Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging

Abstract Slicing may cause the risk of cross-contamination in cucumber. In this study, knife inoculated with Escherichia coli (E. coli) was used to cut cucumbers, bioluminescence imaging (BLI) was used to visualize the possible distribution and internalization of E. coli during cutting and storage....

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Autores principales:	Yeting Sun, Xiaoyan Zhao, Xiulan Xu, Yue Ma, Hongyang Guan, Hao Liang, Dan Wang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/7b4d7b2c2f554a3787cce053520910d8
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spelling	oai:doaj.org-article:7b4d7b2c2f554a3787cce053520910d82021-12-02T15:02:48ZMonitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging10.1038/s41598-021-90584-x2045-2322https://doaj.org/article/7b4d7b2c2f554a3787cce053520910d82021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90584-xhttps://doaj.org/toc/2045-2322Abstract Slicing may cause the risk of cross-contamination in cucumber. In this study, knife inoculated with Escherichia coli (E. coli) was used to cut cucumbers, bioluminescence imaging (BLI) was used to visualize the possible distribution and internalization of E. coli during cutting and storage. Results showed that the initial two slices resulted in greater bacterial transfer. The bacterial transfer exhibited a fluctuating decay trend, E. coli was most distributed at the initial cutting site. The contaminated area on the surface of cucumber slices decreased during the storage period, which can be attributed to the death and internalization of E. coli. The maximum internalization distance of E. coli was about 2–3 mm, and did not further spread after 30 min from inoculation. Hence, our results provide useful information for risk management in both home and industrial environment.Yeting SunXiaoyan ZhaoXiulan XuYue MaHongyang GuanHao LiangDan WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yeting Sun Xiaoyan Zhao Xiulan Xu Yue Ma Hongyang Guan Hao Liang Dan Wang Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging
description	Abstract Slicing may cause the risk of cross-contamination in cucumber. In this study, knife inoculated with Escherichia coli (E. coli) was used to cut cucumbers, bioluminescence imaging (BLI) was used to visualize the possible distribution and internalization of E. coli during cutting and storage. Results showed that the initial two slices resulted in greater bacterial transfer. The bacterial transfer exhibited a fluctuating decay trend, E. coli was most distributed at the initial cutting site. The contaminated area on the surface of cucumber slices decreased during the storage period, which can be attributed to the death and internalization of E. coli. The maximum internalization distance of E. coli was about 2–3 mm, and did not further spread after 30 min from inoculation. Hence, our results provide useful information for risk management in both home and industrial environment.
format	article
author	Yeting Sun Xiaoyan Zhao Xiulan Xu Yue Ma Hongyang Guan Hao Liang Dan Wang
author_facet	Yeting Sun Xiaoyan Zhao Xiulan Xu Yue Ma Hongyang Guan Hao Liang Dan Wang
author_sort	Yeting Sun
title	Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging
title_short	Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging
title_full	Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging
title_fullStr	Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging
title_full_unstemmed	Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging
title_sort	monitoring of transfer and internalization of escherichia coli from inoculated knives to fresh cut cucumbers (cucumis sativus l.) using bioluminescence imaging
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/7b4d7b2c2f554a3787cce053520910d8
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Monitoring of transfer and internalization of Escherichia coli from inoculated knives to fresh cut cucumbers (Cucumis sativus L.) using bioluminescence imaging

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