Surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis

Chengchong Ai, Dandan Sheng, Jun Chen, Jiangyu Cai, Siheng Wang, Jia Jiang, Shiyi Chen Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China Abstract: Ultra-high-molecular-weight polyethylene (UHMWPE) has been applied in orthopedics, as t...

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Autores principales:	Ai C, Sheng D, Chen J, Cai J, Wang S, Jiang J, Chen S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	UHMWPE surface modification VEGF silk fibroin graft-bone healing Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7af0410fa3ad4ee69924ba501f1bc834
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spelling	oai:doaj.org-article:7af0410fa3ad4ee69924ba501f1bc8342021-12-02T07:13:43ZSurface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis1178-2013https://doaj.org/article/7af0410fa3ad4ee69924ba501f1bc8342017-10-01T00:00:00Zhttps://www.dovepress.com/surface-modification-of-vascular-endothelial-growth-factor-loaded-silk-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Chengchong Ai, Dandan Sheng, Jun Chen, Jiangyu Cai, Siheng Wang, Jia Jiang, Shiyi Chen Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China Abstract: Ultra-high-molecular-weight polyethylene (UHMWPE) has been applied in orthopedics, as the materials of joint prosthesis, artificial ligaments, and sutures due to its advantages such as high tensile strength, good wear resistance, and chemical stability. However, postoperative osteolysis induced by UHMWPE wear particles and poor bone–implant healing interface due to scarcity of osseointegration is a significant problem and should be solved imperatively. In order to enhance its affinity to bone tissue, vascular endothelial growth factor (VEGF) was loaded on the surface of materials, the loading was performed by silk fibroin (SF) coating to achieve a controlled-release delivery. Several techniques including field emission scanning electron microscopy (FESEM) and attenuated total reflectance (ATR)-Fourier transform infrared (FTIR) and water contact angle measurement were used to validate the effectiveness of introduction of SF/VEGF. The result of ELISA demonstrated that the release of VEGF was well maintained up to 4 weeks. The modified UHMWPE was evaluated by both in vitro and in vivo experiments. According to the results of FESEM and cell counting kit-8 (CCK-8) assay, bone marrow mesenchymal stem cells cultured on the UHMWPE coated with SF/VEGF and SF exhibited a better proliferation performance than that of the pristine UHMWPE. The model rabbit of anterior cruciate ligament reconstruction was used to observe the graft–bone healing process in vivo. The results of histological evaluation, microcomputed tomography (micro-CT) analysis, and biomechanical tests performed at 6 and 12 weeks after surgery demonstrated that graft–bone healing could be significantly improved due to the effect of VEGF on angiogenesis, which was loaded on the surface by SF coating. This study showed that the method loading VEGF on UHMWPE by SF coating played an effective role on the biological performance of UHMWPE and displayed a great potential application for anterior cruciate ligament reconstruction. Keywords: UHMWPE, surface modification, VEGF, silk fibroin, graft–bone healingAi CSheng DChen JCai JWang SJiang JChen SDove Medical PressarticleUHMWPEsurface modificationVEGFsilk fibroingraft-bone healingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7737-7750 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	UHMWPE surface modification VEGF silk fibroin graft-bone healing Medicine (General) R5-920
spellingShingle	UHMWPE surface modification VEGF silk fibroin graft-bone healing Medicine (General) R5-920 Ai C Sheng D Chen J Cai J Wang S Jiang J Chen S Surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis
description	Chengchong Ai, Dandan Sheng, Jun Chen, Jiangyu Cai, Siheng Wang, Jia Jiang, Shiyi Chen Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, People’s Republic of China Abstract: Ultra-high-molecular-weight polyethylene (UHMWPE) has been applied in orthopedics, as the materials of joint prosthesis, artificial ligaments, and sutures due to its advantages such as high tensile strength, good wear resistance, and chemical stability. However, postoperative osteolysis induced by UHMWPE wear particles and poor bone–implant healing interface due to scarcity of osseointegration is a significant problem and should be solved imperatively. In order to enhance its affinity to bone tissue, vascular endothelial growth factor (VEGF) was loaded on the surface of materials, the loading was performed by silk fibroin (SF) coating to achieve a controlled-release delivery. Several techniques including field emission scanning electron microscopy (FESEM) and attenuated total reflectance (ATR)-Fourier transform infrared (FTIR) and water contact angle measurement were used to validate the effectiveness of introduction of SF/VEGF. The result of ELISA demonstrated that the release of VEGF was well maintained up to 4 weeks. The modified UHMWPE was evaluated by both in vitro and in vivo experiments. According to the results of FESEM and cell counting kit-8 (CCK-8) assay, bone marrow mesenchymal stem cells cultured on the UHMWPE coated with SF/VEGF and SF exhibited a better proliferation performance than that of the pristine UHMWPE. The model rabbit of anterior cruciate ligament reconstruction was used to observe the graft–bone healing process in vivo. The results of histological evaluation, microcomputed tomography (micro-CT) analysis, and biomechanical tests performed at 6 and 12 weeks after surgery demonstrated that graft–bone healing could be significantly improved due to the effect of VEGF on angiogenesis, which was loaded on the surface by SF coating. This study showed that the method loading VEGF on UHMWPE by SF coating played an effective role on the biological performance of UHMWPE and displayed a great potential application for anterior cruciate ligament reconstruction. Keywords: UHMWPE, surface modification, VEGF, silk fibroin, graft–bone healing
format	article
author	Ai C Sheng D Chen J Cai J Wang S Jiang J Chen S
author_facet	Ai C Sheng D Chen J Cai J Wang S Jiang J Chen S
author_sort	Ai C
title	Surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis
title_short	Surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis
title_full	Surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis
title_fullStr	Surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis
title_full_unstemmed	Surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis
title_sort	surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/7af0410fa3ad4ee69924ba501f1bc834
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Surface modification of vascular endothelial growth factor-loaded silk fibroin to improve biological performance of ultra-high-molecular-weight polyethylene via promoting angiogenesis

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