Mechanical Regulation of Epithelial Tissue Homeostasis

Despite recent efforts to understand homeostasis in epithelial tissues, there are many unknowns surrounding this steady state. It is considered to be regulated by mechanoresponse, but unlike for single cells, this idea remains heavily debated for tissues. Here, we show that changes in matrix stiffne...

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Autores principales:	Sara Kaliman, Maxime Hubert, Carina Wollnik, Lovro Nuić, Damir Vurnek, Simone Gehrer, Jakov Lovrić, Diana Dudziak, Florian Rehfeldt, Ana-Sunčana Smith
Formato:	article
Lenguaje:	EN
Publicado:	American Physical Society 2021
Materias:	Physics QC1-999
Acceso en línea:	https://doaj.org/article/7a96500a84fc4c628c4545296216ef73
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spelling	oai:doaj.org-article:7a96500a84fc4c628c4545296216ef732021-12-02T16:25:59ZMechanical Regulation of Epithelial Tissue Homeostasis10.1103/PhysRevX.11.0310292160-3308https://doaj.org/article/7a96500a84fc4c628c4545296216ef732021-08-01T00:00:00Zhttp://doi.org/10.1103/PhysRevX.11.031029http://doi.org/10.1103/PhysRevX.11.031029https://doaj.org/toc/2160-3308Despite recent efforts to understand homeostasis in epithelial tissues, there are many unknowns surrounding this steady state. It is considered to be regulated by mechanoresponse, but unlike for single cells, this idea remains heavily debated for tissues. Here, we show that changes in matrix stiffness induce a nonequilibrium transition from tubular to squamous Madin-Darby Canine Kidney II tissues. Nonetheless, despite different cell morphologies and densities, all homeostatic tissues display equivalent topologies, which, hence, must be actively targeted and regulated. On the contrary, the mechanoresponse induces dramatic changes in the large-scale organization of the colonies. On stiff gels, this yields an unreported cooperative state of motile cells displaying higher densities than in the arrested homeostatic state, which suggests a more complex relation between cell density and motility than previously anticipated. Our results unequivocally relate the mechanosensitive properties of individual cells to the evolving macroscopic structures, an effect that could be important for understanding the emergent pathologies of living tissues.Sara KalimanMaxime HubertCarina WollnikLovro NuićDamir VurnekSimone GehrerJakov LovrićDiana DudziakFlorian RehfeldtAna-Sunčana SmithAmerican Physical SocietyarticlePhysicsQC1-999ENPhysical Review X, Vol 11, Iss 3, p 031029 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Physics QC1-999
spellingShingle	Physics QC1-999 Sara Kaliman Maxime Hubert Carina Wollnik Lovro Nuić Damir Vurnek Simone Gehrer Jakov Lovrić Diana Dudziak Florian Rehfeldt Ana-Sunčana Smith Mechanical Regulation of Epithelial Tissue Homeostasis
description	Despite recent efforts to understand homeostasis in epithelial tissues, there are many unknowns surrounding this steady state. It is considered to be regulated by mechanoresponse, but unlike for single cells, this idea remains heavily debated for tissues. Here, we show that changes in matrix stiffness induce a nonequilibrium transition from tubular to squamous Madin-Darby Canine Kidney II tissues. Nonetheless, despite different cell morphologies and densities, all homeostatic tissues display equivalent topologies, which, hence, must be actively targeted and regulated. On the contrary, the mechanoresponse induces dramatic changes in the large-scale organization of the colonies. On stiff gels, this yields an unreported cooperative state of motile cells displaying higher densities than in the arrested homeostatic state, which suggests a more complex relation between cell density and motility than previously anticipated. Our results unequivocally relate the mechanosensitive properties of individual cells to the evolving macroscopic structures, an effect that could be important for understanding the emergent pathologies of living tissues.
format	article
author	Sara Kaliman Maxime Hubert Carina Wollnik Lovro Nuić Damir Vurnek Simone Gehrer Jakov Lovrić Diana Dudziak Florian Rehfeldt Ana-Sunčana Smith
author_facet	Sara Kaliman Maxime Hubert Carina Wollnik Lovro Nuić Damir Vurnek Simone Gehrer Jakov Lovrić Diana Dudziak Florian Rehfeldt Ana-Sunčana Smith
author_sort	Sara Kaliman
title	Mechanical Regulation of Epithelial Tissue Homeostasis
title_short	Mechanical Regulation of Epithelial Tissue Homeostasis
title_full	Mechanical Regulation of Epithelial Tissue Homeostasis
title_fullStr	Mechanical Regulation of Epithelial Tissue Homeostasis
title_full_unstemmed	Mechanical Regulation of Epithelial Tissue Homeostasis
title_sort	mechanical regulation of epithelial tissue homeostasis
publisher	American Physical Society
publishDate	2021
url	https://doaj.org/article/7a96500a84fc4c628c4545296216ef73
work_keys_str_mv	AT sarakaliman mechanicalregulationofepithelialtissuehomeostasis AT maximehubert mechanicalregulationofepithelialtissuehomeostasis AT carinawollnik mechanicalregulationofepithelialtissuehomeostasis AT lovronuic mechanicalregulationofepithelialtissuehomeostasis AT damirvurnek mechanicalregulationofepithelialtissuehomeostasis AT simonegehrer mechanicalregulationofepithelialtissuehomeostasis AT jakovlovric mechanicalregulationofepithelialtissuehomeostasis AT dianadudziak mechanicalregulationofepithelialtissuehomeostasis AT florianrehfeldt mechanicalregulationofepithelialtissuehomeostasis AT anasuncanasmith mechanicalregulationofepithelialtissuehomeostasis
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Mechanical Regulation of Epithelial Tissue Homeostasis

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