Analysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations

Background: Specific diagnostic markers for congenital pulmonary airway malformations (CPAMs) have not yet been discovered. This study intends to detect differentially expressed miRNAs in type I and type II CPAMs by using a miRNA chip and clarify the feasibility of miRNAs as different CPAM typing ma...

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Autores principales:	Jiahang Zeng, Wei Liu, Jianhua Liang, Junzheng Peng, Fenghua Wang, Jue Tang, Qinglin Yang, Linwei Zhuang, Dongmei Huang, Le Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	miRNA congenital pulmonary airway malformations biomarker miRNA chip type I and type II CPAM Pediatrics RJ1-570
Acceso en línea:	https://doaj.org/article/7ab9061b0b5c4672921bff0769e54112
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spelling	oai:doaj.org-article:7ab9061b0b5c4672921bff0769e541122021-11-18T09:50:59ZAnalysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations2296-236010.3389/fped.2021.671107https://doaj.org/article/7ab9061b0b5c4672921bff0769e541122021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fped.2021.671107/fullhttps://doaj.org/toc/2296-2360Background: Specific diagnostic markers for congenital pulmonary airway malformations (CPAMs) have not yet been discovered. This study intends to detect differentially expressed miRNAs in type I and type II CPAMs by using a miRNA chip and clarify the feasibility of miRNAs as different CPAM typing markers.Methods: Lung tissues of type I and type II CPAMs were collected and used to assess the differentially expressed miRNAs using a miRNA chip after evaluation using hematoxylin–eosin staining and Masson staining. Quantitative reverse transcription-polymerase chain reaction and fluorescence in situ hybridization were used to verify the quality of the miRNA chip. The function and pathways of related differentially expressed miRNAs were analyzed by Gene Ontology Enrichment (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, respectively. Targets of miRNAs were predicted by targetscan7.1 and mirdbV6 and the network between miRNA and mRNA was established using Cystoscope software.Results: In total, 394/34 upregulated and 321/72 downregulated miRNAs were found in type I and type II CPAMs, respectively. GO and KEGG analysis showed that different pathways are involved in the regulation of CPAM, including platelet activation, Ras, MAPK, FoxO, and PI3K-Akt signaling pathways. miRNA–mRNA network analysis confirmed four major miRNAs in CPAM, including miR-4731-5p to complexin 2, miR-3150a-3p to vesicle amine transport 1, miR-32-5p to F-box and WD repeat domain containing 7, and miR-454-3p to SLAIN motif family member 1.Conclusion: In summary, we have identified four candidate miRNAs and pathways related to different pattern CPAMs, which provide a new perspective for CPAM research and treatment.Jiahang ZengWei LiuJianhua LiangJunzheng PengFenghua WangJue TangQinglin YangLinwei ZhuangDongmei HuangLe LiFrontiers Media S.A.articlemiRNAcongenital pulmonary airway malformationsbiomarkermiRNA chiptype I and type II CPAMPediatricsRJ1-570ENFrontiers in Pediatrics, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	miRNA congenital pulmonary airway malformations biomarker miRNA chip type I and type II CPAM Pediatrics RJ1-570
spellingShingle	miRNA congenital pulmonary airway malformations biomarker miRNA chip type I and type II CPAM Pediatrics RJ1-570 Jiahang Zeng Wei Liu Jianhua Liang Junzheng Peng Fenghua Wang Jue Tang Qinglin Yang Linwei Zhuang Dongmei Huang Le Li Analysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations
description	Background: Specific diagnostic markers for congenital pulmonary airway malformations (CPAMs) have not yet been discovered. This study intends to detect differentially expressed miRNAs in type I and type II CPAMs by using a miRNA chip and clarify the feasibility of miRNAs as different CPAM typing markers.Methods: Lung tissues of type I and type II CPAMs were collected and used to assess the differentially expressed miRNAs using a miRNA chip after evaluation using hematoxylin–eosin staining and Masson staining. Quantitative reverse transcription-polymerase chain reaction and fluorescence in situ hybridization were used to verify the quality of the miRNA chip. The function and pathways of related differentially expressed miRNAs were analyzed by Gene Ontology Enrichment (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, respectively. Targets of miRNAs were predicted by targetscan7.1 and mirdbV6 and the network between miRNA and mRNA was established using Cystoscope software.Results: In total, 394/34 upregulated and 321/72 downregulated miRNAs were found in type I and type II CPAMs, respectively. GO and KEGG analysis showed that different pathways are involved in the regulation of CPAM, including platelet activation, Ras, MAPK, FoxO, and PI3K-Akt signaling pathways. miRNA–mRNA network analysis confirmed four major miRNAs in CPAM, including miR-4731-5p to complexin 2, miR-3150a-3p to vesicle amine transport 1, miR-32-5p to F-box and WD repeat domain containing 7, and miR-454-3p to SLAIN motif family member 1.Conclusion: In summary, we have identified four candidate miRNAs and pathways related to different pattern CPAMs, which provide a new perspective for CPAM research and treatment.
format	article
author	Jiahang Zeng Wei Liu Jianhua Liang Junzheng Peng Fenghua Wang Jue Tang Qinglin Yang Linwei Zhuang Dongmei Huang Le Li
author_facet	Jiahang Zeng Wei Liu Jianhua Liang Junzheng Peng Fenghua Wang Jue Tang Qinglin Yang Linwei Zhuang Dongmei Huang Le Li
author_sort	Jiahang Zeng
title	Analysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations
title_short	Analysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations
title_full	Analysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations
title_fullStr	Analysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations
title_full_unstemmed	Analysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations
title_sort	analysis of mirna profiles and the regulatory network in congenital pulmonary airway malformations
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/7ab9061b0b5c4672921bff0769e54112
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_version_	1718420853958377472

Analysis of miRNA Profiles and the Regulatory Network in Congenital Pulmonary Airway Malformations

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