<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO

The androgen receptor (AR) and its related signaling pathways play an important role in the development of prostate cancer (PCa). Long non-coding RNAs (lncRNAs) are involved in the regulation of tumorigenesis and development, but their specific mechanism of action remains unclear. This study examine...

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Autores principales:	Yali Lu, Xuechao Wan, Wenhua Huang, Lu Zhang, Jun Luo, Dujian Li, Yan Huang, Yao Li, Yaoting Xu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	prostate cancer androgen receptor <i>AC016745.3</i> NONO transcriptional regulation Science Q
Acceso en línea:	https://doaj.org/article/7b223a770eb44a31a300146ebe6735d5
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spelling	oai:doaj.org-article:7b223a770eb44a31a300146ebe6735d52021-11-25T18:11:12Z<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO10.3390/life111112082075-1729https://doaj.org/article/7b223a770eb44a31a300146ebe6735d52021-11-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1208https://doaj.org/toc/2075-1729The androgen receptor (AR) and its related signaling pathways play an important role in the development of prostate cancer (PCa). Long non-coding RNAs (lncRNAs) are involved in the regulation of tumorigenesis and development, but their specific mechanism of action remains unclear. This study examines the function and mechanisms of action of lncRNA <i>AC016745.3</i> in the development of PCa. It shows that dihydrotestosterone (DHT) results in the AR-dependent suppression of <i>AC016745.3</i> expression in the LNCaP androgen-sensitive human prostate adenocarcinoma cell line. In addition, overexpression of <i>AC016745.3</i> inhibits the proliferation and migration of PCa cells, and suppresses the expression of AR target genes. This research also demonstrates that the protein NONO interacts with AR and functions as an AR co-activator, promoting AR transcriptional activity. Furthermore, using RNA immunoprecipitation (RIP)-PCR experiments, the study demonstrates that both NONO and AR can bind <i>AC016745.3</i>. Moreover, cell phenotypic experiments reveal that NONO can promote cellular proliferation and migration, and that <i>AC016745.3</i> can partially antagonize the pro-oncogenic functions of NONO in PCa cells. In summary, the results indicate that <i>AC016745.3</i> can bind NONO, suppressing its ability to promote AR-dependent transcriptional activity. Furthermore, DHT-dependent suppression of <i>AC016745.3</i> expression can enhance NONO’s promotion effect on AR.Yali LuXuechao WanWenhua HuangLu ZhangJun LuoDujian LiYan HuangYao LiYaoting XuMDPI AGarticleprostate cancerandrogen receptor<i>AC016745.3</i>NONOtranscriptional regulationScienceQENLife, Vol 11, Iss 1208, p 1208 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	prostate cancer androgen receptor <i>AC016745.3</i> NONO transcriptional regulation Science Q
spellingShingle	prostate cancer androgen receptor <i>AC016745.3</i> NONO transcriptional regulation Science Q Yali Lu Xuechao Wan Wenhua Huang Lu Zhang Jun Luo Dujian Li Yan Huang Yao Li Yaoting Xu <i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO
description	The androgen receptor (AR) and its related signaling pathways play an important role in the development of prostate cancer (PCa). Long non-coding RNAs (lncRNAs) are involved in the regulation of tumorigenesis and development, but their specific mechanism of action remains unclear. This study examines the function and mechanisms of action of lncRNA <i>AC016745.3</i> in the development of PCa. It shows that dihydrotestosterone (DHT) results in the AR-dependent suppression of <i>AC016745.3</i> expression in the LNCaP androgen-sensitive human prostate adenocarcinoma cell line. In addition, overexpression of <i>AC016745.3</i> inhibits the proliferation and migration of PCa cells, and suppresses the expression of AR target genes. This research also demonstrates that the protein NONO interacts with AR and functions as an AR co-activator, promoting AR transcriptional activity. Furthermore, using RNA immunoprecipitation (RIP)-PCR experiments, the study demonstrates that both NONO and AR can bind <i>AC016745.3</i>. Moreover, cell phenotypic experiments reveal that NONO can promote cellular proliferation and migration, and that <i>AC016745.3</i> can partially antagonize the pro-oncogenic functions of NONO in PCa cells. In summary, the results indicate that <i>AC016745.3</i> can bind NONO, suppressing its ability to promote AR-dependent transcriptional activity. Furthermore, DHT-dependent suppression of <i>AC016745.3</i> expression can enhance NONO’s promotion effect on AR.
format	article
author	Yali Lu Xuechao Wan Wenhua Huang Lu Zhang Jun Luo Dujian Li Yan Huang Yao Li Yaoting Xu
author_facet	Yali Lu Xuechao Wan Wenhua Huang Lu Zhang Jun Luo Dujian Li Yan Huang Yao Li Yaoting Xu
author_sort	Yali Lu
title	<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO
title_short	<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO
title_full	<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO
title_fullStr	<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO
title_full_unstemmed	<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO
title_sort	<i>ac016745.3</i> regulates the transcription of ar target genes by antagonizing nono
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/7b223a770eb44a31a300146ebe6735d5
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<i>AC016745.3</i> Regulates the Transcription of AR Target Genes by Antagonizing NONO

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