Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

Abstract. Suranto, Wahyuni D, Edy Purwanto E. 2019. Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia. Biodiversitas 20: 2511-2518. Sweet potato (Ipomoea batatas Lam) has been recorded to have many cultivars an...

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Autores principales:	Suranto Suranto, DWI WAHYUNI, EDY PURWANTO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	cultivar, ipomoea batatas, peroxidase, pollen features Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7b0c0b850f5b4b9e9279e2a7aa0a1830
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spelling	oai:doaj.org-article:7b0c0b850f5b4b9e9279e2a7aa0a18302021-11-21T21:54:26ZEvidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia1412-033X2085-472210.13057/biodiv/d200912https://doaj.org/article/7b0c0b850f5b4b9e9279e2a7aa0a18302019-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3984https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Suranto, Wahyuni D, Edy Purwanto E. 2019. Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia. Biodiversitas 20: 2511-2518. Sweet potato (Ipomoea batatas Lam) has been recorded to have many cultivars and variations in the morphological characters not only for the leaves but also for the tubers. Sweet potatoes had economically significant role, especially as an additional food due to its high content of carbohydrate and other nutrient values. In order to test whether the morphological variation in this species was also expressed at the micro character; such as pollen morphology and enzyme activity, ten sweet potato cultivars were used in this study. The morphological characters such as shape and structure of the pollen features were examined using Scanning Electron Microscopy (SEM). Accordingly, Polyacrylamide Gel Electrophoresis (PAGE) was used to look-at the banding pattern of peroxidase isozymes. Morphological characters of leaves, flowers, and tuber were observed qualitatively both for the typical shape and colors. More detail of flower shapes was drown manually especially their petal edges. The results showed that there was variation among the ten sweet potato cultivars confirmed not only on their morphological characters but also the isozyme banding patterns of ten cultivars tested. At least, two cultivar Antin 3 and Papua Solossa had uniques characters especially the purple stem and yellow tuber and dark skin on the tuber, respectively. In addition, the structural analysis of pollen showed that Beta 1 cultivar has very large pollen; 116,50 ?m (axis polar) and the contour surface of exine pollen was concave with the aperture shape and ornamentation were prolate spheroidal and echinate respectively. This unique character was also shown by its peroxidase isozyme bands. Eleven bands of leaf and 13 bands of stem samples were detected respectively. The typical Rf values of 0.31, 0.48 and 0.41 were detected for the leaf, while for stem samples were 0.14, 0.20 and 0.26. The Beta 1 cultivar showed very thick bands in between Rf values of 0.13 and 0.26. Meanwhile, the unique band pattern of Papua Salosa was the absence of band at 0.38.Suranto SurantoDWI WAHYUNIEDY PURWANTOMBI & UNS Soloarticlecultivar, ipomoea batatas, peroxidase, pollen featuresBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	cultivar, ipomoea batatas, peroxidase, pollen features Biology (General) QH301-705.5
spellingShingle	cultivar, ipomoea batatas, peroxidase, pollen features Biology (General) QH301-705.5 Suranto Suranto DWI WAHYUNI EDY PURWANTO Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia
description	Abstract. Suranto, Wahyuni D, Edy Purwanto E. 2019. Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia. Biodiversitas 20: 2511-2518. Sweet potato (Ipomoea batatas Lam) has been recorded to have many cultivars and variations in the morphological characters not only for the leaves but also for the tubers. Sweet potatoes had economically significant role, especially as an additional food due to its high content of carbohydrate and other nutrient values. In order to test whether the morphological variation in this species was also expressed at the micro character; such as pollen morphology and enzyme activity, ten sweet potato cultivars were used in this study. The morphological characters such as shape and structure of the pollen features were examined using Scanning Electron Microscopy (SEM). Accordingly, Polyacrylamide Gel Electrophoresis (PAGE) was used to look-at the banding pattern of peroxidase isozymes. Morphological characters of leaves, flowers, and tuber were observed qualitatively both for the typical shape and colors. More detail of flower shapes was drown manually especially their petal edges. The results showed that there was variation among the ten sweet potato cultivars confirmed not only on their morphological characters but also the isozyme banding patterns of ten cultivars tested. At least, two cultivar Antin 3 and Papua Solossa had uniques characters especially the purple stem and yellow tuber and dark skin on the tuber, respectively. In addition, the structural analysis of pollen showed that Beta 1 cultivar has very large pollen; 116,50 ?m (axis polar) and the contour surface of exine pollen was concave with the aperture shape and ornamentation were prolate spheroidal and echinate respectively. This unique character was also shown by its peroxidase isozyme bands. Eleven bands of leaf and 13 bands of stem samples were detected respectively. The typical Rf values of 0.31, 0.48 and 0.41 were detected for the leaf, while for stem samples were 0.14, 0.20 and 0.26. The Beta 1 cultivar showed very thick bands in between Rf values of 0.13 and 0.26. Meanwhile, the unique band pattern of Papua Salosa was the absence of band at 0.38.
format	article
author	Suranto Suranto DWI WAHYUNI EDY PURWANTO
author_facet	Suranto Suranto DWI WAHYUNI EDY PURWANTO
author_sort	Suranto Suranto
title	Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia
title_short	Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia
title_full	Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia
title_fullStr	Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia
title_full_unstemmed	Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia
title_sort	evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from indonesia
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/7b0c0b850f5b4b9e9279e2a7aa0a1830
work_keys_str_mv	AT surantosuranto evidenceofpollenfeaturesandperoxidaseisozymesintheirmorphologicalcomplexityoftenlocalcultivarsofsweetpotatofromindonesia AT dwiwahyuni evidenceofpollenfeaturesandperoxidaseisozymesintheirmorphologicalcomplexityoftenlocalcultivarsofsweetpotatofromindonesia AT edypurwanto evidenceofpollenfeaturesandperoxidaseisozymesintheirmorphologicalcomplexityoftenlocalcultivarsofsweetpotatofromindonesia
_version_	1718418650333970432

Evidence of pollen features and peroxidase isozymes in their morphological complexity of ten local cultivars of sweet potato from Indonesia

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