Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.

The cilium, the sensing centre for the cell, displays an extensive repertoire of receptors for various cell signalling processes. The dynamic nature of ciliary signalling indicates that the ciliary entry of receptors and associated proteins must be regulated and conditional. To understand this proce...

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Autores principales:	Swadhin Chandra Jana, Priya Dutta, Akanksha Jain, Anjusha Singh, Lavanya Adusumilli, Mukul Girotra, Diksha Kumari, Seema Shirolikar, Krishanu Ray
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/7aa8f5ee7acb403eb8c75b91223fc765
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spelling	oai:doaj.org-article:7aa8f5ee7acb403eb8c75b91223fc7652021-12-02T20:02:52ZKinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.1553-73901553-740410.1371/journal.pgen.1009752https://doaj.org/article/7aa8f5ee7acb403eb8c75b91223fc7652021-08-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009752https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404The cilium, the sensing centre for the cell, displays an extensive repertoire of receptors for various cell signalling processes. The dynamic nature of ciliary signalling indicates that the ciliary entry of receptors and associated proteins must be regulated and conditional. To understand this process, we studied the ciliary localisation of the odour-receptor coreceptor (Orco), a seven-pass transmembrane protein essential for insect olfaction. Little is known about when and how Orco gets into the cilia. Here, using Drosophila melanogaster, we show that the bulk of Orco selectively enters the cilia on adult olfactory sensory neurons in two discrete, one-hour intervals after eclosion. A conditional loss of heterotrimeric kinesin-2 during this period reduces the electrophysiological response to odours and affects olfactory behaviour. We further show that Orco binds to the C-terminal tail fragments of the heterotrimeric kinesin-2 motor, which is required to transfer Orco from the ciliary base to the outer segment and maintain within an approximately four-micron stretch at the distal portion of the ciliary outer-segment. The Orco transport was not affected by the loss of critical intraflagellar transport components, IFT172/Oseg2 and IFT88/NompB, respectively, during the adult stage. These results highlight a novel developmental regulation of seven-pass transmembrane receptor transport into the cilia and indicate that ciliary signalling is both developmentally and temporally regulated.Swadhin Chandra JanaPriya DuttaAkanksha JainAnjusha SinghLavanya AdusumilliMukul GirotraDiksha KumariSeema ShirolikarKrishanu RayPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 8, p e1009752 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Swadhin Chandra Jana Priya Dutta Akanksha Jain Anjusha Singh Lavanya Adusumilli Mukul Girotra Diksha Kumari Seema Shirolikar Krishanu Ray Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.
description	The cilium, the sensing centre for the cell, displays an extensive repertoire of receptors for various cell signalling processes. The dynamic nature of ciliary signalling indicates that the ciliary entry of receptors and associated proteins must be regulated and conditional. To understand this process, we studied the ciliary localisation of the odour-receptor coreceptor (Orco), a seven-pass transmembrane protein essential for insect olfaction. Little is known about when and how Orco gets into the cilia. Here, using Drosophila melanogaster, we show that the bulk of Orco selectively enters the cilia on adult olfactory sensory neurons in two discrete, one-hour intervals after eclosion. A conditional loss of heterotrimeric kinesin-2 during this period reduces the electrophysiological response to odours and affects olfactory behaviour. We further show that Orco binds to the C-terminal tail fragments of the heterotrimeric kinesin-2 motor, which is required to transfer Orco from the ciliary base to the outer segment and maintain within an approximately four-micron stretch at the distal portion of the ciliary outer-segment. The Orco transport was not affected by the loss of critical intraflagellar transport components, IFT172/Oseg2 and IFT88/NompB, respectively, during the adult stage. These results highlight a novel developmental regulation of seven-pass transmembrane receptor transport into the cilia and indicate that ciliary signalling is both developmentally and temporally regulated.
format	article
author	Swadhin Chandra Jana Priya Dutta Akanksha Jain Anjusha Singh Lavanya Adusumilli Mukul Girotra Diksha Kumari Seema Shirolikar Krishanu Ray
author_facet	Swadhin Chandra Jana Priya Dutta Akanksha Jain Anjusha Singh Lavanya Adusumilli Mukul Girotra Diksha Kumari Seema Shirolikar Krishanu Ray
author_sort	Swadhin Chandra Jana
title	Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.
title_short	Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.
title_full	Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.
title_fullStr	Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.
title_full_unstemmed	Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.
title_sort	kinesin-2 transports orco into the olfactory cilium of drosophila melanogaster at specific developmental stages.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/7aa8f5ee7acb403eb8c75b91223fc765
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Kinesin-2 transports Orco into the olfactory cilium of Drosophila melanogaster at specific developmental stages.

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