Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii

Abstract In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenoty...

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Autores principales:	Deja Rodgers, Casin Le, Camila Pimentel, Marisel R. Tuttobene, Tomás Subils, Jenny Escalante, Brent Nishimura, Eleonora García Vescovi, Rodrigo Sieira, Robert A. Bonomo, Marcelo E. Tolmasky, Maria Soledad Ramirez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7acb0c0de2d34ba8a9d953eb794b2659
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spelling	oai:doaj.org-article:7acb0c0de2d34ba8a9d953eb794b26592021-12-02T18:33:47ZHistone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii10.1038/s41598-021-98101-w2045-2322https://doaj.org/article/7acb0c0de2d34ba8a9d953eb794b26592021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98101-whttps://doaj.org/toc/2045-2322Abstract In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an altered stress response. To further contribute to the knowledge of this key transcriptional regulator in A. baumannii behavior, we studied the role of H-NS in antimicrobial resistance. Using two well characterized A. baumannii model strains with distinctive resistance profile and pathogenicity traits (AB5075 and A118), complementary transcriptomic and phenotypic approaches were used to study the role of H-NS in antimicrobial resistance, biofilm and quorum sensing gene expression. An increased expression of genes associated with β-lactam resistance, aminoglycosides, quinolones, chloramphenicol, trimethoprim and sulfonamides resistance in the Δhns mutant background was observed. Genes codifying for efflux pumps were also up-regulated, with the exception of adeFGH. The wild-type transcriptional level was restored in the complemented strain. In addition, the expression of biofilm related genes and biofilm production was lowered when the transcriptional repressor was absent. The quorum network genes aidA, abaI, kar and fadD were up-regulated in Δhns mutant strains. Overall, our results showed the complexity and scope of the regulatory network control by H-NS (genes involved in antibiotic resistance and persistence). These observations brings us one step closer to understanding the regulatory role of hns to combat A. baumannii infections.Deja RodgersCasin LeCamila PimentelMarisel R. TuttobeneTomás SubilsJenny EscalanteBrent NishimuraEleonora García VescoviRodrigo SieiraRobert A. BonomoMarcelo E. TolmaskyMaria Soledad RamirezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Deja Rodgers Casin Le Camila Pimentel Marisel R. Tuttobene Tomás Subils Jenny Escalante Brent Nishimura Eleonora García Vescovi Rodrigo Sieira Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
description	Abstract In the multidrug resistant (MDR) pathogen Acinetobacter baumannii the global repressor H-NS was shown to modulate the expression of genes involved in pathogenesis and stress response. In addition, H-NS inactivation results in an increased resistance to colistin, and in a hypermotile phenotype an altered stress response. To further contribute to the knowledge of this key transcriptional regulator in A. baumannii behavior, we studied the role of H-NS in antimicrobial resistance. Using two well characterized A. baumannii model strains with distinctive resistance profile and pathogenicity traits (AB5075 and A118), complementary transcriptomic and phenotypic approaches were used to study the role of H-NS in antimicrobial resistance, biofilm and quorum sensing gene expression. An increased expression of genes associated with β-lactam resistance, aminoglycosides, quinolones, chloramphenicol, trimethoprim and sulfonamides resistance in the Δhns mutant background was observed. Genes codifying for efflux pumps were also up-regulated, with the exception of adeFGH. The wild-type transcriptional level was restored in the complemented strain. In addition, the expression of biofilm related genes and biofilm production was lowered when the transcriptional repressor was absent. The quorum network genes aidA, abaI, kar and fadD were up-regulated in Δhns mutant strains. Overall, our results showed the complexity and scope of the regulatory network control by H-NS (genes involved in antibiotic resistance and persistence). These observations brings us one step closer to understanding the regulatory role of hns to combat A. baumannii infections.
format	article
author	Deja Rodgers Casin Le Camila Pimentel Marisel R. Tuttobene Tomás Subils Jenny Escalante Brent Nishimura Eleonora García Vescovi Rodrigo Sieira Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez
author_facet	Deja Rodgers Casin Le Camila Pimentel Marisel R. Tuttobene Tomás Subils Jenny Escalante Brent Nishimura Eleonora García Vescovi Rodrigo Sieira Robert A. Bonomo Marcelo E. Tolmasky Maria Soledad Ramirez
author_sort	Deja Rodgers
title	Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_short	Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_full	Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_fullStr	Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_full_unstemmed	Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii
title_sort	histone-like nucleoid-structuring protein (h-ns) regulatory role in antibiotic resistance in acinetobacter baumannii
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7acb0c0de2d34ba8a9d953eb794b2659
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Histone-like nucleoid-structuring protein (H-NS) regulatory role in antibiotic resistance in Acinetobacter baumannii

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