Non-invasive single-cell morphometry in living bacterial biofilms

Accurate cell detection in dense bacterial biofilms is challenging. Here, the authors report an image analysis pipeline that is able to accurately segment and classify single bacterial cells in 3D fluorescence images: Bacterial Cell Morphometry 3D (BCM3D).

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Autores principales: Mingxing Zhang, Ji Zhang, Yibo Wang, Jie Wang, Alecia M. Achimovich, Scott T. Acton, Andreas Gahlmann
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/7a9bc6ded39a44f8ba843f3cc5a84278
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spelling oai:doaj.org-article:7a9bc6ded39a44f8ba843f3cc5a842782021-12-02T15:39:07ZNon-invasive single-cell morphometry in living bacterial biofilms10.1038/s41467-020-19866-82041-1723https://doaj.org/article/7a9bc6ded39a44f8ba843f3cc5a842782020-12-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19866-8https://doaj.org/toc/2041-1723Accurate cell detection in dense bacterial biofilms is challenging. Here, the authors report an image analysis pipeline that is able to accurately segment and classify single bacterial cells in 3D fluorescence images: Bacterial Cell Morphometry 3D (BCM3D).Mingxing ZhangJi ZhangYibo WangJie WangAlecia M. AchimovichScott T. ActonAndreas GahlmannNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Mingxing Zhang
Ji Zhang
Yibo Wang
Jie Wang
Alecia M. Achimovich
Scott T. Acton
Andreas Gahlmann
Non-invasive single-cell morphometry in living bacterial biofilms
description Accurate cell detection in dense bacterial biofilms is challenging. Here, the authors report an image analysis pipeline that is able to accurately segment and classify single bacterial cells in 3D fluorescence images: Bacterial Cell Morphometry 3D (BCM3D).
format article
author Mingxing Zhang
Ji Zhang
Yibo Wang
Jie Wang
Alecia M. Achimovich
Scott T. Acton
Andreas Gahlmann
author_facet Mingxing Zhang
Ji Zhang
Yibo Wang
Jie Wang
Alecia M. Achimovich
Scott T. Acton
Andreas Gahlmann
author_sort Mingxing Zhang
title Non-invasive single-cell morphometry in living bacterial biofilms
title_short Non-invasive single-cell morphometry in living bacterial biofilms
title_full Non-invasive single-cell morphometry in living bacterial biofilms
title_fullStr Non-invasive single-cell morphometry in living bacterial biofilms
title_full_unstemmed Non-invasive single-cell morphometry in living bacterial biofilms
title_sort non-invasive single-cell morphometry in living bacterial biofilms
publisher Nature Portfolio
publishDate 2020
url https://doaj.org/article/7a9bc6ded39a44f8ba843f3cc5a84278
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AT jizhang noninvasivesinglecellmorphometryinlivingbacterialbiofilms
AT yibowang noninvasivesinglecellmorphometryinlivingbacterialbiofilms
AT jiewang noninvasivesinglecellmorphometryinlivingbacterialbiofilms
AT aleciamachimovich noninvasivesinglecellmorphometryinlivingbacterialbiofilms
AT scotttacton noninvasivesinglecellmorphometryinlivingbacterialbiofilms
AT andreasgahlmann noninvasivesinglecellmorphometryinlivingbacterialbiofilms
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