An Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH

ABSTRACT In eukaryotic microbes, little is known about signals that inhibit the proliferation of the cells that secrete the signal, and little is known about signals (chemorepellents) that cause cells to move away from the source of the signal. Autocrine proliferation repressor protein A (AprA) is a...

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Autores principales:	Yu Tang, Yuantai Wu, Sarah E. Herlihy, Francisco J. Brito-Aleman, Jose H. Ting, Chris Janetopoulos, Richard H. Gomer
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	cell density sensing chemorepulsion Dictyostelium G-protein-coupled receptors cell proliferation quorum sensing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7addfcf8d05948fa8ec34887df7d8be0
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spelling	oai:doaj.org-article:7addfcf8d05948fa8ec34887df7d8be02021-11-15T15:53:25ZAn Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH10.1128/mBio.02443-172150-7511https://doaj.org/article/7addfcf8d05948fa8ec34887df7d8be02018-03-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02443-17https://doaj.org/toc/2150-7511ABSTRACT In eukaryotic microbes, little is known about signals that inhibit the proliferation of the cells that secrete the signal, and little is known about signals (chemorepellents) that cause cells to move away from the source of the signal. Autocrine proliferation repressor protein A (AprA) is a protein secreted by the eukaryotic microbe Dictyostelium discoideum. AprA is a chemorepellent for and inhibits the proliferation of D. discoideum. We previously found that cells sense AprA using G proteins, suggesting the existence of a G protein-coupled AprA receptor. To identify the AprA receptor, we screened mutants lacking putative G protein-coupled receptors. We found that, compared to the wild-type strain, cells lacking putative receptor GrlH (grlH¯ cells) show rapid proliferation, do not have large numbers of cells moving away from the edges of colonies, are insensitive to AprA-induced proliferation inhibition and chemorepulsion, and have decreased AprA binding. Expression of GrlH in grlH¯ cells (grlH¯/grlHOE) rescues the phenotypes described above. These data indicate that AprA signaling may be mediated by GrlH in D. discoideum. IMPORTANCE Little is known about how eukaryotic cells can count themselves and thus regulate the size of a tissue or density of cells. In addition, little is known about how eukaryotic cells can sense a repellant signal and move away from the source of the repellant, for instance, to organize the movement of cells in a developing embryo or to move immune cells out of a tissue. In this study, we found that a eukaryotic microbe uses G protein-coupled receptors to mediate both cell density sensing and chemorepulsion.Yu TangYuantai WuSarah E. HerlihyFrancisco J. Brito-AlemanJose H. TingChris JanetopoulosRichard H. GomerAmerican Society for Microbiologyarticlecell density sensingchemorepulsionDictyosteliumG-protein-coupled receptorscell proliferationquorum sensingMicrobiologyQR1-502ENmBio, Vol 9, Iss 1 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	cell density sensing chemorepulsion Dictyostelium G-protein-coupled receptors cell proliferation quorum sensing Microbiology QR1-502
spellingShingle	cell density sensing chemorepulsion Dictyostelium G-protein-coupled receptors cell proliferation quorum sensing Microbiology QR1-502 Yu Tang Yuantai Wu Sarah E. Herlihy Francisco J. Brito-Aleman Jose H. Ting Chris Janetopoulos Richard H. Gomer An Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH
description	ABSTRACT In eukaryotic microbes, little is known about signals that inhibit the proliferation of the cells that secrete the signal, and little is known about signals (chemorepellents) that cause cells to move away from the source of the signal. Autocrine proliferation repressor protein A (AprA) is a protein secreted by the eukaryotic microbe Dictyostelium discoideum. AprA is a chemorepellent for and inhibits the proliferation of D. discoideum. We previously found that cells sense AprA using G proteins, suggesting the existence of a G protein-coupled AprA receptor. To identify the AprA receptor, we screened mutants lacking putative G protein-coupled receptors. We found that, compared to the wild-type strain, cells lacking putative receptor GrlH (grlH¯ cells) show rapid proliferation, do not have large numbers of cells moving away from the edges of colonies, are insensitive to AprA-induced proliferation inhibition and chemorepulsion, and have decreased AprA binding. Expression of GrlH in grlH¯ cells (grlH¯/grlHOE) rescues the phenotypes described above. These data indicate that AprA signaling may be mediated by GrlH in D. discoideum. IMPORTANCE Little is known about how eukaryotic cells can count themselves and thus regulate the size of a tissue or density of cells. In addition, little is known about how eukaryotic cells can sense a repellant signal and move away from the source of the repellant, for instance, to organize the movement of cells in a developing embryo or to move immune cells out of a tissue. In this study, we found that a eukaryotic microbe uses G protein-coupled receptors to mediate both cell density sensing and chemorepulsion.
format	article
author	Yu Tang Yuantai Wu Sarah E. Herlihy Francisco J. Brito-Aleman Jose H. Ting Chris Janetopoulos Richard H. Gomer
author_facet	Yu Tang Yuantai Wu Sarah E. Herlihy Francisco J. Brito-Aleman Jose H. Ting Chris Janetopoulos Richard H. Gomer
author_sort	Yu Tang
title	An Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH
title_short	An Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH
title_full	An Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH
title_fullStr	An Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH
title_full_unstemmed	An Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH
title_sort	autocrine proliferation repressor regulates <italic toggle="yes">dictyostelium discoideum</italic> proliferation and chemorepulsion using the g protein-coupled receptor grlh
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/7addfcf8d05948fa8ec34887df7d8be0
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An Autocrine Proliferation Repressor Regulates <italic toggle="yes">Dictyostelium discoideum</italic> Proliferation and Chemorepulsion Using the G Protein-Coupled Receptor GrlH

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