Platelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.

<h4>Background & aims</h4>Among the multiplicity of factors involved in rising incidence of hepatocellular carcinoma (HCC)-the second deadliest cancer, late diagnosis of early-stage HCC nodules originating from late-stage cirrhotic nodules is the most crucial. In recent years, Tumor-...

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Autores principales:	Walifa Waqar, Sidra Asghar, Sobia Manzoor
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a92a7447fe74c5dbce503d3d2daf94c
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spelling	oai:doaj.org-article:7a92a7447fe74c5dbce503d3d2daf94c2021-12-02T20:08:42ZPlatelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.1932-620310.1371/journal.pone.0256739https://doaj.org/article/7a92a7447fe74c5dbce503d3d2daf94c2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0256739https://doaj.org/toc/1932-6203<h4>Background & aims</h4>Among the multiplicity of factors involved in rising incidence of hepatocellular carcinoma (HCC)-the second deadliest cancer, late diagnosis of early-stage HCC nodules originating from late-stage cirrhotic nodules is the most crucial. In recent years, Tumor-educated platelets (TEPs) have emerged as a strong multimodal tool to be used in liquid-biopsy of cancers because of changes in their mRNA content. This study assessed the reliability of selected mRNA repertoire of platelets as biomarkers to differentiate early HCC from late-stage cirrhotic nodules.<h4>Methods</h4>Quantitative real time PCR (qRT-PCR) was used to evaluate expression levels of selected platelets-specific mRNA between HCC patients compared to cirrhosis patients. ROC curve analysis assessed the sensitivity and specificity of the biomarkers.<h4>Results</h4>RhoA, CTNNB1 and SPINK1 showed a significant 3.3-, 3.2- and 3.18-folds upregulation, respectively, in HCC patients compared to cirrhosis patients while IFITM3 and SERPIND1 presented a 2.24-fold change. Strikingly, CD41+ platelets also demonstrated a marked difference of expression in HCC and cirrhosis groups.<h4>Conclusions</h4>Our study reports liquid biopsy-based platelets mRNA signature for early diagnosis of HCC from underlying cirrhotic nodules. Moreover, differential expression of CD41+ platelets in two groups provides new insights into a probable link between CD41 expression on platelets with the progression of cirrhosis to HCC.Walifa WaqarSidra AsgharSobia ManzoorPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0256739 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Walifa Waqar Sidra Asghar Sobia Manzoor Platelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.
description	<h4>Background & aims</h4>Among the multiplicity of factors involved in rising incidence of hepatocellular carcinoma (HCC)-the second deadliest cancer, late diagnosis of early-stage HCC nodules originating from late-stage cirrhotic nodules is the most crucial. In recent years, Tumor-educated platelets (TEPs) have emerged as a strong multimodal tool to be used in liquid-biopsy of cancers because of changes in their mRNA content. This study assessed the reliability of selected mRNA repertoire of platelets as biomarkers to differentiate early HCC from late-stage cirrhotic nodules.<h4>Methods</h4>Quantitative real time PCR (qRT-PCR) was used to evaluate expression levels of selected platelets-specific mRNA between HCC patients compared to cirrhosis patients. ROC curve analysis assessed the sensitivity and specificity of the biomarkers.<h4>Results</h4>RhoA, CTNNB1 and SPINK1 showed a significant 3.3-, 3.2- and 3.18-folds upregulation, respectively, in HCC patients compared to cirrhosis patients while IFITM3 and SERPIND1 presented a 2.24-fold change. Strikingly, CD41+ platelets also demonstrated a marked difference of expression in HCC and cirrhosis groups.<h4>Conclusions</h4>Our study reports liquid biopsy-based platelets mRNA signature for early diagnosis of HCC from underlying cirrhotic nodules. Moreover, differential expression of CD41+ platelets in two groups provides new insights into a probable link between CD41 expression on platelets with the progression of cirrhosis to HCC.
format	article
author	Walifa Waqar Sidra Asghar Sobia Manzoor
author_facet	Walifa Waqar Sidra Asghar Sobia Manzoor
author_sort	Walifa Waqar
title	Platelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.
title_short	Platelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.
title_full	Platelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.
title_fullStr	Platelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.
title_full_unstemmed	Platelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.
title_sort	platelets' rna as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7a92a7447fe74c5dbce503d3d2daf94c
work_keys_str_mv	AT walifawaqar plateletsrnaasbiomarkertrovefordifferentiationofearlystagehepatocellularcarcinomafromunderlyingcirrhoticnodules AT sidraasghar plateletsrnaasbiomarkertrovefordifferentiationofearlystagehepatocellularcarcinomafromunderlyingcirrhoticnodules AT sobiamanzoor plateletsrnaasbiomarkertrovefordifferentiationofearlystagehepatocellularcarcinomafromunderlyingcirrhoticnodules
_version_	1718375190811901952

Platelets' RNA as biomarker trove for differentiation of early-stage hepatocellular carcinoma from underlying cirrhotic nodules.

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