevated serum enzymatic levels of SOD and CAT in a dose-dependent manner. Additionally, the extracts substantially normalized altered MDA, ALT, AST, total protein, and total bilirubin levels. The lipid profile of the animals was greatly improved and histopathological studies confirmed the biochemical observations. The results of this study demonstrate that ACMPE and CSMPE possess antioxidant and hepatoprotective activity against CCl4-induced oxidative stress in experimental animals.
format	article
author	Henrietta Chisom Unanma Emeka Godwin Anaduaka Nene Orizu Uchendu Chidimma Pamela Ononiwu Victor Nwadiogbu Ogugua
author_facet	Henrietta Chisom Unanma Emeka Godwin Anaduaka Nene Orizu Uchendu Chidimma Pamela Ononiwu Victor Nwadiogbu Ogugua
author_sort	Henrietta Chisom Unanma
title	Ananas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats
title_short	Ananas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats
title_full	Ananas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats
title_fullStr	Ananas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats
title_full_unstemmed	Ananas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats
title_sort	ananas comosus and citrus sinensis peels ameliorate ccl4-induced liver injury in wistar rats
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7a88f704948444b099b64ea2f78d893a
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Ananas comosus and Citrus sinensis peels ameliorate CCl4-induced liver injury in Wistar rats

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