PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering

Jun Chen1,2, Beth Zhou1&ndash;3, Qi Li1,2, Jun Ouyang4, Jiming Kong2,4,5, Wen Zhong3,6, Malcolm MQ Xing1,2,4,71Department of Mechanical Engineering, Faculty of Engineering, University of Manitoba, Winnipeg, MB, Canada; 2Manitoba Institute of Child Health, Winnipeg, MB, Canada; 3Department of...

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Autores principales:	Chen J, Zhou B, Li Q, Ouyang J, Kong J, Zhong W, Xing MM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/7b2edcd1c38c466fbf64c79823a360ee
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spelling	oai:doaj.org-article:7b2edcd1c38c466fbf64c79823a360ee2021-12-02T03:01:53ZPLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering1176-91141178-2013https://doaj.org/article/7b2edcd1c38c466fbf64c79823a360ee2011-10-01T00:00:00Zhttp://www.dovepress.com/plla-peg-tch-labeled-bioactive-molecule-nanofibers-for-tissue-engineer-a8527https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jun Chen1,2, Beth Zhou1&ndash;3, Qi Li1,2, Jun Ouyang4, Jiming Kong2,4,5, Wen Zhong3,6, Malcolm MQ Xing1,2,4,71Department of Mechanical Engineering, Faculty of Engineering, University of Manitoba, Winnipeg, MB, Canada; 2Manitoba Institute of Child Health, Winnipeg, MB, Canada; 3Department of Textile Sciences, Faculty of Human Ecology, University of Manitoba, Winnipeg, MB, Canada; 4School of Basic Medical Science, Southern Medical University, Guangzhoug, China; 5Department of Human Anatomy and Cell Sciences, 6Department of Medical Microbiology, Faculty of Medicine, 7Department of Biochemistry and Medical Genetics, Faculty of Medicine, University of Manitoba, Winnipeg, MB, CanadaAbstract: By mimicking the native extracellular matrix, electrospun nanofibrous scaffolds (ENSs) can provide both chemical and physical cues to modulate cell adherence and differentiation and to promote tissue regeneration while retaining bioresorbable and biocompatible properties. In this study, ENSs were developed to deliver multiple biomolecules by loading them into the core-sheath structure and/or by conjugating them to the nanofiber surfaces. In this work, poly(L-lactide)-poly(ethylene glycol)-NH2 and poly(L-lactide) were emulsion electrospun into nanofibers with a core-sheath structure. A model drug, tetracycline hydrochloride, was loaded within the nanofibers. Amino and carboxyl reactive groups were then activated on the fiber surfaces using saturated water vapor exposure and base hydrolysis, respectively. These reactive groups allowed the surface of the ENS to be functionalized with two other bioactive molecules, fluorescein isothiocyanate- and rhodamine-labeled bovine serum albumins, which were used as model proteins. The ENSs were shown to retain their antimicrobial capacity after two functionalization reactions, indicating that multifunctional nanofibers can potentially be developed into functional wound dressings or periodontal membranes or used in more complicated tissue systems where multiple growth factors and anti-infection precautions are critical for the successful implantation and regeneration of tissues.Keywords: multifunctional nanofibrous scaffold, drug delivery, antibioticsChen JZhou BLi QOuyang JKong JZhong WXing MMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 2533-2542 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Chen J Zhou B Li Q Ouyang J Kong J Zhong W Xing MM PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering
description	Jun Chen1,2, Beth Zhou1&ndash;3, Qi Li1,2, Jun Ouyang4, Jiming Kong2,4,5, Wen Zhong3,6, Malcolm MQ Xing1,2,4,71Department of Mechanical Engineering, Faculty of Engineering, University of Manitoba, Winnipeg, MB, Canada; 2Manitoba Institute of Child Health, Winnipeg, MB, Canada; 3Department of Textile Sciences, Faculty of Human Ecology, University of Manitoba, Winnipeg, MB, Canada; 4School of Basic Medical Science, Southern Medical University, Guangzhoug, China; 5Department of Human Anatomy and Cell Sciences, 6Department of Medical Microbiology, Faculty of Medicine, 7Department of Biochemistry and Medical Genetics, Faculty of Medicine, University of Manitoba, Winnipeg, MB, CanadaAbstract: By mimicking the native extracellular matrix, electrospun nanofibrous scaffolds (ENSs) can provide both chemical and physical cues to modulate cell adherence and differentiation and to promote tissue regeneration while retaining bioresorbable and biocompatible properties. In this study, ENSs were developed to deliver multiple biomolecules by loading them into the core-sheath structure and/or by conjugating them to the nanofiber surfaces. In this work, poly(L-lactide)-poly(ethylene glycol)-NH2 and poly(L-lactide) were emulsion electrospun into nanofibers with a core-sheath structure. A model drug, tetracycline hydrochloride, was loaded within the nanofibers. Amino and carboxyl reactive groups were then activated on the fiber surfaces using saturated water vapor exposure and base hydrolysis, respectively. These reactive groups allowed the surface of the ENS to be functionalized with two other bioactive molecules, fluorescein isothiocyanate- and rhodamine-labeled bovine serum albumins, which were used as model proteins. The ENSs were shown to retain their antimicrobial capacity after two functionalization reactions, indicating that multifunctional nanofibers can potentially be developed into functional wound dressings or periodontal membranes or used in more complicated tissue systems where multiple growth factors and anti-infection precautions are critical for the successful implantation and regeneration of tissues.Keywords: multifunctional nanofibrous scaffold, drug delivery, antibiotics
format	article
author	Chen J Zhou B Li Q Ouyang J Kong J Zhong W Xing MM
author_facet	Chen J Zhou B Li Q Ouyang J Kong J Zhong W Xing MM
author_sort	Chen J
title	PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering
title_short	PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering
title_full	PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering
title_fullStr	PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering
title_full_unstemmed	PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering
title_sort	plla-peg-tch-labeled bioactive molecule nanofibers for tissue engineering
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/7b2edcd1c38c466fbf64c79823a360ee
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PLLA-PEG-TCH-labeled bioactive molecule nanofibers for tissue engineering

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