Silencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.

Alpha-fetoprotein not only serves as a diagnostic marker for liver cancer, but also posses a variety of biological functions. However, the role of Alpha-fetoprotein on tumor angiogenesis and cell invasion remains incompletely understood. In this study, we aimed to evaluate if Alpha-fetoprotein can r...

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Autores principales:	Wenbo Meng, Xun Li, Zhongtian Bai, Yan Li, Jinqiu Yuan, Tao Liu, Jun Yan, Wence Zhou, Kexiang Zhu, Hui Zhang, Yumin Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7accb7d77ea44239b687b2ea9e242ddf
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spelling	oai:doaj.org-article:7accb7d77ea44239b687b2ea9e242ddf2021-11-18T08:30:13ZSilencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.1932-620310.1371/journal.pone.0090660https://doaj.org/article/7accb7d77ea44239b687b2ea9e242ddf2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24587407/?tool=EBIhttps://doaj.org/toc/1932-6203Alpha-fetoprotein not only serves as a diagnostic marker for liver cancer, but also posses a variety of biological functions. However, the role of Alpha-fetoprotein on tumor angiogenesis and cell invasion remains incompletely understood. In this study, we aimed to evaluate if Alpha-fetoprotein can regulate the major angiogenic factors and matrix metalloproteinases in human liver cancer cells. Alpha-fetoprotein silencing was achieved by Stealth RNAi. Expression of Alpha-fetoprotein was examined by a full-automatic electrochemistry luminescence immunity analyzer. Expression of VEGF, VEGFR-2, MMP-9, and MMP-2 was examined by Western blot and immunocytochemistry. Apoptosis was detected by TUNEL assay. Angiogenesis was detected by in vitro angiogenesis assay kit. Silencing of Alpha-fetoprotein led to an increased apoptosis, which was associated with a decreased expression of vascular endothelial growth factor, vascular endothelial growth factor receptor 2, matrix metalloproteinases-2/9. These results suggest that Alpha-fetoprotein may play a regulatory role on angiogenesis and cell invasion during liver cancer development.Wenbo MengXun LiZhongtian BaiYan LiJinqiu YuanTao LiuJun YanWence ZhouKexiang ZhuHui ZhangYumin LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e90660 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenbo Meng Xun Li Zhongtian Bai Yan Li Jinqiu Yuan Tao Liu Jun Yan Wence Zhou Kexiang Zhu Hui Zhang Yumin Li Silencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.
description	Alpha-fetoprotein not only serves as a diagnostic marker for liver cancer, but also posses a variety of biological functions. However, the role of Alpha-fetoprotein on tumor angiogenesis and cell invasion remains incompletely understood. In this study, we aimed to evaluate if Alpha-fetoprotein can regulate the major angiogenic factors and matrix metalloproteinases in human liver cancer cells. Alpha-fetoprotein silencing was achieved by Stealth RNAi. Expression of Alpha-fetoprotein was examined by a full-automatic electrochemistry luminescence immunity analyzer. Expression of VEGF, VEGFR-2, MMP-9, and MMP-2 was examined by Western blot and immunocytochemistry. Apoptosis was detected by TUNEL assay. Angiogenesis was detected by in vitro angiogenesis assay kit. Silencing of Alpha-fetoprotein led to an increased apoptosis, which was associated with a decreased expression of vascular endothelial growth factor, vascular endothelial growth factor receptor 2, matrix metalloproteinases-2/9. These results suggest that Alpha-fetoprotein may play a regulatory role on angiogenesis and cell invasion during liver cancer development.
format	article
author	Wenbo Meng Xun Li Zhongtian Bai Yan Li Jinqiu Yuan Tao Liu Jun Yan Wence Zhou Kexiang Zhu Hui Zhang Yumin Li
author_facet	Wenbo Meng Xun Li Zhongtian Bai Yan Li Jinqiu Yuan Tao Liu Jun Yan Wence Zhou Kexiang Zhu Hui Zhang Yumin Li
author_sort	Wenbo Meng
title	Silencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.
title_short	Silencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.
title_full	Silencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.
title_fullStr	Silencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.
title_full_unstemmed	Silencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.
title_sort	silencing alpha-fetoprotein inhibits vegf and mmp-2/9 production in human hepatocellular carcinoma cell.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7accb7d77ea44239b687b2ea9e242ddf
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Silencing alpha-fetoprotein inhibits VEGF and MMP-2/9 production in human hepatocellular carcinoma cell.

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