Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.

Reactive oxygen species leads to some diseases associated with oxidative stress. Callicarpa kwangtungensis Chun (CK) is a common remedy in traditional Chinese medicine and possesses diverse biological activities involving antioxidant properties; its main compounds phenylethanoid glycosides (PG) and...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hao Cai, Zhiyong Xie, Guanghui Liu, Xiuman Sun, Guangtian Peng, Baoqin Lin, Qiongfeng Liao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b445076d5a349cda11bdcb4b9c8858a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:7b445076d5a349cda11bdcb4b9c8858a
record_format	dspace
spelling	oai:doaj.org-article:7b445076d5a349cda11bdcb4b9c8858a2021-11-18T08:26:22ZIsolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.1932-620310.1371/journal.pone.0093000https://doaj.org/article/7b445076d5a349cda11bdcb4b9c8858a2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24667350/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Reactive oxygen species leads to some diseases associated with oxidative stress. Callicarpa kwangtungensis Chun (CK) is a common remedy in traditional Chinese medicine and possesses diverse biological activities involving antioxidant properties; its main compounds phenylethanoid glycosides (PG) and flavonoids are always reported as antioxidants. In order to develop CK as a safe and activated antioxidant, our investigation was performed to validate antioxidant properties and assess which types of compounds (similar polarity or similar structure), even which compounds, played the role of antioxidants. The extracted compounds of CK were analyzed qualitatively and quantitatively by HPLC-DAD-ESI-Trap MS and UV for their contents and antioxidant activities. The correlations between antioxidant activities and known contents were respectively counted and a semi-quantitative experiment was designed to screen antioxidant compounds of CK with HPLC-UV. The n-butanol fraction (BF) showed the highest total phenolic and flavonoid contents (TPC, TFC), and three PG (forsythiaside B, poliumoside and acteoside) contents. BF showed the significantly best (P<0.05) activities in most assays. There were significant correlations (P<0.05) between DPPH•, ABTS(+)•, •O2(-) scavenging, Cu(2+)-chelating, anti-lipidperoxidation activities and TPC. BF also has significant antioxidant activities on CCl4-induced acute liver injury Mice and TBHP-reduced HepG2 cells. Nine PG (forsythiaside B, poliumoside, acteoside, alyssonoside, brandioside and their derivatives) and one flavone (rhamnazin) were screened out as antioxidants. BF in CK contained abundant polyphenolic, which reflected some definite antioxidant properties. The antioxidant compounds consisted at the least of nine PG and one flavone.Hao CaiZhiyong XieGuanghui LiuXiuman SunGuangtian PengBaoqin LinQiongfeng LiaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e93000 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hao Cai Zhiyong Xie Guanghui Liu Xiuman Sun Guangtian Peng Baoqin Lin Qiongfeng Liao Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.
description	Reactive oxygen species leads to some diseases associated with oxidative stress. Callicarpa kwangtungensis Chun (CK) is a common remedy in traditional Chinese medicine and possesses diverse biological activities involving antioxidant properties; its main compounds phenylethanoid glycosides (PG) and flavonoids are always reported as antioxidants. In order to develop CK as a safe and activated antioxidant, our investigation was performed to validate antioxidant properties and assess which types of compounds (similar polarity or similar structure), even which compounds, played the role of antioxidants. The extracted compounds of CK were analyzed qualitatively and quantitatively by HPLC-DAD-ESI-Trap MS and UV for their contents and antioxidant activities. The correlations between antioxidant activities and known contents were respectively counted and a semi-quantitative experiment was designed to screen antioxidant compounds of CK with HPLC-UV. The n-butanol fraction (BF) showed the highest total phenolic and flavonoid contents (TPC, TFC), and three PG (forsythiaside B, poliumoside and acteoside) contents. BF showed the significantly best (P<0.05) activities in most assays. There were significant correlations (P<0.05) between DPPH•, ABTS(+)•, •O2(-) scavenging, Cu(2+)-chelating, anti-lipidperoxidation activities and TPC. BF also has significant antioxidant activities on CCl4-induced acute liver injury Mice and TBHP-reduced HepG2 cells. Nine PG (forsythiaside B, poliumoside, acteoside, alyssonoside, brandioside and their derivatives) and one flavone (rhamnazin) were screened out as antioxidants. BF in CK contained abundant polyphenolic, which reflected some definite antioxidant properties. The antioxidant compounds consisted at the least of nine PG and one flavone.
format	article
author	Hao Cai Zhiyong Xie Guanghui Liu Xiuman Sun Guangtian Peng Baoqin Lin Qiongfeng Liao
author_facet	Hao Cai Zhiyong Xie Guanghui Liu Xiuman Sun Guangtian Peng Baoqin Lin Qiongfeng Liao
author_sort	Hao Cai
title	Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.
title_short	Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.
title_full	Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.
title_fullStr	Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.
title_full_unstemmed	Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.
title_sort	isolation, identification and activities of natural antioxidants from callicarpa kwangtungensis chun.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/7b445076d5a349cda11bdcb4b9c8858a
work_keys_str_mv	AT haocai isolationidentificationandactivitiesofnaturalantioxidantsfromcallicarpakwangtungensischun AT zhiyongxie isolationidentificationandactivitiesofnaturalantioxidantsfromcallicarpakwangtungensischun AT guanghuiliu isolationidentificationandactivitiesofnaturalantioxidantsfromcallicarpakwangtungensischun AT xiumansun isolationidentificationandactivitiesofnaturalantioxidantsfromcallicarpakwangtungensischun AT guangtianpeng isolationidentificationandactivitiesofnaturalantioxidantsfromcallicarpakwangtungensischun AT baoqinlin isolationidentificationandactivitiesofnaturalantioxidantsfromcallicarpakwangtungensischun AT qiongfengliao isolationidentificationandactivitiesofnaturalantioxidantsfromcallicarpakwangtungensischun
_version_	1718421811741327360

Isolation, identification and activities of natural antioxidants from Callicarpa kwangtungensis Chun.

Ejemplares similares