Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity

Arek M Engstrom,1 Ryan A Faase,2 Grant W Marquart,3 Joe E Baio,2 Marilyn R Mackiewicz,3 Stacey L Harper1,2,4 1Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States; 2School of Chemical, Biological, and Environmental Engineering, Oregon State Univ...

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Autores principales:	Engstrom AM, Faase RA, Marquart GW, Baio JE, Mackiewicz MR, Harper SL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	gold nanoparticle vibrational spectroscopy nanoparticle-biological interactions hybrid-lipid coated nanoparticle toxicity size-dependent interaction zebrafish Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7ac630b169a343cb9571a232baf69519
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spelling	oai:doaj.org-article:7ac630b169a343cb9571a232baf695192021-12-02T10:58:58ZSize-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity1178-2013https://doaj.org/article/7ac630b169a343cb9571a232baf695192020-06-01T00:00:00Zhttps://www.dovepress.com/size-dependent-interactions-of-lipid-coated-gold-nanoparticles-develop-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Arek M Engstrom,1 Ryan A Faase,2 Grant W Marquart,3 Joe E Baio,2 Marilyn R Mackiewicz,3 Stacey L Harper1,2,4 1Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States; 2School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR, United States; 3Department of Chemistry, Portland State University, Portland, OR, United States; 4Oregon Nanoscience and Microtechnologies Institute, Corvallis, OR, United StatesCorrespondence: Stacey L Harper Email Stacey.Harper@oregonstate.eduIntroduction: Humans are intentionally exposed to gold nanoparticles (AuNPs) where they are used in variety of biomedical applications as imaging and drug delivery agents as well as diagnostic and therapeutic agents currently in clinic and in a variety of upcoming clinical trials. Consequently, it is critical that we gain a better understanding of how physiochemical properties such as size, shape, and surface chemistry drive cellular uptake and AuNP toxicity in vivo. Understanding and being able to manipulate these physiochemical properties will allow for the production of safer and more efficacious use of AuNPs in biomedical applications.Methods and Materials: Here, AuNPs of three sizes, 5 nm, 10 nm, and 20 nm, were coated with a lipid bilayer composed of sodium oleate, hydrogenated phosphatidylcholine, and hexanethiol. To understand how the physical features of AuNPs influence uptake through cellular membranes, sum frequency generation (SFG) was utilized to assess the interactions of the AuNPs with a biomimetic lipid monolayer composed of a deuterated phospholipid 1.2-dipalmitoyl-d62-sn-glycero-3-phosphocholine (dDPPC).Results and Discussion: SFG measurements showed that 5 nm and 10 nm AuNPs are able to phase into the lipid monolayer with very little energetic cost, whereas, the 20 nm AuNPs warped the membrane conforming it to the curvature of hybrid lipid-coated AuNPs. Toxicity of the AuNPs were assessed in vivo to determine how AuNP curvature and uptake influence cell health. In contrast, in vivo toxicity tested in embryonic zebrafish showed rapid toxicity of the 5 nm AuNPs, with significant 24 hpf mortality occurring at concentrations ≥ 20 mg/L, whereas the 10 nm and 20 nm AuNPs showed no significant mortality throughout the five-day experiment.Conclusion: By combining information from membrane models using SFG spectroscopy with in vivo toxicity studies, a better mechanistic understanding of how nanoparticles (NPs) interact with membranes is developed to understand how the physiochemical features of AuNPs drive nanoparticle–membrane interactions, cellular uptake, and toxicity.Keywords: gold nanoparticle, vibrational spectroscopy, nanoparticle–biological interactions, hybrid lipid-coated nanoparticle, toxicity, size-dependent interaction, zebrafishEngstrom AMFaase RAMarquart GWBaio JEMackiewicz MRHarper SLDove Medical Pressarticlegold nanoparticlevibrational spectroscopynanoparticle-biological interactionshybrid-lipid coated nanoparticletoxicitysize-dependent interactionzebrafishMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 4091-4104 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	gold nanoparticle vibrational spectroscopy nanoparticle-biological interactions hybrid-lipid coated nanoparticle toxicity size-dependent interaction zebrafish Medicine (General) R5-920
