Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.

Iron-oxide based contrast agents play an important role in magnetic resonance imaging (MRI) of labelled cells in vivo. Currently, a wide range of such contrast agents is available with sizes varying from several nanometers up to a few micrometers and consisting of single or multiple magnetic cores....

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Autores principales:	Arthur Taylor, Anne Herrmann, Diana Moss, Violaine Sée, Karen Davies, Steve R Williams, Patricia Murray
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7adbfa2f666f468ca1945868906d7eaf
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spelling	oai:doaj.org-article:7adbfa2f666f468ca1945868906d7eaf2021-11-11T08:21:49ZAssessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.1932-620310.1371/journal.pone.0100259https://doaj.org/article/7adbfa2f666f468ca1945868906d7eaf2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24959883/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Iron-oxide based contrast agents play an important role in magnetic resonance imaging (MRI) of labelled cells in vivo. Currently, a wide range of such contrast agents is available with sizes varying from several nanometers up to a few micrometers and consisting of single or multiple magnetic cores. Here, we evaluate the effectiveness of these different particles for labelling and imaging stem cells, using a mouse mesenchymal stem cell line to investigate intracellular uptake, retention and processing of nano- and microsized contrast agents. The effect of intracellular confinement on transverse relaxivity was measured by MRI at 7 T and in compliance with the principles of the '3Rs', the suitability of the contrast agents for MR-based cell tracking in vivo was tested using a chick embryo model. We show that for all particles tested, relaxivity was markedly reduced following cellular internalisation, indicating that contrast agent relaxivity in colloidal suspension does not accurately predict performance in MR-based cell tracking studies. Using a bimodal imaging approach comprising fluorescence and MRI, we demonstrate that labelled MSC remain viable following in vivo transplantation and can be tracked effectively using MRI. Importantly, our data suggest that larger particles might confer advantages for longer-term imaging.Arthur TaylorAnne HerrmannDiana MossViolaine SéeKaren DaviesSteve R WilliamsPatricia MurrayPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100259 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arthur Taylor Anne Herrmann Diana Moss Violaine Sée Karen Davies Steve R Williams Patricia Murray Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.
description	Iron-oxide based contrast agents play an important role in magnetic resonance imaging (MRI) of labelled cells in vivo. Currently, a wide range of such contrast agents is available with sizes varying from several nanometers up to a few micrometers and consisting of single or multiple magnetic cores. Here, we evaluate the effectiveness of these different particles for labelling and imaging stem cells, using a mouse mesenchymal stem cell line to investigate intracellular uptake, retention and processing of nano- and microsized contrast agents. The effect of intracellular confinement on transverse relaxivity was measured by MRI at 7 T and in compliance with the principles of the '3Rs', the suitability of the contrast agents for MR-based cell tracking in vivo was tested using a chick embryo model. We show that for all particles tested, relaxivity was markedly reduced following cellular internalisation, indicating that contrast agent relaxivity in colloidal suspension does not accurately predict performance in MR-based cell tracking studies. Using a bimodal imaging approach comprising fluorescence and MRI, we demonstrate that labelled MSC remain viable following in vivo transplantation and can be tracked effectively using MRI. Importantly, our data suggest that larger particles might confer advantages for longer-term imaging.
format	article
author	Arthur Taylor Anne Herrmann Diana Moss Violaine Sée Karen Davies Steve R Williams Patricia Murray
author_facet	Arthur Taylor Anne Herrmann Diana Moss Violaine Sée Karen Davies Steve R Williams Patricia Murray
author_sort	Arthur Taylor
title	Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.
title_short	Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.
title_full	Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.
title_fullStr	Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.
title_full_unstemmed	Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.
title_sort	assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for mri cell tracking.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7adbfa2f666f468ca1945868906d7eaf
work_keys_str_mv	AT arthurtaylor assessingtheefficacyofnanoandmicrosizedmagneticparticlesascontrastagentsformricelltracking AT anneherrmann assessingtheefficacyofnanoandmicrosizedmagneticparticlesascontrastagentsformricelltracking AT dianamoss assessingtheefficacyofnanoandmicrosizedmagneticparticlesascontrastagentsformricelltracking AT violainesee assessingtheefficacyofnanoandmicrosizedmagneticparticlesascontrastagentsformricelltracking AT karendavies assessingtheefficacyofnanoandmicrosizedmagneticparticlesascontrastagentsformricelltracking AT steverwilliams assessingtheefficacyofnanoandmicrosizedmagneticparticlesascontrastagentsformricelltracking AT patriciamurray assessingtheefficacyofnanoandmicrosizedmagneticparticlesascontrastagentsformricelltracking
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Assessing the efficacy of nano- and micro-sized magnetic particles as contrast agents for MRI cell tracking.

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