Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress

Plant metal tolerance proteins (MTPs) play major roles in enhancing resistance to heavy metal tolerance and homeostasis. However, the role of MTPs genes in tomato, which is one of the most popular crops, is still largely limited. Hence, we investigated genome-wide study of tomato MTPs, including phy...

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Autores principales:	Ahmed H. El- Sappah, Ahmed S. Elrys, El-Sayed M. Desoky, Xia Zhao, Wang Bingwen, Hamza H. El-Sappah, Yumin Zhu, Wanhai Zhou, Xianming Zhao, Jia Li
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Tomato Metal tolerance protein Heavy metals Genome-wide identification Gene expression Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/7b463865928f4118b8f332d62ae57927
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spelling	oai:doaj.org-article:7b463865928f4118b8f332d62ae579272021-11-20T04:56:34ZComprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress1319-562X10.1016/j.sjbs.2021.07.073https://doaj.org/article/7b463865928f4118b8f332d62ae579272021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006574https://doaj.org/toc/1319-562XPlant metal tolerance proteins (MTPs) play major roles in enhancing resistance to heavy metal tolerance and homeostasis. However, the role of MTPs genes in tomato, which is one of the most popular crops, is still largely limited. Hence, we investigated genome-wide study of tomato MTPs, including phylogenetic, duplication, gene structure, gene ontology and previous transcriptomic data analysis. Moreover, the MTPs expression behaviour under various heavy metals stress has rarely been investigated. In the current study, eleven MTP candidate genes were genome-wide identified and classified into three major groups; Mn-cation diffusion facilitators (CDFs), Fe/Zn-CDFs, and Zn-CDFs based on the phylogeny. Structural analysis of SlMTPs showed high gene similarity within the same group with cation_efflux or ZT_dimerdomains. Evolutionary analysis revealed that segmental duplication contributed to the expansion of the SlMTP family. Gene ontology further showed the vital roles of MTPs in metal-related processes. Tissue-specific expression profiling exhibited similar expression patterns in the same group, whereas gene expression varied among groups. The MTPs expression was evaluated after tomato treatments by five divalent heavy metals (Cd2+, Co2+, Mn2+, Zn2+, and Fe2+). SlMTP genes displayed differential responses in either plant leaves or roots under heavy metals treatments. Nine and ten SlMTPs responded to at least one metal ion treatment in leaves and roots, respectively. In addition SlMTP1, SlMTP3, SlMTP4, SlMTP8, SlMTP10 and SlMTP11 exhibited the highest expression responses in most of heavy metals treatments. Overall, our findings presented a standpoint on the evolution of MTPs and their evolution in tomato and paved the way for additional functional characterization under heavy metal toxicity.Ahmed H. El- SappahAhmed S. ElrysEl-Sayed M. DesokyXia ZhaoWang BingwenHamza H. El-SappahYumin ZhuWanhai ZhouXianming ZhaoJia LiElsevierarticleTomatoMetal tolerance proteinHeavy metalsGenome-wide identificationGene expressionBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 6946-6956 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Tomato Metal tolerance protein Heavy metals Genome-wide identification Gene expression Biology (General) QH301-705.5
spellingShingle	Tomato Metal tolerance protein Heavy metals Genome-wide identification Gene expression Biology (General) QH301-705.5 Ahmed H. El- Sappah Ahmed S. Elrys El-Sayed M. Desoky Xia Zhao Wang Bingwen Hamza H. El-Sappah Yumin Zhu Wanhai Zhou Xianming Zhao Jia Li Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
description	Plant metal tolerance proteins (MTPs) play major roles in enhancing resistance to heavy metal tolerance and homeostasis. However, the role of MTPs genes in tomato, which is one of the most popular crops, is still largely limited. Hence, we investigated genome-wide study of tomato MTPs, including phylogenetic, duplication, gene structure, gene ontology and previous transcriptomic data analysis. Moreover, the MTPs expression behaviour under various heavy metals stress has rarely been investigated. In the current study, eleven MTP candidate genes were genome-wide identified and classified into three major groups; Mn-cation diffusion facilitators (CDFs), Fe/Zn-CDFs, and Zn-CDFs based on the phylogeny. Structural analysis of SlMTPs showed high gene similarity within the same group with cation_efflux or ZT_dimerdomains. Evolutionary analysis revealed that segmental duplication contributed to the expansion of the SlMTP family. Gene ontology further showed the vital roles of MTPs in metal-related processes. Tissue-specific expression profiling exhibited similar expression patterns in the same group, whereas gene expression varied among groups. The MTPs expression was evaluated after tomato treatments by five divalent heavy metals (Cd2+, Co2+, Mn2+, Zn2+, and Fe2+). SlMTP genes displayed differential responses in either plant leaves or roots under heavy metals treatments. Nine and ten SlMTPs responded to at least one metal ion treatment in leaves and roots, respectively. In addition SlMTP1, SlMTP3, SlMTP4, SlMTP8, SlMTP10 and SlMTP11 exhibited the highest expression responses in most of heavy metals treatments. Overall, our findings presented a standpoint on the evolution of MTPs and their evolution in tomato and paved the way for additional functional characterization under heavy metal toxicity.
format	article
author	Ahmed H. El- Sappah Ahmed S. Elrys El-Sayed M. Desoky Xia Zhao Wang Bingwen Hamza H. El-Sappah Yumin Zhu Wanhai Zhou Xianming Zhao Jia Li
author_facet	Ahmed H. El- Sappah Ahmed S. Elrys El-Sayed M. Desoky Xia Zhao Wang Bingwen Hamza H. El-Sappah Yumin Zhu Wanhai Zhou Xianming Zhao Jia Li
author_sort	Ahmed H. El- Sappah
title	Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_short	Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_full	Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_fullStr	Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_full_unstemmed	Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress
title_sort	comprehensive genome wide identification and expression analysis of mtp gene family in tomato (solanum lycopersicum) under multiple heavy metal stress
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/7b463865928f4118b8f332d62ae57927
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Comprehensive genome wide identification and expression analysis of MTP gene family in tomato (Solanum lycopersicum) under multiple heavy metal stress

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