Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor

Abstract Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K+) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniqu...

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Autores principales:	Yanjun Ding, Xingmei Li, Yadong Guo, Weicheng Duan, Jiang Ling, Lagabaiyla Zha, Jie Yan, Ying Zou, Jifeng Cai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7a9b2fa0405f40c68230aa5d37702f71
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spelling	oai:doaj.org-article:7a9b2fa0405f40c68230aa5d37702f712021-12-02T11:41:19ZEstimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor10.1038/s41598-017-02027-12045-2322https://doaj.org/article/7a9b2fa0405f40c68230aa5d37702f712017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02027-1https://doaj.org/toc/2045-2322Abstract Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K+) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K+ ions. The linear range for K+ ions was found to be 0.1 nM-1 mM, with limit of detection of 0.06 nM. Moreover, 63 vitreous humour cases within 36 h after death were further studied to verify the utility of K+ ions in estimating the PMI. By the fluorescence aptasensor method, a new formula was built to determine the postmortem interval based on K+ ions concentration: PMI(h) = −0.55 + 1.66 × CK +(r = 0.791). And the real significance of this research was demonstrated by additional 6 cases with known PMIs. In comparison with the conventional method, the presented aptasensor strategy is cost-effective and easy in measuring vitreous K+, which may be potentially a better way for estimation of PMI in medico-legal practice.Yanjun DingXingmei LiYadong GuoWeicheng DuanJiang LingLagabaiyla ZhaJie YanYing ZouJifeng CaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yanjun Ding Xingmei Li Yadong Guo Weicheng Duan Jiang Ling Lagabaiyla Zha Jie Yan Ying Zou Jifeng Cai Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
description	Abstract Estimation of postmortem interval (PMI) is a central role in medico-legal identification. Analysis of vitreous potassium ions (K+) concentration is frequently used by forensic workers to estimate PMI. This paper describes interdisciplinary research to introduce fluorescence sensing techniques into forensic medicine. On the basis of silver nanoclusters (AgNCs) probe stabilized by DNA, a simple and highly sensitive fluorescence aptasensor has been proposed to selectively detect K+ ions. The linear range for K+ ions was found to be 0.1 nM-1 mM, with limit of detection of 0.06 nM. Moreover, 63 vitreous humour cases within 36 h after death were further studied to verify the utility of K+ ions in estimating the PMI. By the fluorescence aptasensor method, a new formula was built to determine the postmortem interval based on K+ ions concentration: PMI(h) = −0.55 + 1.66 × CK +(r = 0.791). And the real significance of this research was demonstrated by additional 6 cases with known PMIs. In comparison with the conventional method, the presented aptasensor strategy is cost-effective and easy in measuring vitreous K+, which may be potentially a better way for estimation of PMI in medico-legal practice.
format	article
author	Yanjun Ding Xingmei Li Yadong Guo Weicheng Duan Jiang Ling Lagabaiyla Zha Jie Yan Ying Zou Jifeng Cai
author_facet	Yanjun Ding Xingmei Li Yadong Guo Weicheng Duan Jiang Ling Lagabaiyla Zha Jie Yan Ying Zou Jifeng Cai
author_sort	Yanjun Ding
title	Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_short	Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_full	Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_fullStr	Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_full_unstemmed	Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
title_sort	estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7a9b2fa0405f40c68230aa5d37702f71
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Estimation of postmortem interval by vitreous potassium evaluation with a novel fluorescence aptasensor

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