Age of the donor affects the nature of in vitro cultured human dental pulp stem cells

Objectives: The dental pulp stem cells (DPSCs) of six donors (three young donors aged < 19 years and three adult donors aged > 25 and < 30 years) were characterized for their stem cell marker expression and differentiation potential to study the effect of donor age on DPSCs in vitro. Method...

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Autores principales:	Heba Alzer, Heba Kalbouneh, Firas Alsoleihat, Nisreen Abu Shahin, Soukaina Ryalat, Mohammad Alsalem, Hazem Alahmad, Lubna Tahtamouni
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Dental pulp stem cells Neural crest stem cells Culture conditions Mesenchymal stem cells Donor age effect Medicine R Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/7b250e1b6efe4db7ac93be2d16a19e18
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spelling	oai:doaj.org-article:7b250e1b6efe4db7ac93be2d16a19e182021-11-10T04:19:44ZAge of the donor affects the nature of in vitro cultured human dental pulp stem cells1013-905210.1016/j.sdentj.2020.09.003https://doaj.org/article/7b250e1b6efe4db7ac93be2d16a19e182021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1013905220307719https://doaj.org/toc/1013-9052Objectives: The dental pulp stem cells (DPSCs) of six donors (three young donors aged < 19 years and three adult donors aged > 25 and < 30 years) were characterized for their stem cell marker expression and differentiation potential to study the effect of donor age on DPSCs in vitro. Methods: DPSCs were cultured in αMEM supplemented with 20% fetal calf serum (conventional conditions) or on fibronectin-coated flasks with neurobasal medium supplemented with B27, bFGF and EGF (alternative conditions). DPSCs were characterized by immunofluorescence staining to detect the neural crest/mesenchymal stem cells markers P75 and CD146, respectively. The differentiation potential was tested by the induction of DPSCs into osteogenic, adipogenic and glial lineages and then by detecting the corresponding markers osteocalcin, lipidtox and S100ß, respectively. Results: The DPSCs of the young donors expressed CD146 only under the conventional conditions and expressed P75 regardless of the culture conditions. However, the DPSCs of adult donors expressed CD146 only under the alternative conditions and expressed P75 only under conventional conditions. Only the DPSCs of the young donors differentiated into the glial linage. The DPSCs of the adult donors differentiated more efficiently into the adipogenic linage. Osteogenic differentiation was comparable. Conclusion: Donor age affects the expression of stem cell markers and differentiation potential of DPSCs. Moreover, the effect of culture conditions on DPSCs is age dependent.Heba AlzerHeba KalbounehFiras AlsoleihatNisreen Abu ShahinSoukaina RyalatMohammad AlsalemHazem AlahmadLubna TahtamouniElsevierarticleDental pulp stem cellsNeural crest stem cellsCulture conditionsMesenchymal stem cellsDonor age effectMedicineRDentistryRK1-715ENSaudi Dental Journal, Vol 33, Iss 7, Pp 524-532 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Dental pulp stem cells Neural crest stem cells Culture conditions Mesenchymal stem cells Donor age effect Medicine R Dentistry RK1-715
spellingShingle	Dental pulp stem cells Neural crest stem cells Culture conditions Mesenchymal stem cells Donor age effect Medicine R Dentistry RK1-715 Heba Alzer Heba Kalbouneh Firas Alsoleihat Nisreen Abu Shahin Soukaina Ryalat Mohammad Alsalem Hazem Alahmad Lubna Tahtamouni Age of the donor affects the nature of in vitro cultured human dental pulp stem cells
description	Objectives: The dental pulp stem cells (DPSCs) of six donors (three young donors aged < 19 years and three adult donors aged > 25 and < 30 years) were characterized for their stem cell marker expression and differentiation potential to study the effect of donor age on DPSCs in vitro. Methods: DPSCs were cultured in αMEM supplemented with 20% fetal calf serum (conventional conditions) or on fibronectin-coated flasks with neurobasal medium supplemented with B27, bFGF and EGF (alternative conditions). DPSCs were characterized by immunofluorescence staining to detect the neural crest/mesenchymal stem cells markers P75 and CD146, respectively. The differentiation potential was tested by the induction of DPSCs into osteogenic, adipogenic and glial lineages and then by detecting the corresponding markers osteocalcin, lipidtox and S100ß, respectively. Results: The DPSCs of the young donors expressed CD146 only under the conventional conditions and expressed P75 regardless of the culture conditions. However, the DPSCs of adult donors expressed CD146 only under the alternative conditions and expressed P75 only under conventional conditions. Only the DPSCs of the young donors differentiated into the glial linage. The DPSCs of the adult donors differentiated more efficiently into the adipogenic linage. Osteogenic differentiation was comparable. Conclusion: Donor age affects the expression of stem cell markers and differentiation potential of DPSCs. Moreover, the effect of culture conditions on DPSCs is age dependent.
format	article
author	Heba Alzer Heba Kalbouneh Firas Alsoleihat Nisreen Abu Shahin Soukaina Ryalat Mohammad Alsalem Hazem Alahmad Lubna Tahtamouni
author_facet	Heba Alzer Heba Kalbouneh Firas Alsoleihat Nisreen Abu Shahin Soukaina Ryalat Mohammad Alsalem Hazem Alahmad Lubna Tahtamouni
author_sort	Heba Alzer
title	Age of the donor affects the nature of in vitro cultured human dental pulp stem cells
title_short	Age of the donor affects the nature of in vitro cultured human dental pulp stem cells
title_full	Age of the donor affects the nature of in vitro cultured human dental pulp stem cells
title_fullStr	Age of the donor affects the nature of in vitro cultured human dental pulp stem cells
title_full_unstemmed	Age of the donor affects the nature of in vitro cultured human dental pulp stem cells
title_sort	age of the donor affects the nature of in vitro cultured human dental pulp stem cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7b250e1b6efe4db7ac93be2d16a19e18
work_keys_str_mv	AT hebaalzer ageofthedonoraffectsthenatureofinvitroculturedhumandentalpulpstemcells AT hebakalbouneh ageofthedonoraffectsthenatureofinvitroculturedhumandentalpulpstemcells AT firasalsoleihat ageofthedonoraffectsthenatureofinvitroculturedhumandentalpulpstemcells AT nisreenabushahin ageofthedonoraffectsthenatureofinvitroculturedhumandentalpulpstemcells AT soukainaryalat ageofthedonoraffectsthenatureofinvitroculturedhumandentalpulpstemcells AT mohammadalsalem ageofthedonoraffectsthenatureofinvitroculturedhumandentalpulpstemcells AT hazemalahmad ageofthedonoraffectsthenatureofinvitroculturedhumandentalpulpstemcells AT lubnatahtamouni ageofthedonoraffectsthenatureofinvitroculturedhumandentalpulpstemcells
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Age of the donor affects the nature of in vitro cultured human dental pulp stem cells

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