Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate

Candida glabrata is among the most prevalent causes of candidiasis. Unlike Candida albicans, it is not capable of changing morphology between yeast and hyphal forms but instead has developed other virulence factors. An important feature is its unprecedented large repertoire of predicted cell wall ad...

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Autores principales:	Jordan Fernández-Pereira, María Alvarado, Emilia Gómez-Molero, Henk L. Dekker, María Teresa Blázquez-Muñoz, Elena Eraso, Oliver Bader, Piet W. J. de Groot
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	adhesion GPI protein cell wall protein host-pathogen interactions Candida glabrata candidiasis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7a928480f0e14e1abba0c1c1562af535
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spelling	oai:doaj.org-article:7a928480f0e14e1abba0c1c1562af5352021-11-15T05:01:35ZCharacterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate2235-298810.3389/fcimb.2021.790465https://doaj.org/article/7a928480f0e14e1abba0c1c1562af5352021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcimb.2021.790465/fullhttps://doaj.org/toc/2235-2988Candida glabrata is among the most prevalent causes of candidiasis. Unlike Candida albicans, it is not capable of changing morphology between yeast and hyphal forms but instead has developed other virulence factors. An important feature is its unprecedented large repertoire of predicted cell wall adhesins, which are thought to enable adherence to a variety of surfaces under different conditions. Here, we analyzed the wall proteome of PEU1221, a high biofilm-forming clinical strain isolated from an infected central venous catheter, under biofilm-forming conditions. This isolate shows increased incorporation of putative adhesins, including eight proteins that were not detected in walls of reference strain ATCC 2001, and of which Epa22, Awp14, and Awp2e were identified for the first time. The proteomics data suggest that cluster III adhesin Awp14 is relatively abundant in PEU1221. Phenotypic studies with awp14Δ deletion mutants showed that Awp14 is not responsible for the high biofilm formation of PEU1221 onto polystyrene. However, awp14Δ mutant cells in PEU1221 background showed a slightly diminished binding to chitin and seemed to sediment slightly slower than the parental strain suggesting implication in fungal cell-cell interactions. By structural modeling, we further demonstrate similarity between the ligand-binding domains of cluster III adhesin Awp14 and those of cluster V and VI adhesins. In conclusion, our work confirms the increased incorporation of putative adhesins, such as Awp14, in high biofilm-forming isolates, and contributes to decipher the precise role of these proteins in the establishment of C. glabrata infections.Jordan Fernández-PereiraMaría AlvaradoEmilia Gómez-MoleroEmilia Gómez-MoleroHenk L. DekkerMaría Teresa Blázquez-MuñozElena ErasoOliver BaderPiet W. J. de GrootFrontiers Media S.A.articleadhesionGPI proteincell wall proteinhost-pathogen interactionsCandida glabratacandidiasisMicrobiologyQR1-502ENFrontiers in Cellular and Infection Microbiology, Vol 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adhesion GPI protein cell wall protein host-pathogen interactions Candida glabrata candidiasis Microbiology QR1-502
spellingShingle	adhesion GPI protein cell wall protein host-pathogen interactions Candida glabrata candidiasis Microbiology QR1-502 Jordan Fernández-Pereira María Alvarado Emilia Gómez-Molero Emilia Gómez-Molero Henk L. Dekker María Teresa Blázquez-Muñoz Elena Eraso Oliver Bader Piet W. J. de Groot Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate
description	Candida glabrata is among the most prevalent causes of candidiasis. Unlike Candida albicans, it is not capable of changing morphology between yeast and hyphal forms but instead has developed other virulence factors. An important feature is its unprecedented large repertoire of predicted cell wall adhesins, which are thought to enable adherence to a variety of surfaces under different conditions. Here, we analyzed the wall proteome of PEU1221, a high biofilm-forming clinical strain isolated from an infected central venous catheter, under biofilm-forming conditions. This isolate shows increased incorporation of putative adhesins, including eight proteins that were not detected in walls of reference strain ATCC 2001, and of which Epa22, Awp14, and Awp2e were identified for the first time. The proteomics data suggest that cluster III adhesin Awp14 is relatively abundant in PEU1221. Phenotypic studies with awp14Δ deletion mutants showed that Awp14 is not responsible for the high biofilm formation of PEU1221 onto polystyrene. However, awp14Δ mutant cells in PEU1221 background showed a slightly diminished binding to chitin and seemed to sediment slightly slower than the parental strain suggesting implication in fungal cell-cell interactions. By structural modeling, we further demonstrate similarity between the ligand-binding domains of cluster III adhesin Awp14 and those of cluster V and VI adhesins. In conclusion, our work confirms the increased incorporation of putative adhesins, such as Awp14, in high biofilm-forming isolates, and contributes to decipher the precise role of these proteins in the establishment of C. glabrata infections.
format	article
author	Jordan Fernández-Pereira María Alvarado Emilia Gómez-Molero Emilia Gómez-Molero Henk L. Dekker María Teresa Blázquez-Muñoz Elena Eraso Oliver Bader Piet W. J. de Groot
author_facet	Jordan Fernández-Pereira María Alvarado Emilia Gómez-Molero Emilia Gómez-Molero Henk L. Dekker María Teresa Blázquez-Muñoz Elena Eraso Oliver Bader Piet W. J. de Groot
author_sort	Jordan Fernández-Pereira
title	Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate
title_short	Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate
title_full	Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate
title_fullStr	Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate
title_full_unstemmed	Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate
title_sort	characterization of awp14, a novel cluster iii adhesin identified in a high biofilm-forming candida glabrata isolate
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/7a928480f0e14e1abba0c1c1562af535
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Characterization of Awp14, A Novel Cluster III Adhesin Identified in a High Biofilm-Forming Candida glabrata Isolate

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