Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal

Abstract Fluorescence imaging of tumours facilitates rapid intraoperative diagnosis. Thus far, a promising activatable fluorescence probe for hepatocellular carcinoma (HCC) has not been developed. Herein, the utility of the fluorescence imaging of HCC using a β-galactosidase (β-Gal)-activatable fluo...

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Autores principales:	Soichiro Ogawa, Hidemasa Kubo, Yasutoshi Murayama, Takeshi Kubota, Masayuki Yubakami, Tatsuya Matsumoto, Yusuke Yamamoto, Ryo Morimura, Hisashi Ikoma, Kazuma Okamoto, Mako Kamiya, Yasuteru Urano, Eigo Otsuji
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b41a7f1dcc847a382c4b42d574b5f68
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spelling	oai:doaj.org-article:7b41a7f1dcc847a382c4b42d574b5f682021-12-02T17:19:16ZRapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal10.1038/s41598-021-97073-12045-2322https://doaj.org/article/7b41a7f1dcc847a382c4b42d574b5f682021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97073-1https://doaj.org/toc/2045-2322Abstract Fluorescence imaging of tumours facilitates rapid intraoperative diagnosis. Thus far, a promising activatable fluorescence probe for hepatocellular carcinoma (HCC) has not been developed. Herein, the utility of the fluorescence imaging of HCC using a β-galactosidase (β-Gal)-activatable fluorescence probe SPiDER-βGal was examined. β-Gal activity was measured in cryopreserved tissues from 68 patients. Live cell imaging of HCC cell lines and imaging of tumour-bearing model mice were performed using SPiDER-βGal. Furthermore, fluorescence imaging was performed in 27 freshly resected human HCC specimens. In cryopreserved samples, β-Gal activity was significantly higher in tumour tissues than in non-tumour tissues. Fluorescence was observed in HCC cell lines. In mouse models, tumours displayed stronger fluorescence than normal liver tissue. In freshly resected specimens, fluorescence intensity in the tumour was significantly higher than that in non-tumour liver specimens as early as 2 min after spraying. Receiver operating characteristic curves were generated to determine the diagnostic value of SPiDER-βGal 10 min after its spraying; an area under the curve of 0.864, sensitivity of 85.2%, and specificity of 74.1% were observed for SPiDER-βGal. SPiDER-βGal is useful for the rapid fluorescence imaging of HCC. Fluorescence imaging guided by SPiDER-βGal would help surgeons detect tumours rapidly and achieve complete liver resection.Soichiro OgawaHidemasa KuboYasutoshi MurayamaTakeshi KubotaMasayuki YubakamiTatsuya MatsumotoYusuke YamamotoRyo MorimuraHisashi IkomaKazuma OkamotoMako KamiyaYasuteru UranoEigo OtsujiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Soichiro Ogawa Hidemasa Kubo Yasutoshi Murayama Takeshi Kubota Masayuki Yubakami Tatsuya Matsumoto Yusuke Yamamoto Ryo Morimura Hisashi Ikoma Kazuma Okamoto Mako Kamiya Yasuteru Urano Eigo Otsuji Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal
description	Abstract Fluorescence imaging of tumours facilitates rapid intraoperative diagnosis. Thus far, a promising activatable fluorescence probe for hepatocellular carcinoma (HCC) has not been developed. Herein, the utility of the fluorescence imaging of HCC using a β-galactosidase (β-Gal)-activatable fluorescence probe SPiDER-βGal was examined. β-Gal activity was measured in cryopreserved tissues from 68 patients. Live cell imaging of HCC cell lines and imaging of tumour-bearing model mice were performed using SPiDER-βGal. Furthermore, fluorescence imaging was performed in 27 freshly resected human HCC specimens. In cryopreserved samples, β-Gal activity was significantly higher in tumour tissues than in non-tumour tissues. Fluorescence was observed in HCC cell lines. In mouse models, tumours displayed stronger fluorescence than normal liver tissue. In freshly resected specimens, fluorescence intensity in the tumour was significantly higher than that in non-tumour liver specimens as early as 2 min after spraying. Receiver operating characteristic curves were generated to determine the diagnostic value of SPiDER-βGal 10 min after its spraying; an area under the curve of 0.864, sensitivity of 85.2%, and specificity of 74.1% were observed for SPiDER-βGal. SPiDER-βGal is useful for the rapid fluorescence imaging of HCC. Fluorescence imaging guided by SPiDER-βGal would help surgeons detect tumours rapidly and achieve complete liver resection.
format	article
author	Soichiro Ogawa Hidemasa Kubo Yasutoshi Murayama Takeshi Kubota Masayuki Yubakami Tatsuya Matsumoto Yusuke Yamamoto Ryo Morimura Hisashi Ikoma Kazuma Okamoto Mako Kamiya Yasuteru Urano Eigo Otsuji
author_facet	Soichiro Ogawa Hidemasa Kubo Yasutoshi Murayama Takeshi Kubota Masayuki Yubakami Tatsuya Matsumoto Yusuke Yamamoto Ryo Morimura Hisashi Ikoma Kazuma Okamoto Mako Kamiya Yasuteru Urano Eigo Otsuji
author_sort	Soichiro Ogawa
title	Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal
title_short	Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal
title_full	Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal
title_fullStr	Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal
title_full_unstemmed	Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal
title_sort	rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe spider-βgal
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7b41a7f1dcc847a382c4b42d574b5f68
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Rapid fluorescence imaging of human hepatocellular carcinoma using the β-galactosidase-activatable fluorescence probe SPiDER-βGal

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