Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time

Abstract Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation. However the utility of BRET is limited by the requir...

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Autores principales: Carl W. White, Hannah K. Vanyai, Heng B. See, Elizabeth K. M. Johnstone, Kevin D. G. Pfleger
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/7b3fcf6037e049f69f6a0bb8d24be1b0
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