Examination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia

Early-onset preeclampsia is a common obstetrical disease with a potential genetic background and is characterized by the predominance of Th1 immune response. However, although many studies investigated the immunological environment in preeclamptic patients, no information is available about the pote...

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Autores principales:	Laszlo Szereday, David U. Nagy, Beata Csiszar, Dora Kevey, Timoteus Feik, Matyas Meggyes
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	preeclampsia immune checkpoint TIGIT CD226 CD112 CD155 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7acd391777934af2a855a2b2581bd783
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spelling	oai:doaj.org-article:7acd391777934af2a855a2b2581bd7832021-11-25T16:49:35ZExamination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia10.3390/biomedicines91116082227-9059https://doaj.org/article/7acd391777934af2a855a2b2581bd7832021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1608https://doaj.org/toc/2227-9059Early-onset preeclampsia is a common obstetrical disease with a potential genetic background and is characterized by the predominance of Th1 immune response. However, although many studies investigated the immunological environment in preeclamptic patients, no information is available about the potential role of the TIGIT/CD226/CD112/CD155 immune checkpoint pathway. A total of 37 pregnant women diagnosed with early-onset preeclampsia and 36 control women with appropriately matched gestational age were enrolled in this study. From venous blood, mononuclear cells were isolated and stored in the freezer. Using multicolor flow cytometry T-, NK cell and monocyte subpopulations were determined. After characterization of the immune cell subsets, TIGIT, CD226, CD112, and CD155 surface expression and intracellular granzyme B content were determined by flow cytometer. Significantly decreased CD226 expression and increased CD112 and CD155 surface expression were detected in almost all investigated T-cell, NK cell, and monocyte subpopulations in women diagnosed with preeclampsia compared to the healthy group. Furthermore, reduced TIGIT and granzyme B expression were measured only in preeclamptic CD8+ T cells compared to healthy pregnant women. A decreased level of the activatory receptor CD226 in effector lymphocytes accompanied with an elevated surface presence of the CD112 and CD155 ligands in monocytes could promote the TIGIT/CD112 and/or TIGIT/CD155 ligation, which mediates inhibitory signals. We assume that the inhibition of the immune response via this immune checkpoint pathway might contribute to compensate for the Th1 predominance during early-onset preeclampsia.Laszlo SzeredayDavid U. NagyBeata CsiszarDora KeveyTimoteus FeikMatyas MeggyesMDPI AGarticlepreeclampsiaimmune checkpointTIGITCD226CD112CD155Biology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1608, p 1608 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	preeclampsia immune checkpoint TIGIT CD226 CD112 CD155 Biology (General) QH301-705.5
spellingShingle	preeclampsia immune checkpoint TIGIT CD226 CD112 CD155 Biology (General) QH301-705.5 Laszlo Szereday David U. Nagy Beata Csiszar Dora Kevey Timoteus Feik Matyas Meggyes Examination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia
description	Early-onset preeclampsia is a common obstetrical disease with a potential genetic background and is characterized by the predominance of Th1 immune response. However, although many studies investigated the immunological environment in preeclamptic patients, no information is available about the potential role of the TIGIT/CD226/CD112/CD155 immune checkpoint pathway. A total of 37 pregnant women diagnosed with early-onset preeclampsia and 36 control women with appropriately matched gestational age were enrolled in this study. From venous blood, mononuclear cells were isolated and stored in the freezer. Using multicolor flow cytometry T-, NK cell and monocyte subpopulations were determined. After characterization of the immune cell subsets, TIGIT, CD226, CD112, and CD155 surface expression and intracellular granzyme B content were determined by flow cytometer. Significantly decreased CD226 expression and increased CD112 and CD155 surface expression were detected in almost all investigated T-cell, NK cell, and monocyte subpopulations in women diagnosed with preeclampsia compared to the healthy group. Furthermore, reduced TIGIT and granzyme B expression were measured only in preeclamptic CD8+ T cells compared to healthy pregnant women. A decreased level of the activatory receptor CD226 in effector lymphocytes accompanied with an elevated surface presence of the CD112 and CD155 ligands in monocytes could promote the TIGIT/CD112 and/or TIGIT/CD155 ligation, which mediates inhibitory signals. We assume that the inhibition of the immune response via this immune checkpoint pathway might contribute to compensate for the Th1 predominance during early-onset preeclampsia.
format	article
author	Laszlo Szereday David U. Nagy Beata Csiszar Dora Kevey Timoteus Feik Matyas Meggyes
author_facet	Laszlo Szereday David U. Nagy Beata Csiszar Dora Kevey Timoteus Feik Matyas Meggyes
author_sort	Laszlo Szereday
title	Examination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia
title_short	Examination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia
title_full	Examination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia
title_fullStr	Examination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia
title_full_unstemmed	Examination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia
title_sort	examination of the tigit, cd226, cd112, and cd155 immune checkpoint molecules in peripheral blood mononuclear cells in women diagnosed with early-onset preeclampsia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7acd391777934af2a855a2b2581bd783
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Examination of the TIGIT, CD226, CD112, and CD155 Immune Checkpoint Molecules in Peripheral Blood Mononuclear Cells in Women Diagnosed with Early-Onset Preeclampsia

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