Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration

Probiotic administration is a potential strategy against enteric pathogen infection in either clinical treatment or animal nutrition industry, but the administration duration of probiotics varied and the underlying mechanisms remain unclear. A strain (YC) affiliated to Pediococcus pentosaceus, a com...

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Autores principales:	Chengjie Shan, Miao Li, Zhu Liu, Rong Xu, Fang Qiao, Zhen-Yu Du, Mei-Ling Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	probiotic pathogen resistance administration duration butyrate NLRP3 inflammasome Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/7b066457684a4aab91788b76a2d7ac84
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spelling	oai:doaj.org-article:7b066457684a4aab91788b76a2d7ac842021-12-01T07:44:16ZPediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration1664-322410.3389/fimmu.2021.766401https://doaj.org/article/7b066457684a4aab91788b76a2d7ac842021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fimmu.2021.766401/fullhttps://doaj.org/toc/1664-3224Probiotic administration is a potential strategy against enteric pathogen infection in either clinical treatment or animal nutrition industry, but the administration duration of probiotics varied and the underlying mechanisms remain unclear. A strain (YC) affiliated to Pediococcus pentosaceus, a commonly used probiotic, was isolated from fish gut and the potential role of YC against Aeromonas hydrophila was detected in zebrafish. We found that 3- or 4-week YC administration (YC3W or YC4W) increased the resistance against A. hydrophila while 1- or 2-week treatment (YC1W or YC2W) did not. To determine the possible reason, intestinal microbiota analysis and RNAseq were conducted. The results showed that compared with CON and YC1W, YC4W significantly increased the abundance of short-chain fatty acids (SCFAs) producing bacteria and elevated the gene expression of nlrp3. Higher butyrate content and enhanced expression of IL1β were subsequently found in YC4W. To identify the causal relationship between butyrate and the higher pathogen resistance, different concentrations of sodium butyrate (SB) were supplemented. The results suggested that 10 mmol/kg SB addition mirrored the protective effect of YC4W by increasing the production of IL-1β. Furthermore, the increased IL-1β raised the percentage of intestinal neutrophils, which endued the zebrafish with A. hydrophila resistance. In vivo knockdown of intestinal il1b eliminated the anti-infection effect. Collectively, our data suggested that the molecular mechanism of probiotics determined the administration duration, which is vital for the efficiency of probiotics. Promoting host inflammation by probiotic pretreatment is one potential way for probiotics to provide their protective effects against pathogens.Chengjie ShanMiao LiZhu LiuZhu LiuRong XuFang QiaoZhen-Yu DuMei-Ling ZhangFrontiers Media S.A.articleprobioticpathogen resistanceadministration durationbutyrateNLRP3 inflammasomeImmunologic diseases. AllergyRC581-607ENFrontiers in Immunology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	probiotic pathogen resistance administration duration butyrate NLRP3 inflammasome Immunologic diseases. Allergy RC581-607
spellingShingle	probiotic pathogen resistance administration duration butyrate NLRP3 inflammasome Immunologic diseases. Allergy RC581-607 Chengjie Shan Miao Li Zhu Liu Zhu Liu Rong Xu Fang Qiao Zhen-Yu Du Mei-Ling Zhang Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration
description	Probiotic administration is a potential strategy against enteric pathogen infection in either clinical treatment or animal nutrition industry, but the administration duration of probiotics varied and the underlying mechanisms remain unclear. A strain (YC) affiliated to Pediococcus pentosaceus, a commonly used probiotic, was isolated from fish gut and the potential role of YC against Aeromonas hydrophila was detected in zebrafish. We found that 3- or 4-week YC administration (YC3W or YC4W) increased the resistance against A. hydrophila while 1- or 2-week treatment (YC1W or YC2W) did not. To determine the possible reason, intestinal microbiota analysis and RNAseq were conducted. The results showed that compared with CON and YC1W, YC4W significantly increased the abundance of short-chain fatty acids (SCFAs) producing bacteria and elevated the gene expression of nlrp3. Higher butyrate content and enhanced expression of IL1β were subsequently found in YC4W. To identify the causal relationship between butyrate and the higher pathogen resistance, different concentrations of sodium butyrate (SB) were supplemented. The results suggested that 10 mmol/kg SB addition mirrored the protective effect of YC4W by increasing the production of IL-1β. Furthermore, the increased IL-1β raised the percentage of intestinal neutrophils, which endued the zebrafish with A. hydrophila resistance. In vivo knockdown of intestinal il1b eliminated the anti-infection effect. Collectively, our data suggested that the molecular mechanism of probiotics determined the administration duration, which is vital for the efficiency of probiotics. Promoting host inflammation by probiotic pretreatment is one potential way for probiotics to provide their protective effects against pathogens.
format	article
author	Chengjie Shan Miao Li Zhu Liu Zhu Liu Rong Xu Fang Qiao Zhen-Yu Du Mei-Ling Zhang
author_facet	Chengjie Shan Miao Li Zhu Liu Zhu Liu Rong Xu Fang Qiao Zhen-Yu Du Mei-Ling Zhang
author_sort	Chengjie Shan
title	Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration
title_short	Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration
title_full	Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration
title_fullStr	Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration
title_full_unstemmed	Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration
title_sort	pediococcus pentosaceus enhances host resistance against pathogen by increasing il-1β production: understanding probiotic effectiveness and administration duration
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/7b066457684a4aab91788b76a2d7ac84
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Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration

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