spellingShingle	gold nanoparticle vibrational spectroscopy nanoparticle-biological interactions hybrid-lipid coated nanoparticle toxicity size-dependent interaction zebrafish Medicine (General) R5-920 Engstrom AM Faase RA Marquart GW Baio JE Mackiewicz MR Harper SL Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity
description	Arek M Engstrom,1 Ryan A Faase,2 Grant W Marquart,3 Joe E Baio,2 Marilyn R Mackiewicz,3 Stacey L Harper1,2,4 1Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, United States; 2School of Chemical, Biological, and Environmental Engineering, Oregon State University, Corvallis, OR, United States; 3Department of Chemistry, Portland State University, Portland, OR, United States; 4Oregon Nanoscience and Microtechnologies Institute, Corvallis, OR, United StatesCorrespondence: Stacey L Harper Email Stacey.Harper@oregonstate.eduIntroduction: Humans are intentionally exposed to gold nanoparticles (AuNPs) where they are used in variety of biomedical applications as imaging and drug delivery agents as well as diagnostic and therapeutic agents currently in clinic and in a variety of upcoming clinical trials. Consequently, it is critical that we gain a better understanding of how physiochemical properties such as size, shape, and surface chemistry drive cellular uptake and AuNP toxicity in vivo. Understanding and being able to manipulate these physiochemical properties will allow for the production of safer and more efficacious use of AuNPs in biomedical applications.Methods and Materials: Here, AuNPs of three sizes, 5 nm, 10 nm, and 20 nm, were coated with a lipid bilayer composed of sodium oleate, hydrogenated phosphatidylcholine, and hexanethiol. To understand how the physical features of AuNPs influence uptake through cellular membranes, sum frequency generation (SFG) was utilized to assess the interactions of the AuNPs with a biomimetic lipid monolayer composed of a deuterated phospholipid 1.2-dipalmitoyl-d62-sn-glycero-3-phosphocholine (dDPPC).Results and Discussion: SFG measurements showed that 5 nm and 10 nm AuNPs are able to phase into the lipid monolayer with very little energetic cost, whereas, the 20 nm AuNPs warped the membrane conforming it to the curvature of hybrid lipid-coated AuNPs. Toxicity of the AuNPs were assessed in vivo to determine how AuNP curvature and uptake influence cell health. In contrast, in vivo toxicity tested in embryonic zebrafish showed rapid toxicity of the 5 nm AuNPs, with significant 24 hpf mortality occurring at concentrations ≥ 20 mg/L, whereas the 10 nm and 20 nm AuNPs showed no significant mortality throughout the five-day experiment.Conclusion: By combining information from membrane models using SFG spectroscopy with in vivo toxicity studies, a better mechanistic understanding of how nanoparticles (NPs) interact with membranes is developed to understand how the physiochemical features of AuNPs drive nanoparticle–membrane interactions, cellular uptake, and toxicity.Keywords: gold nanoparticle, vibrational spectroscopy, nanoparticle–biological interactions, hybrid lipid-coated nanoparticle, toxicity, size-dependent interaction, zebrafish
format	article
author	Engstrom AM Faase RA Marquart GW Baio JE Mackiewicz MR Harper SL
author_facet	Engstrom AM Faase RA Marquart GW Baio JE Mackiewicz MR Harper SL
author_sort	Engstrom AM
title	Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity
title_short	Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity
title_full	Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity
title_fullStr	Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity
title_full_unstemmed	Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity
title_sort	size-dependent interactions of lipid-coated gold nanoparticles: developing a better mechanistic understanding through model cell membranes and in vivo toxicity
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/7ac630b169a343cb9571a232baf69519
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_version_	1718396407496310784

Size-Dependent Interactions of Lipid-Coated Gold Nanoparticles: Developing a Better Mechanistic Understanding Through Model Cell Membranes and in vivo Toxicity

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