Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations

Abstract The aim of this study was to investigate the hyperacute and acute changes in apparent diffusion coefficient (ADC), T1, and T2 mapping in rat kidneys after severe bilateral renal ischemic-reperfusion injury (IRI). After baseline MRI, 24 Spraque-Dawley rats with renal IRI were divided equally...

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Autores principales:	Sheung-Fat Ko, Hon-Kan Yip, Yen-Yi Zhen, Chia-Chang Lee, Chen-Chang Lee, Shyh-Jer Huang, Chung-Cheng Huang, Shu-Hang Ng, Jui-Wei Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b19cab0fd80420197e50a5733bcc8ca
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spelling	oai:doaj.org-article:7b19cab0fd80420197e50a5733bcc8ca2021-12-02T15:05:38ZSevere bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations10.1038/s41598-017-01895-x2045-2322https://doaj.org/article/7b19cab0fd80420197e50a5733bcc8ca2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01895-xhttps://doaj.org/toc/2045-2322Abstract The aim of this study was to investigate the hyperacute and acute changes in apparent diffusion coefficient (ADC), T1, and T2 mapping in rat kidneys after severe bilateral renal ischemic-reperfusion injury (IRI). After baseline MRI, 24 Spraque-Dawley rats with renal IRI were divided equally as group 1 (post-IRI MRI at 6 hours, days 1, 3, and 7) and groups 2, 3, and 4 (post-IRI MRI at 6 hours; 6 hours and day 1; 6 hours, days 1 and 3, respectively), while six other rats without IRI (group 5) were used as sham control. ADC, T1, and T2 values of the cortex and outer and inner stripes of outer medulla (OSOM and ISOM), and immunohistochemical studies assessing monocyte chemoattractant protein-1 (MCP-1), CD68+ cells, tubular cast formation, and collagen deposition in three zones at different time points were evaluated. Significantly reduced ADCs in OSOM and ISOM are noninvasive biomarkers denoting hyperacute damages after IRI. Linear regression analysis revealed a significant inverse correlation between 6-hour/baseline ADC ratios and MCP-1 staining (P < 0.001, r2 = 0.738). ADC, T1, and T2 values are useful for assessing variable IRI changes in different layers depending on underlying microstructural and histopathological changes at different time points.Sheung-Fat KoHon-Kan YipYen-Yi ZhenChia-Chang LeeChen-Chang LeeShyh-Jer HuangChung-Cheng HuangShu-Hang NgJui-Wei LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sheung-Fat Ko Hon-Kan Yip Yen-Yi Zhen Chia-Chang Lee Chen-Chang Lee Shyh-Jer Huang Chung-Cheng Huang Shu-Hang Ng Jui-Wei Lin Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations
description	Abstract The aim of this study was to investigate the hyperacute and acute changes in apparent diffusion coefficient (ADC), T1, and T2 mapping in rat kidneys after severe bilateral renal ischemic-reperfusion injury (IRI). After baseline MRI, 24 Spraque-Dawley rats with renal IRI were divided equally as group 1 (post-IRI MRI at 6 hours, days 1, 3, and 7) and groups 2, 3, and 4 (post-IRI MRI at 6 hours; 6 hours and day 1; 6 hours, days 1 and 3, respectively), while six other rats without IRI (group 5) were used as sham control. ADC, T1, and T2 values of the cortex and outer and inner stripes of outer medulla (OSOM and ISOM), and immunohistochemical studies assessing monocyte chemoattractant protein-1 (MCP-1), CD68+ cells, tubular cast formation, and collagen deposition in three zones at different time points were evaluated. Significantly reduced ADCs in OSOM and ISOM are noninvasive biomarkers denoting hyperacute damages after IRI. Linear regression analysis revealed a significant inverse correlation between 6-hour/baseline ADC ratios and MCP-1 staining (P < 0.001, r2 = 0.738). ADC, T1, and T2 values are useful for assessing variable IRI changes in different layers depending on underlying microstructural and histopathological changes at different time points.
format	article
author	Sheung-Fat Ko Hon-Kan Yip Yen-Yi Zhen Chia-Chang Lee Chen-Chang Lee Shyh-Jer Huang Chung-Cheng Huang Shu-Hang Ng Jui-Wei Lin
author_facet	Sheung-Fat Ko Hon-Kan Yip Yen-Yi Zhen Chia-Chang Lee Chen-Chang Lee Shyh-Jer Huang Chung-Cheng Huang Shu-Hang Ng Jui-Wei Lin
author_sort	Sheung-Fat Ko
title	Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations
title_short	Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations
title_full	Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations
title_fullStr	Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations
title_full_unstemmed	Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations
title_sort	severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, t1, and t2 mapping with immunohistochemical correlations
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/7b19cab0fd80420197e50a5733bcc8ca
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Severe bilateral ischemic-reperfusion renal injury: hyperacute and acute changes in apparent diffusion coefficient, T1, and T2 mapping with immunohistochemical correlations

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