Mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers

We studied capabilities of confocal laser scanning microscopy in the analysis of lipid droplets volume and of quantity of functional mitochondria and reactive oxygen species production in liver cells for early diagnosis of cytochemical disturbances at dyslipoproteinemia (16 days of experiment). The...

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Autores principales:	N. P. Sudakov, I. V. Klimenkov, A. I. Katyshev, S. B. Nikiforov, O. A. Goldberg, B. G. Pushkaryov, S. A. Lepekhova, K. A. Apartsin, Y. M. Konstantinov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2016
Materias:	dyslipoproteinemia atherosclerosis myocardial infarction mitochondria reactive oxygen species lipid droplets mitochondrial dna danger associated molecular patterns Science Q
Acceso en línea:	https://doaj.org/article/7aa8cfd2507e4be88465936d8ca61eb5
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spelling	oai:doaj.org-article:7aa8cfd2507e4be88465936d8ca61eb52021-11-23T06:14:34ZMitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers2541-94202587-959610.12737/article_590823a517c941.46556762https://doaj.org/article/7aa8cfd2507e4be88465936d8ca61eb52016-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/205https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596We studied capabilities of confocal laser scanning microscopy in the analysis of lipid droplets volume and of quantity of functional mitochondria and reactive oxygen species production in liver cells for early diagnosis of cytochemical disturbances at dyslipoproteinemia (16 days of experiment). The results showed the increase of lipid droplets volume in hepatocytes, decrease of functional mitochondria and increase of reactive oxygen species production. We evaluated the potential of real-time PCR method in the analysis of mitochondrial DNA of blood plasma at early stages of dyslipoproteinemia and in experimental infarction. On the background of registered blood lipid metabolism disorders and structural and functional changes in liver cells, we determined the tendency to three-time increase in concentration of circulating cell-free mtDNA on the 16th day of dyslipoproteinemia as compared to the control data. We used a model of myocardial infarction to show statistically significant increase in the level of circulating cell-free blood mtDNA from 48 hours after adrenaline injection and we found that this level maintained up to 144 hours after adrenaline injection. Obtained data can serve as a basis for creation of technologies for diagnostic monitoring of atherosclerosis and myocardial infarction severity.N. P. SudakovI. V. KlimenkovA. I. KatyshevS. B. NikiforovO. A. GoldbergB. G. PushkaryovS. A. LepekhovaK. A. ApartsinY. M. KonstantinovScientific Сentre for Family Health and Human Reproduction Problemsarticledyslipoproteinemiaatherosclerosismyocardial infarctionmitochondriareactive oxygen specieslipid dropletsmitochondrial dnadanger associated molecular patternsScienceQRUActa Biomedica Scientifica, Vol 1, Iss 3(2), Pp 131-134 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	dyslipoproteinemia atherosclerosis myocardial infarction mitochondria reactive oxygen species lipid droplets mitochondrial dna danger associated molecular patterns Science Q
spellingShingle	dyslipoproteinemia atherosclerosis myocardial infarction mitochondria reactive oxygen species lipid droplets mitochondrial dna danger associated molecular patterns Science Q N. P. Sudakov I. V. Klimenkov A. I. Katyshev S. B. Nikiforov O. A. Goldberg B. G. Pushkaryov S. A. Lepekhova K. A. Apartsin Y. M. Konstantinov Mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers
description	We studied capabilities of confocal laser scanning microscopy in the analysis of lipid droplets volume and of quantity of functional mitochondria and reactive oxygen species production in liver cells for early diagnosis of cytochemical disturbances at dyslipoproteinemia (16 days of experiment). The results showed the increase of lipid droplets volume in hepatocytes, decrease of functional mitochondria and increase of reactive oxygen species production. We evaluated the potential of real-time PCR method in the analysis of mitochondrial DNA of blood plasma at early stages of dyslipoproteinemia and in experimental infarction. On the background of registered blood lipid metabolism disorders and structural and functional changes in liver cells, we determined the tendency to three-time increase in concentration of circulating cell-free mtDNA on the 16th day of dyslipoproteinemia as compared to the control data. We used a model of myocardial infarction to show statistically significant increase in the level of circulating cell-free blood mtDNA from 48 hours after adrenaline injection and we found that this level maintained up to 144 hours after adrenaline injection. Obtained data can serve as a basis for creation of technologies for diagnostic monitoring of atherosclerosis and myocardial infarction severity.
format	article
author	N. P. Sudakov I. V. Klimenkov A. I. Katyshev S. B. Nikiforov O. A. Goldberg B. G. Pushkaryov S. A. Lepekhova K. A. Apartsin Y. M. Konstantinov
author_facet	N. P. Sudakov I. V. Klimenkov A. I. Katyshev S. B. Nikiforov O. A. Goldberg B. G. Pushkaryov S. A. Lepekhova K. A. Apartsin Y. M. Konstantinov
author_sort	N. P. Sudakov
title	Mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers
title_short	Mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers
title_full	Mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers
title_fullStr	Mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers
title_full_unstemmed	Mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers
title_sort	mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2016
url	https://doaj.org/article/7aa8cfd2507e4be88465936d8ca61eb5
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Mitochondrial dysfunction at atherosclerosis and myocardial infarction: molecular and cytochemical cell-markers

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