In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons

Abstract Altered dendritic morphology is common in neurodevelopmental disorders (NDDs), many of which show sex biases in prevalence, onset and/or severity. However, whether dendritic morphology varies as a function of sex in juvenile mice or primary neuronal cell cultures is largely unknown even tho...

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Autores principales:	Kimberly P. Keil, Sunjay Sethi, Machelle D. Wilson, Hao Chen, Pamela J. Lein
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ab63f07b5054d8da52e7b5b426f7176
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spelling	oai:doaj.org-article:7ab63f07b5054d8da52e7b5b426f71762021-12-02T16:08:23ZIn vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons10.1038/s41598-017-08459-z2045-2322https://doaj.org/article/7ab63f07b5054d8da52e7b5b426f71762017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08459-zhttps://doaj.org/toc/2045-2322Abstract Altered dendritic morphology is common in neurodevelopmental disorders (NDDs), many of which show sex biases in prevalence, onset and/or severity. However, whether dendritic morphology varies as a function of sex in juvenile mice or primary neuronal cell cultures is largely unknown even though both are widely used models for studying NDDs. To address this gap, we quantified dendritic morphology in CA1 pyramidal hippocampal and adjacent somatosensory pyramidal cortical neurons from male and female postnatal day (P)28 C57BL/6J mice. As determined by Sholl analysis of Golgi-stained brain sections, dendritic arbors of male hippocampal neurons are more complex than females. Conversely, dendritic morphology of female cortical neurons is more complex than males. In primary neuron-glia co-cultures from P0 mouse hippocampi, male neurons have more complex dendritic arbors than female neurons. Sex differences are less pronounced in cortical cultures. In vitro sex differences in dendritic morphology are driven in part by estrogen-dependent mechanisms, as evidenced by decreased dendritic complexity in male hippocampal neurons cultured in phenol red-free media or in the presence of an estrogen receptor antagonist. Evidence that sex influences dendritic morphogenesis in two models of neurodevelopment in a region-specific manner has significant mechanistic implications regarding sex biases in NDDs.Kimberly P. KeilSunjay SethiMachelle D. WilsonHao ChenPamela J. LeinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kimberly P. Keil Sunjay Sethi Machelle D. Wilson Hao Chen Pamela J. Lein In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons
description	Abstract Altered dendritic morphology is common in neurodevelopmental disorders (NDDs), many of which show sex biases in prevalence, onset and/or severity. However, whether dendritic morphology varies as a function of sex in juvenile mice or primary neuronal cell cultures is largely unknown even though both are widely used models for studying NDDs. To address this gap, we quantified dendritic morphology in CA1 pyramidal hippocampal and adjacent somatosensory pyramidal cortical neurons from male and female postnatal day (P)28 C57BL/6J mice. As determined by Sholl analysis of Golgi-stained brain sections, dendritic arbors of male hippocampal neurons are more complex than females. Conversely, dendritic morphology of female cortical neurons is more complex than males. In primary neuron-glia co-cultures from P0 mouse hippocampi, male neurons have more complex dendritic arbors than female neurons. Sex differences are less pronounced in cortical cultures. In vitro sex differences in dendritic morphology are driven in part by estrogen-dependent mechanisms, as evidenced by decreased dendritic complexity in male hippocampal neurons cultured in phenol red-free media or in the presence of an estrogen receptor antagonist. Evidence that sex influences dendritic morphogenesis in two models of neurodevelopment in a region-specific manner has significant mechanistic implications regarding sex biases in NDDs.
format	article
author	Kimberly P. Keil Sunjay Sethi Machelle D. Wilson Hao Chen Pamela J. Lein
author_facet	Kimberly P. Keil Sunjay Sethi Machelle D. Wilson Hao Chen Pamela J. Lein
author_sort	Kimberly P. Keil
title	In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons
title_short	In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons
title_full	In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons
title_fullStr	In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons
title_full_unstemmed	In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons
title_sort	in vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7ab63f07b5054d8da52e7b5b426f7176
work_keys_str_mv	AT kimberlypkeil invivoandinvitrosexdifferencesinthedendriticmorphologyofdevelopingmurinehippocampalandcorticalneurons AT sunjaysethi invivoandinvitrosexdifferencesinthedendriticmorphologyofdevelopingmurinehippocampalandcorticalneurons AT machelledwilson invivoandinvitrosexdifferencesinthedendriticmorphologyofdevelopingmurinehippocampalandcorticalneurons AT haochen invivoandinvitrosexdifferencesinthedendriticmorphologyofdevelopingmurinehippocampalandcorticalneurons AT pamelajlein invivoandinvitrosexdifferencesinthedendriticmorphologyofdevelopingmurinehippocampalandcorticalneurons
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In vivo and in vitro sex differences in the dendritic morphology of developing murine hippocampal and cortical neurons

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