Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.

Lactobacillus kefiranofaciens M1 is a novel probiotic strain that was isolated from kefir grains. Previously, we have demonstrated the immunoregulatory, anti-allergic, anti-asthmatic and anti-colitis abilities of L. kefiranofaciens M1 in a number of in-vitro and in-vivo experiments. However, whether...

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Autores principales:	Yen-Po Chen, Ming-Ju Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b1f6c057a9040d2b46d7f71f06db37a
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spelling	oai:doaj.org-article:7b1f6c057a9040d2b46d7f71f06db37a2021-11-18T08:47:32ZEffects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.1932-620310.1371/journal.pone.0078789https://doaj.org/article/7b1f6c057a9040d2b46d7f71f06db37a2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24244362/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Lactobacillus kefiranofaciens M1 is a novel probiotic strain that was isolated from kefir grains. Previously, we have demonstrated the immunoregulatory, anti-allergic, anti-asthmatic and anti-colitis abilities of L. kefiranofaciens M1 in a number of in-vitro and in-vivo experiments. However, whether the effects of L. kefiranofaciens M1 are elicited directly on the host or act by regulating the host's microbiota remains unknown. A number of studies have used germ-free or gnotobiotic animals to investigate the relationship between probiotics and colitis; therefore the aim of this study was to investigate the effects of L. kefiranofaciens M1 on germ-free mice. Such an approach should help in determining the direct effects of L. kefiranofaciens M1 on the host itself. Four-week-old female germ-free mice were inoculated intragastrically with 2×10(8) CFU/mouse L. kefiranofaciens M1 once or at 2-day intervals for 14 days. Bacterial colonization, the Th1/Th2 cytokine profile of the mice's splenocytes and the anti-colitis effect of L. kefiranofaciens M1 were investigated. The strongest response in terms of splenic Th1 cytokine IFN-γ and IL-12 production upon TLR activation was detected in the continuous treatment group when comparing to the single inoculation group and the germ-free control. In addition, continuous inoculation with L. kefiranofaciens M1 was found to ameliorate the symptoms of DSS-induced colitis in germ-free mice. However, L. kefiranofaciens M1 failed to colonize the host. Thus it would seem that L. kefiranofaciens M1 is likely to act directly on the host and not be involved in microbiota regulation.Yen-Po ChenMing-Ju ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e78789 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yen-Po Chen Ming-Ju Chen Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.
description	Lactobacillus kefiranofaciens M1 is a novel probiotic strain that was isolated from kefir grains. Previously, we have demonstrated the immunoregulatory, anti-allergic, anti-asthmatic and anti-colitis abilities of L. kefiranofaciens M1 in a number of in-vitro and in-vivo experiments. However, whether the effects of L. kefiranofaciens M1 are elicited directly on the host or act by regulating the host's microbiota remains unknown. A number of studies have used germ-free or gnotobiotic animals to investigate the relationship between probiotics and colitis; therefore the aim of this study was to investigate the effects of L. kefiranofaciens M1 on germ-free mice. Such an approach should help in determining the direct effects of L. kefiranofaciens M1 on the host itself. Four-week-old female germ-free mice were inoculated intragastrically with 2×10(8) CFU/mouse L. kefiranofaciens M1 once or at 2-day intervals for 14 days. Bacterial colonization, the Th1/Th2 cytokine profile of the mice's splenocytes and the anti-colitis effect of L. kefiranofaciens M1 were investigated. The strongest response in terms of splenic Th1 cytokine IFN-γ and IL-12 production upon TLR activation was detected in the continuous treatment group when comparing to the single inoculation group and the germ-free control. In addition, continuous inoculation with L. kefiranofaciens M1 was found to ameliorate the symptoms of DSS-induced colitis in germ-free mice. However, L. kefiranofaciens M1 failed to colonize the host. Thus it would seem that L. kefiranofaciens M1 is likely to act directly on the host and not be involved in microbiota regulation.
format	article
author	Yen-Po Chen Ming-Ju Chen
author_facet	Yen-Po Chen Ming-Ju Chen
author_sort	Yen-Po Chen
title	Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.
title_short	Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.
title_full	Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.
title_fullStr	Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.
title_full_unstemmed	Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.
title_sort	effects of lactobacillus kefiranofaciens m1 isolated from kefir grains on germ-free mice.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/7b1f6c057a9040d2b46d7f71f06db37a
work_keys_str_mv	AT yenpochen effectsoflactobacilluskefiranofaciensm1isolatedfromkefirgrainsongermfreemice AT mingjuchen effectsoflactobacilluskefiranofaciensm1isolatedfromkefirgrainsongermfreemice
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Effects of Lactobacillus kefiranofaciens M1 isolated from kefir grains on germ-free mice.

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