Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

Elena Filová,1 Tomá&scaron; Suchý,2,3 Zbynek Sucharda,2 Monika &Scaron;upová,2 Margit Žaloudková,2 Karel Balík,2 Vera Lisá,1 Miroslav &Scaron;louf,4 Lucie Bacáková11Department of Biomateri...

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Autores principales:	Filová E, Suchý T, Sucharda Z, &Scaron;upová M, Žaloudková M, Balík K, Lisá V, &Scaron;louf M, Bačáková L
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7b2784f6f8d941c1b0199cb1230f1297
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spelling	oai:doaj.org-article:7b2784f6f8d941c1b0199cb1230f12972021-12-02T03:11:42ZSupport for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites1178-2013https://doaj.org/article/7b2784f6f8d941c1b0199cb1230f12972014-08-01T00:00:00Zhttp://www.dovepress.com/support-for-the-initial-attachment-growth-and-differentiation-of-mg-63-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Elena Filová,1 Tomá&scaron; Suchý,2,3 Zbynek Sucharda,2 Monika &Scaron;upová,2 Margit Žaloudková,2 Karel Balík,2 Vera Lisá,1 Miroslav &Scaron;louf,4 Lucie Bacáková11Department of Biomaterials and Tissue Engineering, Institute of Physiology, 2Department of Composite and Carbon Materials, Institute of Rock Structure and Mechanics, Academy of Sciences of the Czech Republic, 3Laboratory of Biomechanics, Department of Mechanics, Biomechanics and Mechatronics, Faculty of Mechanical Engineering, CTU in Prague, 4Department of Morphology and Rheology of Polymer Materials, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech RepublicAbstract: Hydroxyapatite (HA) is considered to be a bioactive material that favorably influences the adhesion, growth, and osteogenic differentiation of osteoblasts. To optimize the cell response on the hydroxyapatite composite, it is desirable to assess the optimum concentration and also the optimum particle size. The aim of our study was to prepare composite materials made of polydimethylsiloxane, polyamide, and nano-sized (N) or micro-sized (M) HA, with an HA content of 0%, 2%, 5%, 10%, 15%, 20%, 25% (v/v) (referred to as N0–N25 or M0–M25), and to evaluate them in vitro in cultures with human osteoblast-like MG-63 cells. For clinical applications, fast osseointegration of the implant into the bone is essential. We observed the greatest initial cell adhesion on composites M10 and N5. Nano-sized HA supported cell growth, especially during the first 3 days of culture. On composites with micro-size HA (2%–15%), MG-63 cells reached the highest densities on day 7. Samples M20 and M25, however, were toxic for MG-63 cells, although these composites supported the production of osteocalcin in these cells. On N2, a higher concentration of osteopontin was found in MG-63 cells. For biomedical applications, the concentration range of 5%–15% (v/v) nano-size or micro-size HA seems to be optimum.Keywords: nanoparticles, hydroxyapatite, osteoblasts, composite materialsFilová ESuchý TSucharda Z&Scaron;upová MŽaloudková MBalík KLisá V&Scaron;louf MBačáková LDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2014, Iss Issue 1, Pp 3687-3706 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Filová E Suchý T Sucharda Z &Scaron;upová M Žaloudková M Balík K Lisá V &Scaron;louf M Bačáková L Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites
description	Elena Filová,1 Tomá&scaron; Suchý,2,3 Zbynek Sucharda,2 Monika &Scaron;upová,2 Margit Žaloudková,2 Karel Balík,2 Vera Lisá,1 Miroslav &Scaron;louf,4 Lucie Bacáková11Department of Biomaterials and Tissue Engineering, Institute of Physiology, 2Department of Composite and Carbon Materials, Institute of Rock Structure and Mechanics, Academy of Sciences of the Czech Republic, 3Laboratory of Biomechanics, Department of Mechanics, Biomechanics and Mechatronics, Faculty of Mechanical Engineering, CTU in Prague, 4Department of Morphology and Rheology of Polymer Materials, Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech RepublicAbstract: Hydroxyapatite (HA) is considered to be a bioactive material that favorably influences the adhesion, growth, and osteogenic differentiation of osteoblasts. To optimize the cell response on the hydroxyapatite composite, it is desirable to assess the optimum concentration and also the optimum particle size. The aim of our study was to prepare composite materials made of polydimethylsiloxane, polyamide, and nano-sized (N) or micro-sized (M) HA, with an HA content of 0%, 2%, 5%, 10%, 15%, 20%, 25% (v/v) (referred to as N0–N25 or M0–M25), and to evaluate them in vitro in cultures with human osteoblast-like MG-63 cells. For clinical applications, fast osseointegration of the implant into the bone is essential. We observed the greatest initial cell adhesion on composites M10 and N5. Nano-sized HA supported cell growth, especially during the first 3 days of culture. On composites with micro-size HA (2%–15%), MG-63 cells reached the highest densities on day 7. Samples M20 and M25, however, were toxic for MG-63 cells, although these composites supported the production of osteocalcin in these cells. On N2, a higher concentration of osteopontin was found in MG-63 cells. For biomedical applications, the concentration range of 5%–15% (v/v) nano-size or micro-size HA seems to be optimum.Keywords: nanoparticles, hydroxyapatite, osteoblasts, composite materials
format	article
author	Filová E Suchý T Sucharda Z &Scaron;upová M Žaloudková M Balík K Lisá V &Scaron;louf M Bačáková L
author_facet	Filová E Suchý T Sucharda Z &Scaron;upová M Žaloudková M Balík K Lisá V &Scaron;louf M Bačáková L
author_sort	Filová E
title	Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites
title_short	Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites
title_full	Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites
title_fullStr	Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites
title_full_unstemmed	Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites
title_sort	support for the initial attachment, growth and differentiation of mg-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/7b2784f6f8d941c1b0199cb1230f1297
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Support for the initial attachment, growth and differentiation of MG-63 cells: a comparison between nano-size hydroxyapatite and micro-size hydroxyapatite in composites

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