Identification of potential transcription factors that enhance human iPSC generation

Abstract Although many factors have been identified and used to enhance the iPSC reprogramming process, its efficiency remains quite low. In addition, reprogramming efficacy has been evidenced to be affected by disease mutations that are present in patient samples. In this study, using RNA-seq platf...

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Autores principales:	Nuha T. Swaidan, Salam Salloum-Asfar, Freshteh Palangi, Khaoula Errafii, Nada H. Soliman, Ahmed T. Aboughalia, Abdul Haseeb S. Wali, Sara A. Abdulla, Mohamed M. Emara
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7ae603a437084f20abc470cc1eac7cb6
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spelling	oai:doaj.org-article:7ae603a437084f20abc470cc1eac7cb62021-12-02T12:40:41ZIdentification of potential transcription factors that enhance human iPSC generation10.1038/s41598-020-78932-92045-2322https://doaj.org/article/7ae603a437084f20abc470cc1eac7cb62020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-78932-9https://doaj.org/toc/2045-2322Abstract Although many factors have been identified and used to enhance the iPSC reprogramming process, its efficiency remains quite low. In addition, reprogramming efficacy has been evidenced to be affected by disease mutations that are present in patient samples. In this study, using RNA-seq platform we have identified and validated the differential gene expression of five transcription factors (TFs) (GBX2, NANOGP8, SP8, PEG3, and ZIC1) that were associated with a remarkable increase in the number of iPSC colonies generated from a patient with Parkinson's disease. We have applied different bioinformatics tools (Gene ontology, protein–protein interaction, and signaling pathways analyses) to investigate the possible roles of these TFs in pluripotency and developmental process. Interestingly, GBX2, NANOGP8, SP8, PEG3, and ZIC1 were found to play a role in maintaining pluripotency, regulating self-renewal stages, and interacting with other factors that are involved in pluripotency regulation including OCT4, SOX2, NANOG, and KLF4. Therefore, the TFs identified in this study could be used as additional transcription factors that enhance reprogramming efficiency to boost iPSC generation technology.Nuha T. SwaidanSalam Salloum-AsfarFreshteh PalangiKhaoula ErrafiiNada H. SolimanAhmed T. AboughaliaAbdul Haseeb S. WaliSara A. AbdullaMohamed M. EmaraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-19 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nuha T. Swaidan Salam Salloum-Asfar Freshteh Palangi Khaoula Errafii Nada H. Soliman Ahmed T. Aboughalia Abdul Haseeb S. Wali Sara A. Abdulla Mohamed M. Emara Identification of potential transcription factors that enhance human iPSC generation
description	Abstract Although many factors have been identified and used to enhance the iPSC reprogramming process, its efficiency remains quite low. In addition, reprogramming efficacy has been evidenced to be affected by disease mutations that are present in patient samples. In this study, using RNA-seq platform we have identified and validated the differential gene expression of five transcription factors (TFs) (GBX2, NANOGP8, SP8, PEG3, and ZIC1) that were associated with a remarkable increase in the number of iPSC colonies generated from a patient with Parkinson's disease. We have applied different bioinformatics tools (Gene ontology, protein–protein interaction, and signaling pathways analyses) to investigate the possible roles of these TFs in pluripotency and developmental process. Interestingly, GBX2, NANOGP8, SP8, PEG3, and ZIC1 were found to play a role in maintaining pluripotency, regulating self-renewal stages, and interacting with other factors that are involved in pluripotency regulation including OCT4, SOX2, NANOG, and KLF4. Therefore, the TFs identified in this study could be used as additional transcription factors that enhance reprogramming efficiency to boost iPSC generation technology.
format	article
author	Nuha T. Swaidan Salam Salloum-Asfar Freshteh Palangi Khaoula Errafii Nada H. Soliman Ahmed T. Aboughalia Abdul Haseeb S. Wali Sara A. Abdulla Mohamed M. Emara
author_facet	Nuha T. Swaidan Salam Salloum-Asfar Freshteh Palangi Khaoula Errafii Nada H. Soliman Ahmed T. Aboughalia Abdul Haseeb S. Wali Sara A. Abdulla Mohamed M. Emara
author_sort	Nuha T. Swaidan
title	Identification of potential transcription factors that enhance human iPSC generation
title_short	Identification of potential transcription factors that enhance human iPSC generation
title_full	Identification of potential transcription factors that enhance human iPSC generation
title_fullStr	Identification of potential transcription factors that enhance human iPSC generation
title_full_unstemmed	Identification of potential transcription factors that enhance human iPSC generation
title_sort	identification of potential transcription factors that enhance human ipsc generation
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/7ae603a437084f20abc470cc1eac7cb6
work_keys_str_mv	AT nuhatswaidan identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration AT salamsalloumasfar identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration AT freshtehpalangi identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration AT khaoulaerrafii identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration AT nadahsoliman identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration AT ahmedtaboughalia identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration AT abdulhaseebswali identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration AT saraaabdulla identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration AT mohamedmemara identificationofpotentialtranscriptionfactorsthatenhancehumanipscgeneration
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Identification of potential transcription factors that enhance human iPSC generation

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