Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells

Abstract The ability of cancer cells to undergo partial-epithelial mesenchymal transition (p-EMT), rather than complete EMT, poses a higher metastatic risk. Although Fusobacterium nucleatum mainly inhabits in oral cavity, attention has been focused on the F. nucleatum involvement in colorectal cance...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wenhua Shao, Natsumi Fujiwara, Yasuhiro Mouri, Satoru Kisoda, Kayo Yoshida, Kaya Yoshida, Hiromichi Yumoto, Kazumi Ozaki, Naozumi Ishimaru, Yasusei Kudo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b264f4195b34e1785df74634c32589b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7b264f4195b34e1785df74634c32589b
record_format	dspace
spelling	oai:doaj.org-article:7b264f4195b34e1785df74634c32589b2021-12-02T16:26:22ZConversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells10.1038/s41598-021-94384-12045-2322https://doaj.org/article/7b264f4195b34e1785df74634c32589b2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94384-1https://doaj.org/toc/2045-2322Abstract The ability of cancer cells to undergo partial-epithelial mesenchymal transition (p-EMT), rather than complete EMT, poses a higher metastatic risk. Although Fusobacterium nucleatum mainly inhabits in oral cavity, attention has been focused on the F. nucleatum involvement in colorectal cancer development. Here we examined the p-EMT regulation by F. nucleatum in oral squamous cell carcinoma (OSCC) cells. We cultured OSCC cells with epithelial, p-EMT or EMT phenotype with live or heat-inactivated F. nucleatum. Expression of the genes involved in epithelial differentiation, p-EMT and EMT were examined in OSCC cells after co-culture with F. nucleatum by qPCR. Cell growth and invasion of OSCC cells were also examined. Both live and heat-inactivated F. nucleatum upregulated the expression of p-EMT-related genes in OSCC cells with epithelial phenotype, but not with p-EMT or EMT phenotype. Moreover, F. nucleatum promoted invasion of OSCC cells with epithelial phenotype. Co-culture with other strains of bacteria other than Porphyromonas gingivalis did not alter p-EMT-related genes in OSCC cells with epithelial phenotype. F. nucleatum infection may convert epithelial to p-EMT phenotype via altering gene expression in OSCC. Oral hygiene managements against F. nucleatum infection may contribute to reduce the risk for an increase in metastatic ability of OSCC.Wenhua ShaoNatsumi FujiwaraYasuhiro MouriSatoru KisodaKayo YoshidaKaya YoshidaHiromichi YumotoKazumi OzakiNaozumi IshimaruYasusei KudoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wenhua Shao Natsumi Fujiwara Yasuhiro Mouri Satoru Kisoda Kayo Yoshida Kaya Yoshida Hiromichi Yumoto Kazumi Ozaki Naozumi Ishimaru Yasusei Kudo Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells
description	Abstract The ability of cancer cells to undergo partial-epithelial mesenchymal transition (p-EMT), rather than complete EMT, poses a higher metastatic risk. Although Fusobacterium nucleatum mainly inhabits in oral cavity, attention has been focused on the F. nucleatum involvement in colorectal cancer development. Here we examined the p-EMT regulation by F. nucleatum in oral squamous cell carcinoma (OSCC) cells. We cultured OSCC cells with epithelial, p-EMT or EMT phenotype with live or heat-inactivated F. nucleatum. Expression of the genes involved in epithelial differentiation, p-EMT and EMT were examined in OSCC cells after co-culture with F. nucleatum by qPCR. Cell growth and invasion of OSCC cells were also examined. Both live and heat-inactivated F. nucleatum upregulated the expression of p-EMT-related genes in OSCC cells with epithelial phenotype, but not with p-EMT or EMT phenotype. Moreover, F. nucleatum promoted invasion of OSCC cells with epithelial phenotype. Co-culture with other strains of bacteria other than Porphyromonas gingivalis did not alter p-EMT-related genes in OSCC cells with epithelial phenotype. F. nucleatum infection may convert epithelial to p-EMT phenotype via altering gene expression in OSCC. Oral hygiene managements against F. nucleatum infection may contribute to reduce the risk for an increase in metastatic ability of OSCC.
format	article
author	Wenhua Shao Natsumi Fujiwara Yasuhiro Mouri Satoru Kisoda Kayo Yoshida Kaya Yoshida Hiromichi Yumoto Kazumi Ozaki Naozumi Ishimaru Yasusei Kudo
author_facet	Wenhua Shao Natsumi Fujiwara Yasuhiro Mouri Satoru Kisoda Kayo Yoshida Kaya Yoshida Hiromichi Yumoto Kazumi Ozaki Naozumi Ishimaru Yasusei Kudo
author_sort	Wenhua Shao
title	Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells
title_short	Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells
title_full	Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells
title_fullStr	Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells
title_full_unstemmed	Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells
title_sort	conversion from epithelial to partial-emt phenotype by fusobacterium nucleatum infection promotes invasion of oral cancer cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7b264f4195b34e1785df74634c32589b
work_keys_str_mv	AT wenhuashao conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT natsumifujiwara conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT yasuhiromouri conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT satorukisoda conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT kayoyoshida conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT kayayoshida conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT hiromichiyumoto conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT kazumiozaki conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT naozumiishimaru conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells AT yasuseikudo conversionfromepithelialtopartialemtphenotypebyfusobacteriumnucleatuminfectionpromotesinvasionoforalcancercells
_version_	1718384060756131840

Conversion from epithelial to partial-EMT phenotype by Fusobacterium nucleatum infection promotes invasion of oral cancer cells

Ejemplares similares