A magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field

Zhongyang Liu,1,* Shu Zhu,1,* Liang Liu,2,* Jun Ge,3,4,* Liangliang Huang,1 Zhen Sun,1 Wen Zeng,5 Jinghui Huang,1 Zhuojing Luo1 1Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, 2Department of Orthopedics, No 161 Hospital of PLA, Wuh...

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Autores principales:	Liu ZY, Zhu S, Liu L, Ge J, Huang LL, Sun Z, Zeng W, Huang JH, Luo ZJ
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	magnetic nanoparticle nanocomposite magnetic field Schwann cell nerve regeneration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7b204bbc33784834a07f34955e971865
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spelling	oai:doaj.org-article:7b204bbc33784834a07f34955e9718652021-12-02T03:11:42ZA magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field1178-2013https://doaj.org/article/7b204bbc33784834a07f34955e9718652017-10-01T00:00:00Zhttps://www.dovepress.com/a-magnetically-responsive-nanocomposite-scaffold-combined-with-schwann-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhongyang Liu,1,* Shu Zhu,1,* Liang Liu,2,* Jun Ge,3,4,* Liangliang Huang,1 Zhen Sun,1 Wen Zeng,5 Jinghui Huang,1 Zhuojing Luo1 1Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, 2Department of Orthopedics, No 161 Hospital of PLA, Wuhan, Hubei, 3Department of Orthopedics, No 323 Hospital of PLA, Xi’an, Shaanxi, 4Department of Anatomy, Fourth Military Medical University, Xi’an, Shaanxi, 5Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China *These authors contributed equally to this work Abstract: Peripheral nerve repair is still challenging for surgeons. Autologous nerve transplantation is the acknowledged therapy; however, its application is limited by the scarcity of available donor nerves, donor area morbidity, and neuroma formation. Biomaterials for engineering artificial nerves, particularly materials combined with supportive cells, display remarkable promising prospects. Schwann cells (SCs) are the absorbing seeding cells in peripheral nerve engineering repair; however, the attenuated biologic activity restricts their application. In this study, a magnetic nanocomposite scaffold fabricated from magnetic nanoparticles and a biodegradable chitosan–glycerophosphate polymer was made. Its structure was evaluated and characterized. The combined effects of magnetic scaffold (MG) with an applied magnetic field (MF) on the viability of SCs and peripheral nerve injury repair were investigated. The magnetic nanocomposite scaffold showed tunable magnetization and degradation rate. The MGs synergized with the applied MF to enhance the viability of SCs after transplantation. Furthermore, nerve regeneration and functional recovery were promoted by the synergism of SCs-loaded MGs and MF. Based on the current findings, the combined application of MGs and SCs with applied MF is a promising therapy for the engineering of peripheral nerve regeneration. Keywords: peripheral nerve repair, magnetic nanoparticle, nanocomposite, magnetic field, Schwann cell, functional recoveryLiu ZYZhu SLiu LGe JHuang LLSun ZZeng WHuang JHLuo ZJDove Medical Pressarticlemagnetic nanoparticlenanocompositemagnetic fieldSchwann cellnerve regenerationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7815-7832 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	magnetic nanoparticle nanocomposite magnetic field Schwann cell nerve regeneration Medicine (General) R5-920
spellingShingle	magnetic nanoparticle nanocomposite magnetic field Schwann cell nerve regeneration Medicine (General) R5-920 Liu ZY Zhu S Liu L Ge J Huang LL Sun Z Zeng W Huang JH Luo ZJ A magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field
description	Zhongyang Liu,1,* Shu Zhu,1,* Liang Liu,2,* Jun Ge,3,4,* Liangliang Huang,1 Zhen Sun,1 Wen Zeng,5 Jinghui Huang,1 Zhuojing Luo1 1Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Xi’an, Shaanxi, 2Department of Orthopedics, No 161 Hospital of PLA, Wuhan, Hubei, 3Department of Orthopedics, No 323 Hospital of PLA, Xi’an, Shaanxi, 4Department of Anatomy, Fourth Military Medical University, Xi’an, Shaanxi, 5Department of Neurosurgery, Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi, People’s Republic of China *These authors contributed equally to this work Abstract: Peripheral nerve repair is still challenging for surgeons. Autologous nerve transplantation is the acknowledged therapy; however, its application is limited by the scarcity of available donor nerves, donor area morbidity, and neuroma formation. Biomaterials for engineering artificial nerves, particularly materials combined with supportive cells, display remarkable promising prospects. Schwann cells (SCs) are the absorbing seeding cells in peripheral nerve engineering repair; however, the attenuated biologic activity restricts their application. In this study, a magnetic nanocomposite scaffold fabricated from magnetic nanoparticles and a biodegradable chitosan–glycerophosphate polymer was made. Its structure was evaluated and characterized. The combined effects of magnetic scaffold (MG) with an applied magnetic field (MF) on the viability of SCs and peripheral nerve injury repair were investigated. The magnetic nanocomposite scaffold showed tunable magnetization and degradation rate. The MGs synergized with the applied MF to enhance the viability of SCs after transplantation. Furthermore, nerve regeneration and functional recovery were promoted by the synergism of SCs-loaded MGs and MF. Based on the current findings, the combined application of MGs and SCs with applied MF is a promising therapy for the engineering of peripheral nerve regeneration. Keywords: peripheral nerve repair, magnetic nanoparticle, nanocomposite, magnetic field, Schwann cell, functional recovery
format	article
author	Liu ZY Zhu S Liu L Ge J Huang LL Sun Z Zeng W Huang JH Luo ZJ
author_facet	Liu ZY Zhu S Liu L Ge J Huang LL Sun Z Zeng W Huang JH Luo ZJ
author_sort	Liu ZY
title	A magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field
title_short	A magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field
title_full	A magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field
title_fullStr	A magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field
title_full_unstemmed	A magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field
title_sort	magnetically responsive nanocomposite scaffold combined with schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/7b204bbc33784834a07f34955e971865
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A magnetically responsive nanocomposite scaffold combined with Schwann cells promotes sciatic nerve regeneration upon exposure to magnetic field

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