Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy

ABSTRACT Chronic tonsillitis (CT) and tonsillar hypertrophy (TH) are common tonsillar diseases that are related to infection and inflammation. Little is known about tonsillar microbiota and its role in CT and TH. This study aims to identify palatine tonsillar microbiota both on the surface and in th...

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Autores principales:	Shengru Wu, Lalle Hammarstedt-Nordenvall, Mattias Jangard, Liqin Cheng, Sebastian Alexandru Radu, Pia Angelidou, Yinghua Zha, Marica Hamsten, Lars Engstrand, Juan Du, Anders Ternhag
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2021
Materias:	chronic tonsillitis tonsillar hypertrophy tonsillar tissues microbiota 16S rRNA gene sequencing Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/7b3d66c81cb24e10a9f939c79eea8f9b
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spelling	oai:doaj.org-article:7b3d66c81cb24e10a9f939c79eea8f9b2021-12-02T17:07:47ZTonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy10.1128/mSystems.01302-202379-5077https://doaj.org/article/7b3d66c81cb24e10a9f939c79eea8f9b2021-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.01302-20https://doaj.org/toc/2379-5077ABSTRACT Chronic tonsillitis (CT) and tonsillar hypertrophy (TH) are common tonsillar diseases that are related to infection and inflammation. Little is known about tonsillar microbiota and its role in CT and TH. This study aims to identify palatine tonsillar microbiota both on the surface and in the core tissues of CT and TH patients. In total, 22 palatine tonsils were removed and collected from CT and TH patients who underwent surgery. The surface and core microbiota in the tonsils of CT and TH patients were compared using 16S rRNA gene sequencing of V3-V4 regions. Differential tonsillar microbiotas were found in the CT versus TH patients and surface versus core tissues. Further, a higher relative abundance of bacterial genera, including Haemophilus, Streptococcus, Neisseria, Capnocytophaga, Kingella, Moraxella, and Lachnospiraceae [G-2] in patients with TH and Dialister, Parvimonas, Bacteroidales [G-2], Aggregatibacter, and Atopobium in patients with CT, was observed. Of these, the differential genera of Dialister, Parvimonas, and Neisseria served as key factors in the tonsillar microbiota network. Notably, four representable tonsillar microbial types were identified, with one, consisting of a higher abundance of Haemophilus and Neisseria, exclusively detected in the TH patients. This study analyzed the different tonsillar microbiota from the surface and core tissues of CT and TH patients. Several bacteria and various microbial types related to CT and TH were identified, along with potential bacterial networks and related immune pathways. IMPORTANCE The human microbiota has been shown to be functionally connected to infectious and inflammation-related diseases. So far, only limited studies had been performed on tonsillar microbiota, although tonsils play an essential role in the human immune defense system and encountered numerous microorganisms. Our work presented different tonsillar microbiota from surface and core tissues of chronic tonsillitis (CT) and tonsillar hypertrophy (TH) patients. Notably, one tonsillar microbiota type, which contains a higher abundance of Haemophilus and Neisseria, was only detected in the TH patients. Furthermore, certain bacteria, such as Haemophilus, Neisseria, Dialister, and Parvimonas, may serve as microbial biomarkers to discriminate CT patients from TH patients. These data provide important microbiota data in the tonsillar research area and are highly useful for researchers both in the oral microbiome field and clinical field.Shengru WuLalle Hammarstedt-NordenvallMattias JangardLiqin ChengSebastian Alexandru RaduPia AngelidouYinghua ZhaMarica HamstenLars EngstrandJuan DuAnders TernhagAmerican Society for Microbiologyarticlechronic tonsillitistonsillar hypertrophytonsillar tissuesmicrobiota16S rRNA gene sequencingMicrobiologyQR1-502ENmSystems, Vol 6, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chronic tonsillitis tonsillar hypertrophy tonsillar tissues microbiota 16S rRNA gene sequencing Microbiology QR1-502
spellingShingle	chronic tonsillitis tonsillar hypertrophy tonsillar tissues microbiota 16S rRNA gene sequencing Microbiology QR1-502 Shengru Wu Lalle Hammarstedt-Nordenvall Mattias Jangard Liqin Cheng Sebastian Alexandru Radu Pia Angelidou Yinghua Zha Marica Hamsten Lars Engstrand Juan Du Anders Ternhag Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy
description	ABSTRACT Chronic tonsillitis (CT) and tonsillar hypertrophy (TH) are common tonsillar diseases that are related to infection and inflammation. Little is known about tonsillar microbiota and its role in CT and TH. This study aims to identify palatine tonsillar microbiota both on the surface and in the core tissues of CT and TH patients. In total, 22 palatine tonsils were removed and collected from CT and TH patients who underwent surgery. The surface and core microbiota in the tonsils of CT and TH patients were compared using 16S rRNA gene sequencing of V3-V4 regions. Differential tonsillar microbiotas were found in the CT versus TH patients and surface versus core tissues. Further, a higher relative abundance of bacterial genera, including Haemophilus, Streptococcus, Neisseria, Capnocytophaga, Kingella, Moraxella, and Lachnospiraceae [G-2] in patients with TH and Dialister, Parvimonas, Bacteroidales [G-2], Aggregatibacter, and Atopobium in patients with CT, was observed. Of these, the differential genera of Dialister, Parvimonas, and Neisseria served as key factors in the tonsillar microbiota network. Notably, four representable tonsillar microbial types were identified, with one, consisting of a higher abundance of Haemophilus and Neisseria, exclusively detected in the TH patients. This study analyzed the different tonsillar microbiota from the surface and core tissues of CT and TH patients. Several bacteria and various microbial types related to CT and TH were identified, along with potential bacterial networks and related immune pathways. IMPORTANCE The human microbiota has been shown to be functionally connected to infectious and inflammation-related diseases. So far, only limited studies had been performed on tonsillar microbiota, although tonsils play an essential role in the human immune defense system and encountered numerous microorganisms. Our work presented different tonsillar microbiota from surface and core tissues of chronic tonsillitis (CT) and tonsillar hypertrophy (TH) patients. Notably, one tonsillar microbiota type, which contains a higher abundance of Haemophilus and Neisseria, was only detected in the TH patients. Furthermore, certain bacteria, such as Haemophilus, Neisseria, Dialister, and Parvimonas, may serve as microbial biomarkers to discriminate CT patients from TH patients. These data provide important microbiota data in the tonsillar research area and are highly useful for researchers both in the oral microbiome field and clinical field.
format	article
author	Shengru Wu Lalle Hammarstedt-Nordenvall Mattias Jangard Liqin Cheng Sebastian Alexandru Radu Pia Angelidou Yinghua Zha Marica Hamsten Lars Engstrand Juan Du Anders Ternhag
author_facet	Shengru Wu Lalle Hammarstedt-Nordenvall Mattias Jangard Liqin Cheng Sebastian Alexandru Radu Pia Angelidou Yinghua Zha Marica Hamsten Lars Engstrand Juan Du Anders Ternhag
author_sort	Shengru Wu
title	Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy
title_short	Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy
title_full	Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy
title_fullStr	Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy
title_full_unstemmed	Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy
title_sort	tonsillar microbiota: a cross-sectional study of patients with chronic tonsillitis or tonsillar hypertrophy
publisher	American Society for Microbiology
publishDate	2021
url	https://doaj.org/article/7b3d66c81cb24e10a9f939c79eea8f9b
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Tonsillar Microbiota: a Cross-Sectional Study of Patients with Chronic Tonsillitis or Tonsillar Hypertrophy

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